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Functional Characterization of Ketoreductase (rubN6) and Aminotransferase (rubN4) Genes in the Gene Cluster of Streptomyces achromogenes var. rubradiris

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Abstract

ORF’s for rubN6 and rubN4 have been annotated as thymidine diphosphate glucose 4-ketoreductase and thymidine diphosphate glucose 3-aminotransferase by sequence analysis of the rubradirin biosynthetic gene cluster cloned from Streptomyces achromogenes var. rubradiris NRRL 3061. Both ORFs were heterologously expressed in Escherichia coli as His-tagged fusion proteins. The functionalities of TDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferase were verified by in vitro enzyme assay, and a biosynthetic pathway for TDP-d-rubranitrose is proposed.

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Authors and Affiliations

  1. Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University, Tangjeonmyun, 336-708, Asan-si, Chung-nam, Republic of Korea

    Janardan Lamichhane, Kwangkyoung Liou, Hei Chan Lee & Jae Kyung Sohng

  2. Department of Pharmaceutical Engineering, Inje University, 621-749, Kimhae, Kyung-nam, Republic of Korea

    Chun-Gyu Kim

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  1. Janardan Lamichhane
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  2. Kwangkyoung Liou
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  3. Hei Chan Lee
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  4. Chun-Gyu Kim
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  5. Jae Kyung Sohng
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Correspondence to Jae Kyung Sohng.

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Lamichhane, J., Liou, K., Lee, H.C. et al. Functional Characterization of Ketoreductase (rubN6) and Aminotransferase (rubN4) Genes in the Gene Cluster of Streptomyces achromogenes var. rubradiris. Biotechnol Lett 28, 545–553 (2006). https://doi.org/10.1007/s10529-006-0013-8

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  • DOI: https://doi.org/10.1007/s10529-006-0013-8

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