Abstract
Although Escherichia coli has four hydrogenases, their definite roles in fermentation are still not clear. In this study, all the operon deletion mutants of E.coli hydrogenases (∆hya, ∆hyb, ∆hyc, or ∆hyf) were constructed to evaluate the hydrogen metabolism in comparison to their respective single-gene deletion mutants of large subunits (∆hyaB, ∆hybC, ∆hycE, and ∆hyfG). Besides the hyc operon mutant that expectedly showed no hydrogen synthesis, the hyb operon mutant showed low hydrogen production and demonstrated significantly reduced growth under anaerobic conditions. The present work also provided first-hand data where deleterious effects of operon deletion were compared with single-gene deletion mutations and the results showed that the former type of deletion was found to cause more prominent phenotypic effects than the latter one. Interestingly, hyb operon mutant was remarkably distinct from other operon mutants, specifically in its inability to utilize glucose under both aerobic and anaerobic conditions. Further studies on this mutant revealed a significant reduction of the total intracellular ATP and NADH concentrations, which could explain its impaired glucose metabolism. In this way, Hyd-2 was verified as crucial not only in glucose metabolism but also in energy balance and redox homeostasis of the cells. Furthermore, a decreased expression of glucose metabolism-associated genes, particularly ppc and pykA, indicated their regulation by hyb operon, and thereby, glucose consumption. Moreover, the transcriptional changes in this mutant indicated the wide genomic connectivity of hyb operon to other metabolisms.
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The authors thank the Inamori Foundation for supporting this study. The authors also thank Dr. Nurul Asyifah Mustapha and Ayaka Imabayashi for their skilled technical assistance and glucose work in the study, respectively.
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TM designed the study. Experiments and transcriptomic data analysis were done by CS. The manuscript was written by CS. Both the authors read and approved the manuscript.
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Shekhar, C., Maeda, T. Impaired glucose metabolism by deleting the operon of hydrogenase 2 in Escherichia coli. Arch Microbiol 204, 627 (2022). https://doi.org/10.1007/s00203-022-03245-6
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DOI: https://doi.org/10.1007/s00203-022-03245-6