Abstract
The toxicity of high-chlorinated polychlorinated biphenyls (PCBs) can be efficiently reduced through anaerobic dechlorination. However, this approach suffers a lot in face of in situ microbial remediations, like a shortage of biomass. In this study, we showed that the amendment of organic matters could help microbiota in paddy soil with anaerobic dechlorination and greatly shortened the lag period. The presence of organic matters offered a better environment for dechlorinating bacteria. They provided not only a more strictly anaerobic milieu but also copious carbon sources. By using high-throughput 16S rRNA gene sequencing, genera Dehalobacter, Dehalobacterium, and Desulfitobacterium capable of dechlorination were identified in enriched cultures. Taken together, this study proved that extra organic matters can promote anaerobic dechlorination in paddy soil slurry microcosm systems, which provides new insights into the bioremediation of PCB-contaminated soils.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abraham W-R, Nogales B, Golyshin PN et al (2002) Polychlorinated biphenyl-degrading microbial communities in soils and sediments. Curr Opin Microbiol 5:246–253. https://doi.org/10.1016/S1369-5274(02)00323-5
Alder AC, Haggblom MM, Oppenheimer SR, Young LY (1993) Reductive dechlorination of polychlorinated biphenyls in anaerobic sediments. Environ Sci Technol 27:530–538. https://doi.org/10.1021/es00040a012
Baba D, Yasuta T, Yoshida N et al (2007) Anaerobic biodegradation of polychlorinated biphenyls by a microbial consortium originated from uncontaminated paddy soil. World J Microbiol Biotechnol 23:1627–1636. https://doi.org/10.1007/s11274-007-9409-4
Bedard DL, Ritalahti KM, Löffler FE (2007) The Dehalococcoides Population in Sediment-Free Mixed Cultures Metabolically Dechlorinates the Commercial Polychlorinated Biphenyl Mixture Aroclor 1260. Appl Environ Microbiol 73:2513–2521. https://doi.org/10.1128/AEM.02909-06
Beyer A, Biziuk M (2009) Environmental fate and global distribution of polychlorinated biphenyls. Rev Environ Contam Toxicol 201:137–158. https://doi.org/10.1007/978-1-4419-0032-6_5
Cervantes FJ, de Bok FAM, Duong-Dac T et al (2002) Reduction of humic substances by halorespiring, sulphate-reducing and methanogenic microorganisms. Environ Microbiol 4:51–57. https://doi.org/10.1046/j.1462-2920.2002.00258.x
Chen C, Yang K, Yu C et al (2014a) Influence of redox conditions on the microbial degradation of polychlorinated biphenyls in different niches of rice paddy fields. Soil Biol Biochem 78:307–315. https://doi.org/10.1016/j.soilbio.2014.08.014
Chen C, Yu C, Shen C et al (2014b) Paddy field – A natural sequential anaerobic–aerobic bioreactor for polychlorinated biphenyls transformation. Environ Pollut 190:43–50. https://doi.org/10.1016/j.envpol.2014.03.018
Cogliano VJ (1998) Assessing the cancer risk from environmental PCBs. Environ Health Perspect 106:317–323. https://doi.org/10.1289/ehp.98106317
Dolfing J, Harrison BK (1992) Gibbs free energy of formation of halogenated aromatic compounds and their potential role as electron acceptors in anaerobic environments. Environ Sci Technol 26:2213–2218. https://doi.org/10.1021/es00035a021
Field JA, Sierra-Alvarez R (2008) Microbial transformation and degradation of polychlorinated biphenyls. Environ Pollut 155:1–12. https://doi.org/10.1016/j.envpol.2007.10.016
Jahnke JC, Hornbuckle KC (2019) PCB Emissions from Paint Colorants. Environ Sci Technol 53:5187–5194. https://doi.org/10.1021/acs.est.9b01087
Jota MAT, Hassett JP (1991) Effects of environmental variables on binding of a PCB congener by dissolved humic substances. Environ Toxicol Chem 10:483–491. https://doi.org/10.1002/etc.5620100408
