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Colutea arborescens is nodulated by diverse rhizobia in Eastern Morocco

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Abstract

Eighteen isolates of rhizobia isolated from root nodules of Colutea arborescens (Bladder senna) grown in different soils of the eastern area of Morocco were characterized by phenotypic and genomic analyses. All the isolates characterized were fast growers. This is may be due to the isolation procedures used. The phenotypic, symbiotic and cultural characteristics analyzed allowed the description of a wide physiological diversity among tested isolates. The results obtained suggest that the phenotype of these rhizobia might have convergent evolved to adapt the local conditions. The genetic characterization consisted in an analysis of the rep-PCR fingerprints and the PCR-based RFLP of the 16S rDNA patterns. The 16S rDNA of six isolates representing the main ribotypes obtained by the PCR-based RFLP was sequenced. A large diversity was observed among these rhizobia, and they were classified as different species of the genera Rhizobium, Sinorhizobium and Mesorhizobium. The nodC gene was also sequenced, and the results confirmed the three lineages corresponding to the three genera. The results of the sequencing of nodC and 16S rDNA genes suggest that the nodulation genes and chromosome might have co-evolved among these bacteria.

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Authors and Affiliations

  1. Laboratoire de Biologie des Plantes et des Microorganismes, Faculty of Sciences, Mohamed Premier University, Oujda, Morocco

    Mohammed Ourarhi, Hanaa Abdelmoumen, Kamal Guerrouj, Hanane Benata & Mustapha Missbah El Idrissi

  2. C.N.R.—Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo (ISPAAM)-Sezione di Sassari, c\o Dipartimento di Scienze Agronomiche e Genetica Vegetale Agraria, Università degli Studi di Sassari, Via Enrico de Nicola, 07100, Sassari, Italy

    Rosella Muresu

  3. Dipartimento di Biotecnologie Agrarie, Universita` di Padova, Viale dell′Universita` 16, 35020, Legnaro (Padova), Italy

    Andrea Squartini

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  1. Mohammed Ourarhi
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  2. Hanaa Abdelmoumen
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  3. Kamal Guerrouj
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  4. Hanane Benata
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  5. Rosella Muresu
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  6. Andrea Squartini
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  7. Mustapha Missbah El Idrissi
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Correspondence to Mustapha Missbah El Idrissi.

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Communicated by Ursula Priefer.

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Ourarhi, M., Abdelmoumen, H., Guerrouj, K. et al. Colutea arborescens is nodulated by diverse rhizobia in Eastern Morocco. Arch Microbiol 193, 115–124 (2011). https://doi.org/10.1007/s00203-010-0650-0

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  • DOI: https://doi.org/10.1007/s00203-010-0650-0

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