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Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers

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Abstract

This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as ”stretch specific”, or it should be linked to the sampling site and characterised as ”site specific”. The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as ”site specific”. Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.

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References

  • Beliaeff B, Burgeot T (2002) Integrated biomarker response: a useful tool for ecological risk assessment. Environ Toxicol Chem 21(6):1316–1322

    Article  CAS  Google Scholar 

  • Bervoets L, Blust R (2003) Metal concentrations in water, sediment and gudgeon (Gobio gobio) from a pollution gradient: relationship with fish condition factor. Environ Pollut 126(1):9–19

    Article  CAS  Google Scholar 

  • Burgos-Aceves MA, Cohen A, Smith Y, Faggio C (2018) MicroRNAs and their role on fish oxidative stress during xenobiotic environmental exposures. Ecotoxicol Environ Saf 148:995–1000

    Article  CAS  Google Scholar 

  • Deutschmann B, Kolarević S, Brack W et al (2016) Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. Sci Total Environ 573:1441–1449

    Article  CAS  Google Scholar 

  • European Commission Regulation (2006) Setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union (No 1881/2006)

  • Fenech M (2000) The in vitro micronucleus technique. Mutat Res-Fund Mol M455(1–2):81–95

    Article  Google Scholar 

  • Fiorino E, Sehonova P, Plhalova L, Blahova J, Svobodova Z, Faggio C (2018) Effect of glyphosate on early life stages: comparison between Cyprinus carpio and Danio rerio. Environ Sci Pollut Res 25(9):8542–8854

    Article  CAS  Google Scholar 

  • Gajski G, Žegura B, Ladeira C, Pourrut B, Del Bo C, Novak M, Sramkova M, Milić M, Bjerve Gutzkow K, Costa S, Dusinka M, Brunborg G, Collins A (2019) The comet assay in animal models: from bugs to whales–(Part 1 Invertebrates). Mutat Res Rev Mutat Res 779:82–113

    Article  CAS  Google Scholar 

  • Jovanović J, Kolarević S, Milošković A et al (2018) Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. Sci Total Environ 621:1289–1299

    Article  CAS  Google Scholar 

  • Kangabam RD, Bhoominathan SD, Kanagaraj S, Govindaraju M (2017) Development of a water quality index (WQI) for the Loktak Lake in India. Appl Water Sci. https://doi.org/10.1007/s13201-017-0579-4

    Article  Google Scholar 

  • Kolarević S, Kračun-Kolarević M, Kostić J, Slobodnik J et al (2016) Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: the joint danube survey 3. Sci Total Environ 540:377–385

    Article  CAS  Google Scholar 

  • Kolarević S, Aborgiba M, Kračun-Kolarević M, Kostić J et al (2016) Evaluation of genotoxic pressure along the Sava River. PLoS ONE 11(9):e0162450

    Article  CAS  Google Scholar 

  • Kostić J, Kolarević S, Kračun-Kolarević M et al (2016) Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama). Environ Sci Pollut R 23(20):20783–20795

    Article  CAS  Google Scholar 

  • Milošković A, Dojčinović B, Kovačević S et al (2016) Spatial monitoring of heavy metals in the inland waters of Serbia: a multispecies approach based on commercial fish. Environ Sci Pollut Res 23(10):9918–9933

    Article  CAS  Google Scholar 

  • Official Gazette of RS (2011a) Regulation on the parameters of ecological and chemical status of surface water and parameters of the chemical and quantitative status of groundwater. No.74/2011

  • Official Gazette of RS (2011b) Regulation on quantity of pesticides, metals, metalloids, and other toxic substances, chemotherapeutics, anabolics, and other substances which can be found in food. Official Gazette of RS (No 28/2011)

  • Pagano M, Faggio C (2015) The use of erythrocyte fragility to assess xenobiotic cytotoxicity. Cell Biochem Funct 33:351–355

    Article  CAS  Google Scholar 

  • Squier MK, Cohen JJ (2001) Standard quantitative assays for apoptosis. Mol Biotechnol 19:305–312

    Article  CAS  Google Scholar 

  • Sunjog K, Gačić Z, Kolarević S et al (2012) Heavy metal accumulation and the genotoxicity in barbel (Barbus barbus) as indicators of the Danube River pollution. Sci World J 2012:351074

    Article  CAS  Google Scholar 

  • Williams WP (1965) The population density of four species of freshwater fish, roach (Rutilus rutilus (L.)), bleak (Alburnus alburnus (L.)), dace (Leuciscus leuciscus (L.)) and perch (Perca fluviatilis L.) in the River Thames at Reading. J Anim Ecol 34:173–185

    Article  Google Scholar 

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Acknowledgements

This study was founded by Ministry of Education, Science and Technological Development of Republic of Serbia Grant Nos. 451-03-68/2020-14/ 200178 and 451-03-68/2020-14/200007, the bilateral project of Republic of Serbia and Republic of Slovenia No. 30/2018-19, and the PhD scholarship funded by Ministry of Education, Science and Technological Development of Republic of Serbia No. 451-03-1709/2018-14/ev. No 2313 (scholarship holder: author Jovana Jovanović Marić). We would like to acknowledge the City Institute of Public Health, Belgrade, Serbia for annual datasets. The authors are grateful to Luka Gačić who provided improvements to our English.

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Authors and Affiliations

  1. Department for Hydroecology and Water protection, University of Belgrade, Institute for Biological Research ¨Siniša Stanković¨, National Institute of Republic of Serbia, Bulevar despota Stefana 142, Belgrade, 11000, Serbia

    Jovana M. Jovanović Marić, Margareta J. Kračun-Kolarević, Stoimir M. Kolarević, Momir M. Paunović & Petar B. Smiljanić

  2. Faculty of Biology, Chair of Microbiology, Center for Genotoxicology and Ecogenotoxicology, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia

    Jovana M. Jovanović Marić, Stoimir M. Kolarević & Branka S. Vuković-Gačić

  3. Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, Belgrade, 11000, Serbia

    Jelena Z. Đorđević, Jovana M. Kostić-Vuković, Karolina Z. Sunjog & Zoran M. Gačić

Authors
  1. Jovana M. Jovanović Marić
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  2. Margareta J. Kračun-Kolarević
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  3. Stoimir M. Kolarević
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  4. Jelena Z. Đorđević
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  5. Momir M. Paunović
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  6. Jovana M. Kostić-Vuković
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  7. Karolina Z. Sunjog
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  8. Petar B. Smiljanić
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  9. Zoran M. Gačić
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  10. Branka S. Vuković-Gačić
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Corresponding author

Correspondence to Margareta J. Kračun-Kolarević.

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Jovanović Marić, J.M., Kračun-Kolarević, M.J., Kolarević, S.M. et al. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. Bull Environ Contam Toxicol 105, 224–229 (2020). https://doi.org/10.1007/s00128-020-02944-4

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