Abstract
A Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LD5P10T was isolated from a root of Kalidium cuspidatum, in Tumd Right Banner, Inner Mongolia, China. The strain grew at 4–40 ℃ (optimum 30 ℃), and pH 5.0–10.0 (optimum pH 8.0), and in the presence of 0–16.0% (w/v) NaCl (optimum 2.0%). The strain was positive for catalase, and urease, and negative for nitrate reduction, and oxidase. The phylogenetic trees based on the 16S rRNA gene sequences and the whole genome sequence both revealed that strain LD5P10T clustered tightly with Corynebacterium glyciniphilum AJ 3170T and shared 98.1, 98.1, and < 98.1% of the 16S rRNA gene sequence similarities with strains C. glyciniphilum AJ 3170T, C. variabile DSM 20132T, and all the other current type strains. Strain LD5P10T contained MK-9 as the major respiratory quinone. Its major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphoglycolipid, two unidentified lipids, and two unidentified phospholipids. Its major fatty acids were C16:0 and C18:1 ω9c. The genomic DNA G + C content was 69.0%. The average nucleotide identity based on BLAST (ANIb), amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values of strain LD5P10T to C. glyciniphilum AJ 3170T and C. variabile DSM 20132T were 82.9 and 76.4%, 85.3 and 69.4%, and 25.8 and 20.9%, respectively. The phylogenetic, physiological, and phenotypic results allowed the discrimination of strain LD5P10T from its phylogenetic relatives. Corynebacterium kalidii sp. nov. is, therefore, proposed with strain LD5P10T (= CGMCC 1.19144T = JCM 35048T) as the type strain.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- AAI:
-
Average amino acid identity
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- TLC:
-
Thin-layer chromatography
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Acknowledgements
We would like to thank Professor Aron from the Hebrew University of Jerusalem for received assistance with the nomenclature.
Funding
This work was supported in part by Natural Science Foundation of Inner Mongolia Autonomous Region of China (2021MS03031), Inner Mongolia Science & Technology Plan (Grant No. 2020GG0034), and High-Level Talent Start-Up Research Project of Inner Mongolia University (No. 21800-5185133).
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JQS designed the research; LX isolated the strain; JYF performed the research; JYF, SKT, and JQS analyzed the data and wrote the paper. All authors reviewed the manuscript.
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Feng, JY., Xu, L., Tang, SK. et al. Corynebacterium kalidii sp. nov, an endophyte from a shoot of the halophyte Kalidium cuspidatum. Arch Microbiol 204, 471 (2022). https://doi.org/10.1007/s00203-022-03101-7
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DOI: https://doi.org/10.1007/s00203-022-03101-7