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Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp. strain JS614

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Abstract

A virtually identical nicotine catabolic pathway including the heterotrimeric molybdenum enzyme nicotine and 6-hydroxy-pseudo-oxynicotine dehydrogenase, 6-hydroxy-l-nicotine oxidase, 2,6-dihydroxy-pseudo-oxynicotine hydrolase, and 2,6-dihydroxypyridine hydroxylase have been identified in A. nicotinovorans and Nocardioides sp. JS614. Enzymes catalyzing the same reactions and similar protein antigens were detected in the extracts of the two microorganisms. Nicotine blue and methylamine, two end products of nicotine catabolism were detected in the growth medium of both bacterial species. Nicotine catabolic genes are clustered on pAO1 in A. nicotinovorans, but located chromosomally in Nocardioides sp. JS614.

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Acknowledgments

We thank Mrs. Veronika Erichsen for excellent technical assistance. This work was supported by a grant from the Deutsche Forschungsgemeinschaft to RB.

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Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Biology, Albert-Ludwigs-University Freiburg, Hermann Herder Str. 7, 79104, Freiburg, Germany

    Petra Ganas, Paula Sachelaru & Roderich Brandsch

  2. Department of Experimental Biology, Alexandru-Ioan-Cuza-University Iasi, Bd. Carol I 20 A, 700505, Iasi, Romania

    Marius Mihasan

  3. Institute of Biology III, Albert-Ludwigs-University Freiburg, Schänzlestr. 1, 79104, Freiburg, Germany

    Gabor L. Igloi

Authors
  1. Petra Ganas
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  2. Paula Sachelaru
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  3. Marius Mihasan
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  4. Gabor L. Igloi
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  5. Roderich Brandsch
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Corresponding author

Correspondence to Roderich Brandsch.

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Communicated by Erko Stackebrandt.

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Ganas, P., Sachelaru, P., Mihasan, M. et al. Two closely related pathways of nicotine catabolism in Arthrobacter nicotinovorans and Nocardioides sp. strain JS614. Arch Microbiol 189, 511–517 (2008). https://doi.org/10.1007/s00203-007-0340-8

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  • DOI: https://doi.org/10.1007/s00203-007-0340-8

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