Abstract
The two novel bacterial strains designated 1Y17T and 4Y10T from aquaculture water were characterized using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains 1Y17T and 4Y10T belonged to the genus Inhella and were close to Inhella crocodyli CCP-18T, Inhella inkyongensis IMCC1713T and Inhella fonticola TNR-25T. Strains 1Y17T and 4Y10T shared 98.6% identity with each other and less than 99.0% identity with their relatives above. The phylogenomic analysis indicated that the two strains formed two independent branches distinct from their relatives. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the two strains were 21.3 and 80.9% below the two thresholds of 70% dDDH and 95–96% ANI for species definition; those between the two novel strains and their relatives were far below thresholds for species definition, implying that they represent two novel genospecies. The predominant fatty acids of the two strains were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C16:0; the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol; the major quinone and polyamine were Q-8 and putrescine. Their genomic DNA G + C contents were 69.3 and 65.0%. The two novel strains can produce poly-β-hydroxybutyrate, matching with the presence of the three synthetic related genes of the phaC-phaA-phaB in their genomes. Based on the genotypic and phenotypic characteristics such as aesculin and gelatin hydrolysis, strains 1Y17T and 4Y10T are concluded to represent two novel species of the genus Inhella, for which the names Inhella proteolytica sp. nov. (type strain 1Y17T = GDMCC 1.1830T = KACC 21948T) and Inhella gelatinilytica sp. (type strain 4Y10T = GDMCC 1.1829T = KCTC 82338T) are proposed.
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Acknowledgements
We are very grateful for Dr. Anzhang Li for the collection of aquaculture water, Mr. Jun Zhang for the help in the analyses of fatty acids, quinone, and polyamine, and Mr. Fan Yang for the acquisition of related type strains, and Ms. Yulian Zhang for the preservation of the two novel strains.
Funding
This work was jointly supported by the Key Realm R&D Program of Guangdong Province (2018B020205003), the GDAS’ Project of Science and Technology Development (2020GDASYL-20200103017), and the Science and Technology Plan Project of Guangdong Province (2019B030316017).
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Conceptualization: YL and HZ; data curation: YL; formal analysis: YL; funding acquisition: YL and HZ; investigation: YL, TP, JD, MD, and HZ; methodology: YL, TP, and JD; project administration: YL and HZ; software: YL; supervision and validation: YL and HZ; visualization: YL; writing—original draft: YL; writing—review and editing: YL and HZ.
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Liu, Y., Pei, T., Du, J. et al. Inhella proteolytica sp. nov. and Inhella gelatinilytica sp. nov., two novel species of the genus Inhella isolated from aquaculture water. Arch Microbiol 203, 3191–3200 (2021). https://doi.org/10.1007/s00203-021-02264-z
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DOI: https://doi.org/10.1007/s00203-021-02264-z