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Pollutants Induced Oxidative Stress, DNA Damage and Cellular Deformities in Clarias gariepinus (Burchell), from River Yamuna in Delhi Region, India

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Abstract

The purpose of the present study was to evaluate the severity of pollution impact at two extreme sites of river Yamuna in Delhi region utilizing oxidative stress, genotoxic and histopathological biomarkers in gonad (ovary) of Clarias gariepinus. To evaluate oxidative stress, TBAR’s and ferric reducing antioxidant power (FRAP) assay were employed while comet assay and classic histology were used to estimate genotoxicity and cellular damage respectively. The results indicated significant increase (p < 0.001) in TBARs level (µmol/g wet tissue); significant decrease (p < 0.001) in FRAP value (U/mg tissue), significant increase (p < 0.001) in DNA damage and extensive abnormal histoarchitecture in ovarian samples procured from Okhla as compared to Wazirabad barrage. Thus, exposure to the increasing toxicity downstream the river is altering the activity of cellular total antioxidant capacity persuading oxidative stress and cellular damage, eventually distressing the heath of fish fauna directly and humans indirectly.

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Acknowledgements

The authors acknowledge the financial assistance provided by University Grant Commission (326752), New Delhi. The authors are thankful to the Chairperson, Department of Zoology, Kurukshetra University, Kurukshetra, India, for providing laboratory and library facilities.

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Authors and Affiliations

  1. Fish and Fisheries Laboratory, Kurukshetra University, Kurukshetra, Haryana, 136119, India

    Anil K. Tyor & Kanika Pahwa

  2. Department of Zoology, Kurukshetra University, Kurukshetra, India

    Anil K. Tyor & Kanika Pahwa

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  1. Anil K. Tyor
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  2. Kanika Pahwa
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Correspondence to Anil K. Tyor.

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The authors declare that they have no conflicts of interests.

Ethical Approval

Test organism was opened in humane manner approved in accordance with the Guide to the Care and Use of Experimental Animals by the Animal Care Committee of Kurukshetra University, Kurukshetra.

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Tyor, A.K., Pahwa, K. Pollutants Induced Oxidative Stress, DNA Damage and Cellular Deformities in Clarias gariepinus (Burchell), from River Yamuna in Delhi Region, India. Bull Environ Contam Toxicol 99, 33–38 (2017). https://doi.org/10.1007/s00128-017-2112-8

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