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Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061

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Abstract

The four overlapping cosmids from the rubradirin producer, Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS (rubA) and glycosyltransferase (rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.

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Abbreviations

AHBA:

3-Amino-5-hydroxybenzoic acid

AMC:

3-Amino-4-hyrdoxy-7-methoxycoumarin

DHDP:

3,4-Dihydroxydipicolinate

NRPS:

Nonribosomal polypeptide synthetase

PKS:

Polyketide synthase

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Acknowledgments

This study was supported by the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology (Grant MG02-0301-004-2-3-1), Republic of Korea. The standard rubradirin sample was supplemented by Pfizer Global Research and Development Strategic Alliances, Eastern Point Road, Gorton, Connecticut.

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, Inje University, Kimhae, Kyung-nam, 621-749, Republic of Korea

    Chun-Gyu Kim, Kwang-Il Song, Van D. Nguyen, Dong-Hee Kim, Tae-Sung Jeong, Sung-Ho Kang, Kyoung-Wook Kim & Jae Seon Kang

  2. Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Republic of Korea

    Janardan Lamichhane, Jyoti Maharjan, Tae-Jin Oh, Kwangkyoung Liou & Jae Kyung Sohng

  3. Korea Research Institute of Bioscience and Biotechnology, KIST, Daejeon, 305-600, Republic of Korea

    Young-Soo Hong & Jung-Joon Lee

  4. Department of Pharmacy, Chosun University, Kwangju, 501-759, Republic of Korea

    Jin-Cheol Yoo

  5. Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Republic of Korea

    Jae Kyung Sohng

Authors
  1. Chun-Gyu Kim
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  2. Janardan Lamichhane
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  3. Kwang-Il Song
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  4. Van D. Nguyen
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  5. Dong-Hee Kim
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  7. Sung-Ho Kang
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  8. Kyoung-Wook Kim
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  9. Jyoti Maharjan
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  10. Young-Soo Hong
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  11. Jae Seon Kang
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  12. Jin-Cheol Yoo
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  13. Jung-Joon Lee
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  14. Tae-Jin Oh
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  15. Kwangkyoung Liou
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  16. Jae Kyung Sohng
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Corresponding author

Correspondence to Jae Kyung Sohng.

Additional information

Communicated by Jean-Luc Pernodet.

The GeneBank accession number for the sequence reported in this paper is AJ871581.

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Kim, CG., Lamichhane, J., Song, KI. et al. Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. Arch Microbiol 189, 463–473 (2008). https://doi.org/10.1007/s00203-007-0337-3

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  • DOI: https://doi.org/10.1007/s00203-007-0337-3

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