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Mycosynthesis of novel lactone in foliar endophytic fungus isolated from Bixa orellana L.

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Abstract

There is a colossal demand for natural pigments and its applications in recent times. In the study, a novel lactone pigment was isolated from a predominant endophytic fungus residing in Bixa orellana L. (Bixaceae) leaves. The endophyte was identified as Fusarium verticillioides through morphological and molecular investigations. The optimum growth parameters of the endophyte for pigment production were at 33 ºC with pH 6.5 in dark. Through comprehensive spectroscopic studies, the structure of the isolated lactone was resolved and identified as (E)-3, 3-dimethyl-4-(pent-1-en-1-yl)-4-propyldihydrofuran-2(3H)-one. The acute oral toxicity study of the pigment investigated upon female Wistar rats indicated the median lethal dose (LD50) value above 1000 mg/kg body weight affirming safety. Thus, the red pigment from the isolated endophyte may be employed as a sustainable source for natural colorant in industries owing to its non-toxicity.

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Acknowledgement

The VIT management and School of Advanced Sciences (SAS, VIT, and Vellore) are greatly acknowledged for the infrastructure provided to carry out the research work.

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Authors and Affiliations

  1. School of Bio Sciences and Technology, VIT, Vellore, Tamil Nadu, 632014, India

    Sujithra Vijayakumar & Siva Ramamoorthy

  2. Department of Crop Physiology, University of Agricultural Science, Bangalore, Karnataka, 560065, India

    Rajani Raj & Uma Shaanker

  3. School of Advanced Sciences, VIT, Vellore, Tamil Nadu, 632014, India

    Akella Sivaramakrishna

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  1. Sujithra Vijayakumar
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  2. Rajani Raj
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  3. Uma Shaanker
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  4. Akella Sivaramakrishna
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  5. Siva Ramamoorthy
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Contributions

SR conceived and designed the experiment. SV performed the experiments and wrote the manuscript. RR helped in the fungus characterization part. US reviewed the manuscript. ASR helped in the chemical characterization part. All authors have read and approved the manuscript.

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Correspondence to Siva Ramamoorthy.

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Ethics approval statement

The approval was obtained from the Institutional Animal Ethical Committee of Vellore Institute of Technology, Vellore, Tamil nadu, India with Registration no. VIT/IAEC/13/Feb13/15, during its meeting held on 13 February 2017. The procedures used in the study adhere to the Organisation for Economic Co-operation and Development (OECD) guidelines no.423.

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Vijayakumar, S., Raj, R., Shaanker, U. et al. Mycosynthesis of novel lactone in foliar endophytic fungus isolated from Bixa orellana L.. 3 Biotech 11, 33 (2021). https://doi.org/10.1007/s13205-020-02566-x

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