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Marinilactibacillus kalidii sp. nov., an Indole Acetic Acid-Producing Endophyte Isolated from a Shoot of Halophyte Kalidium cuspidatum

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Abstract

A Gram-stain-positive, facultatively anaerobic, non-sporulating, motile with single polar flagellum, rod-shaped, indole-3-acetic acid (IAA)-producing bacterium, named M4U5P12T, was isolated from a shoot of Kalidium cuspidatum, Inner Mongolia, China. Strain M4U5P12T grew at pH 6.0–11.0 (optimum 7.5), 4–40 °C (optimum 25 °C), and in the presence of 0–15% (w/v) NaCl (optimum 4%). Positive for catalase, urease, methyl red (M.R.) reaction, and hydrolysis of starch; and negative for oxidase, Voges–Proskauer (V–P) test, and hydrolysis of cellulose. The phylogenetic trees based on the 16S rRNA gene sequences and the whole genome sequences both revealed that it clustered with Marinilactibacillus piezotolerans JCM 12337T (99.3%) and Marinilactibacillus psychrotolerans M13-2T (99.1%). The dDDH and ANIb values of strain M4U5P12T to M. piezotolerans DSM 16108T and M. psychrotolerans M13-2T were 19.3 and 18.9%, and 74.3 and 74.0%, respectively. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, and two unidentified lipids. The major fatty acids were C16:0, C18:1 ω9c, C16:1 ω9c, and C15:1 ω5c. The genomic DNA G + C content was 37.3%. On the basis of physiological, phenotypic, and phylogenetic characteristics, strain M4U5P12T should be classified as a novel species. Therefore, Marinilactibacillus kalidii sp. nov. is proposed, and the type strain is M4U5P12T (= CGMCC 1.17696T = KCTC 43247T).

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Acknowledgements

We thank Jing-Nan Liang from Institute of Microbiology of CAS for morphologic observation using electron microscopic, and professor Aharon Oren from the Hebrew University of Jerusalem for naming the species. This work was supported in part by National Natural Science Foundation of China (Grant No. 31960020), Inner Mongolia Science & Technology Plan (Grant No. 2020GG0034), Natural Science Foundation of Inner Mongolia Autonomous Region of China (2021MS03031), and High-Level Talent Start-Up Research Project of Inner Mongolia University (No. 21800-5185133).

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  1. Xiao-Xian Huang and Lian Xu have contributed equally to this work.

Authors and Affiliations

  1. Laboratory for Microbial Resources, Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, People’s Republic of China

    Xiao-Xian Huang, Jia Shang & Ji-Quan Sun

  2. Jiangsu Key Laboratory for Organic Solid Waste Utilization, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Lian Xu

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Huang, XX., Xu, L., Shang, J. et al. Marinilactibacillus kalidii sp. nov., an Indole Acetic Acid-Producing Endophyte Isolated from a Shoot of Halophyte Kalidium cuspidatum. Curr Microbiol 79, 198 (2022). https://doi.org/10.1007/s00284-022-02894-6

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