Abstract
During overwintering of black-necked cranes (Grus nigricollis), the composition and function of the gut microbiota changes are of considerable interest for understanding its environmental adaption mechanism. In this study, we characterized the structure of the gut microbiota from the black-necked crane in the Dashanbao wintering area, and compared the early-winter (November) microbiota to the late-winter (March of the next year) microbiota. The results showed that the gut microbiota diversity of black-necked crane in the early-overwintering stage was higher than that in the late-overwintering stage, but it did not reach a significant level. Gut microbiota taxonomic composition analysis showed that relative abundance of Bacteroidota increased significantly, and showed decreased Firmicutes to Bacteroidota ratio at the phylum level, meanwhile, the abundance of Lactobacillus decreased significantly at the genus level. Explain gut microbiota between the early- and late-wintering showed some differences in microbiota richness but maintained a relatively conservative microbiota structure. PICRUSt2 method was used to predict and analyze the KEGG functional abundance of 16S rDNA sequences of bacteria, it was found that the changes in gut microbiota composition increased the abundance of bacteria associated with amino acid biosynthesis and acid metabolism in the late stage of overwintering. This work provides basic data for black-necked crane gut microbiota study, which might further contribute to their protection.
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Abbreviations
- ASV:
-
Amplicon sequence variants
- Chao1:
-
The Chao1 estimator
- LEfSe:
-
Linear discriminant analysis (LDA) effect size
- PICRUSt 2:
-
Phylogenetic investigation of communities by reconstruction of unobserved states 2
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- ABC transporters:
-
ATP-binding cassette transporters
- NSTI:
-
Nearest sequenced taxon index
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Acknowledgements
This study was supported in part by grants from the Basic research project of the Education Department of Yunnan Province, China (No. 2019j1142). The experiment was supported by Shanghai Majorbio Bio-pharm Technology Co., Ltd. Thanks for the support of Dashanbao National Nature Reserve of Yunnan Province for sample collection and Chen Guanghui, the staff member, for their assistance in the sample collection process.
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HX, JZ designed the experiment and wrote the first draft. YW, MZ, YY, HT, ZS and LW collected the fecal samples and performed preliminary preparation. All authors has helped in revision and approved the final manuscript.
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Zhao, J., Wang, Y., Zhang, M. et al. Structural changes in the gut microbiota community of the black-necked crane (Grus nigricollis) in the wintering period. Arch Microbiol 203, 6203–6214 (2021). https://doi.org/10.1007/s00203-021-02587-x
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DOI: https://doi.org/10.1007/s00203-021-02587-x