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Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan

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Abstract

A bacterial strain designated antisso-27T, previously isolated from saltpan in Taiwan while screening for bacteria for algicidal activity, was characterized using the polyphasic taxonomic approach. Strain antisso-27T was Gram-negative, aerobic, brownish yellow colored, rod-shaped, non-flagellated and non-gliding. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain antisso-27T belonged to the genus Aquimarina within the family Flavobacteriaceae with relatively low sequence similarities of 94.0–96.6% to other valid Aquimarina spp. It contained iso-C17:0 3-OH, iso-C15:0, iso-C16:0, iso-C15:1 and iso-C15:0 3-OH as the main fatty acids and contained a menaquinone with six isoprene units (MK-6) as the major isoprenoid quinone. Major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, an uncharacterized aminolipid and five uncharacterized phospholipids. Strain antisso-27T employed direct mode of algicidal lysis to Chlorella vulgaris strain 211-31; nevertheless, it released an algicidal substance against M. aeruginosa strain MTY01. This is the first study that the Aquimarina species possesses both direct and indirect algicidal activities. On the basis of the phylogenetic and phenotypic data, strain antisso-27T should be classified as representing a novel species, for which the name A. salinaria sp. nov. is proposed. The type strain is A. salinaria antisso-27T (= BCRC 80080T = LMG 25375T).

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Acknowledgments

This work was supported by grants 0980055254D and 0990045921J from the Ministry of Education, Republic of China, Taiwan. The authors wish to thank Dr. Hung-Non Chou (Institute of Fisheries Science, Taiwan University, Taiwan) for providing M. aeruginosa strain MTY01 and Dr. Te-Jin Chow (Department of Biotechnology, Fooyin University, Taiwan) for providing C. vulgaris strain 211-31.

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City, 811, Taiwan

    Wen-Ming Chen

  2. Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City, 811, Taiwan

    Fu-Sian Sheu & Shih-Yi Sheu

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  1. Wen-Ming Chen
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  2. Fu-Sian Sheu
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Correspondence to Shih-Yi Sheu.

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Communicated by Erko Stackebrandt.

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Chen, WM., Sheu, FS. & Sheu, SY. Aquimarina salinaria sp. nov., a novel algicidal bacterium isolated from a saltpan. Arch Microbiol 194, 103–112 (2012). https://doi.org/10.1007/s00203-011-0730-9

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