Matturro B, Ubaldi C, Rossetti S (2016) Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01502
Men Y, Seth EC, Yi S et al (2014) Sustainable Growth of Dehalococcoides mccartyi 195 by Corrinoid Salvaging and Remodeling in Defined Lactate-Fermenting Consortia. Appl Environ Microbiol 80:2133–2141. https://doi.org/10.1128/AEM.03477-13
Nies L, Vogel TM (1990) Effects of Organic Substrates on Dechlorination of Aroclor 1242 in Anaerobic Sediments. Appl Environ Microbiol 56:2612–2617. https://doi.org/10.1128/aem.56.9.2612-2617.1990
Oh K-H, Ostrofsky EB, Cho Y-C (2008) Molecular characterization of polychlorinated biphenyl-dechlorinating populations in contaminated sediments. J Microbiol 46:165–173. https://doi.org/10.1007/s12275-007-0214-4
Passatore L, Rossetti S, Juwarkar AA, Massacci A (2014) Phytoremediation and bioremediation of polychlorinated biphenyls (PCBs): State of knowledge and research perspectives. J Hazard Mater 278:189–202. https://doi.org/10.1016/j.jhazmat.2014.05.051
Rysavy JP, Yan T, Novak PJ (2005) Enrichment of anaerobic polychlorinated biphenyl dechlorinators from sediment with iron as a hydrogen source. Water Res 39:569–578. https://doi.org/10.1016/j.watres.2004.11.009
Sokol RC, Bethoney CM, Rhee G-Y (1994) Effect of hydrogen on the pathway and products of PCB dechlorination. Chemosphere 29:1735–1742. https://doi.org/10.1016/0045-6535(94)90319-0
Sun J, Pan L, Zhu L (2018) Formation of hydroxylated and methoxylated polychlorinated biphenyls by Bacillus subtilis: New insights into microbial metabolism. Sci Total Environ 613–614:54–61. https://doi.org/10.1016/j.scitotenv.2017.09.063
Wiegel J, Wu Q (2000) Microbial reductive dehalogenation of polychlorinated biphenyls. FEMS Microbiol Ecol 32:1–15. https://doi.org/10.1111/j.1574-6941.2000.tb00693.x
Xiang Y, Xing Z, Liu J et al (2020) Recent advances in the biodegradation of polychlorinated biphenyls. World J Microbiol Biotechnol 36:145. https://doi.org/10.1007/s11274-020-02922-2
Yan J, Ritalahti KM, Wagner DD, Löffler FE (2012) Unexpected Specificity of Interspecies Cobamide Transfer from Geobacter spp. to Organohalide-Respiring Dehalococcoides mccartyi Strains. Appl Environ Microbiol 78:6630–6636. https://doi.org/10.1128/AEM.01535-12
Yang Y, McCarty PL (1998) Competition for Hydrogen within a Chlorinated Solvent Dehalogenating Anaerobic Mixed Culture. Environ Sci Technol 32:3591–3597. https://doi.org/10.1021/es980363n
Zhu M, Yuan Y, Yin H et al (2022) Environmental contamination and human exposure of polychlorinated biphenyls (PCBs) in China: A review. Sci Total Environ 805:150270. https://doi.org/10.1016/j.scitotenv.2021.150270
Zhuang W-Q, Yi S, Feng X et al (2011) Selective Utilization of Exogenous Amino Acids by Dehalococcoides ethenogenes Strain 195 and Its Effects on Growth and Dechlorination Activity. Appl Environ Microbiol 77:7797–7803. https://doi.org/10.1128/AEM.05676-11
Zhuang W-Q, Yi S, Bill M et al (2014) Incomplete Wood-Ljungdahl pathway facilitates one-carbon metabolism in organohalide-respiring Dehalococcoides mccartyi. Proceedings of the National Academy of Sciences 111:6419–6424. https://doi.org/10.1073/pnas.1321542111
Acknowledgements
This work was supported by the National Natural Science Foundation of China (grant numbers 21876149, 42077125).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Jingwen Chen and Fengjun Xu contributed equally to this work.
Rights and permissions
About this article
Cite this article
Chen, J., Xu, F., Yang, K. et al. The amendment of Organic matters enhances the anaerobic dechlorination of Polychlorinated Biphenyls in Paddy Soil. Bull Environ Contam Toxicol 109, 393–400 (2022). https://doi.org/10.1007/s00128-022-03563-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-022-03563-x