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Chryseobacterium yeoncheonense sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field

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Abstract

A Gram-staining negative, aerobic, non-motile, non-flagellate, yellow-pigmented, rod-shaped bacterial strain, designated strain DCY67T, was isolated from ginseng field in Republic of Korea. Strain DCY67T contained β-glucosidase activity which converts ginsenoside Rb1 to compound K. Optimum growth of DCY67T occurred at 30 °C and pH 6.0–6.5. Analysis of the 16S rRNA gene sequences revealed that strain DCY67T belonged to the family Flavobacteriaceae and was most closely related to Chryseobacterium ginsenosidimutans THG 15T (97.5 %). The genomic DNA G+C content was 36.1 mol%. The predominant quinones were MK-6 (90.9 %) and MK-7 (9.15 %). The major fatty acids were iso-C15:0, summed feature 3 (containing C16:1 ω7c and/or C16:1 ω6c) and iso-C17:0 3-OH. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY67T represents a novel species of the genus Chryseobacterium, for which, name Chryseobacterium yeoncheonense sp. nov. proposed the type strain is DCY67T (=KCTC 32090T = JCM 18516T).

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Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (#20110015122).

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Authors and Affiliations

  1. Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of Korea

    Van-An Hoang, Yeon-Ju Kim, Ngoc Lan Nguyen & Deok-Chun Yang

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  1. Van-An Hoang
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  2. Yeon-Ju Kim
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  3. Ngoc Lan Nguyen
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  4. Deok-Chun Yang
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Corresponding authors

Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY67T is JX141782.

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Hoang, VA., Kim, YJ., Nguyen, N.L. et al. Chryseobacterium yeoncheonense sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field. Arch Microbiol 195, 463–471 (2013). https://doi.org/10.1007/s00203-013-0898-2

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