Abstract
Aims/hypothesis
Low birthweight is associated with a high risk of diabetes, but there are no reports discussing birthweight and pancreatic tissues in humans. The purpose of this study was to examine the correlation between birthweight and beta and alpha cell mass in humans.
Methods
Sixty-four Japanese adults with and without diabetes who underwent pancreatectomy and were able to recall their weight history including birthweight were included. Pancreatic tissues were stained for insulin and glucagon, and fractional beta cell area (BCA) and alpha cell area (ACA) were quantified. Islet size and density and beta cell replication were also quantified and their associations with birthweight were evaluated.
Results
In participants without diabetes, there was a weak positive correlation between birthweight and BCA (R = 0.34, p = 0.03). The group with a history of childhood obesity, but not the group with a history of obesity in adulthood only, showed higher BCA compared with those without a history of obesity (1.78 ± 0.74% vs 0.99 ± 0.53%, p = 0.01), and the correlation coefficient between birthweight and BCA increased after excluding those with a history of childhood obesity (R = 0.51, p < 0.01). In those with diabetes, there was no correlation between birthweight and BCA. No correlation was found between birthweight and ACA in either those with or without diabetes.
Conclusions/interpretation
Birthweight and beta, but not alpha, cell mass are positively correlated in non-diabetic adults, and a history of childhood obesity may affect beta cell mass.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
It has been reported that beta cell mass (BCM) decreases not only in type 1 diabetes but also type 2 diabetes [1,2,3,4]. Because the prevalence of type 2 diabetes is increasing worldwide, it is important to develop a strategy to preserve and restore BCM.
Normally, the decrease in insulin sensitivity induced by obesity is compensated for by increasing insulin secretion to maintain normal glucose tolerance [5]. However, it has been suggested that, in contrast to rodents, the increase in BCM in the presence of obesity is relatively small in humans. In the Europid population, it has been reported that BCM increases by approximately 20–50% in obese individuals without diabetes [6, 7], while we have previously reported that no significant increase in BCM occurs in obese non-diabetic Japanese adults [3, 8]. We have also shown that in non-diabetic Japanese individuals, corticosteroid administration, which induces insulin resistance, did not increase BCM [9], suggesting that beta cell regenerative capacity is very limited especially in this population. However, these studies have evaluated obesity and/or insulin resistance only in adulthood, when beta cell replication ability has decreased [6, 8, 10, 11]. In view of the wide inter-individual variation of BCM even in the non-diabetic population, exploring the factors that affect BCM will have a significant impact on prevention and treatment strategies in diabetes.
In 1991, Hales et al. showed a negative correlation between birthweight and the onset of type 2 diabetes [12]. Subsequently, the Developmental Origins of Health and Disease (DOHaD) hypothesis has been proposed: that the risk of developing adult-onset non-communicable diseases, such as type 2 diabetes, hypertension, dyslipidaemia, ischaemic heart disease and kidney disease is affected by fetal development [13, 14]. Although the underlying mechanism of the DOHaD hypothesis has not been fully elucidated, it has been recently reported that birthweight correlates with glomerular number in the kidney [15], suggesting that birthweight affects organ development, which leads to susceptibility to the future development of diseases. However, there are no reports examining the relation between birthweight and pancreatic tissues in humans.
Therefore, in this study, to elucidate the mechanism of the association between low birthweight and future risk of type 2 diabetes, we sought to address the following questions by using pancreatic tissues resected by surgery: (1) Is there any correlation between birthweight and BCM? (2) Is there any correlation between birthweight and alpha cell mass (ACM)? (3) Does a history of childhood obesity affect them?
Methods
Participants
This study was approved by the Ethics Committee of Keio University School of Medicine. From May 2012 to March 2019, 401 patients underwent pancreatectomy at Keio University Hospital. Of those, we included 64 Japanese patients with and without diabetes who could recall their weight history including birthweight (43 men and 21 women) in this study. The inclusion criteria were: (1) ability to recall their weight history in childhood and birthweight to at least 100 g; (2) gave written informed consent; and (3) the pancreatic tissue contained adequate amounts of normal pancreas for histological analysis. The exclusion criteria were: (1) type 1 diabetes; or (2) a functional neuroendocrine tumour such as insulinoma or glucagonoma. All of the patients with diabetes had been diagnosed with type 2 diabetes before the diagnosis of pancreatic disease (mean duration of diabetes 8.8 ± 5.9 years, Table 1). Some of the participants included in this study (n = 11) have also been included in our prior study [3].
Measurements and questionnaire
We obtained information about the details of pancreatic diseases, surgical procedures, body height and weight at the time of the operation from medical records. HbA1c, glycated albumin and plasma glucose levels were extracted from patients’ blood test data. HbA1c was measured by HPLC (HLC723G11, Tosoh, Tokyo, Japan) and expressed as National Glycohemoglobin Standardization Program value (%) and International Federation of Clinical Chemistry value (mmol/mol). Glycated albumin was measured by an enzymatic method (Lucica GA-L, Sekisui Medical, Tokyo, Japan).
The participants were asked to answer a questionnaire about their weight history and family history of diabetes. The detailed content of the questionnaire was as follows: (1) birthweight; (2) childhood and adolescent (i.e. early childhood, elementary school age, junior high school age and high school age) weight trajectories divided into five categories (i.e. very thin, thin, normal, fat, very fat); (3) body weight at the age of 20 and every decade thereafter; (4) maximum body weight in life; and (5) first- and second-degree family history of diabetes. We defined those who selected ‘fat’ or ‘very fat’ at least once in their childhood and adolescent weight trajectories as having a history of childhood obesity. All participants reached their maximum body weight after 20 years. Adulthood obesity was defined as current BMI of 25 kg/m2 or greater [16], and patients whose maximum BMI was 25 kg/m2 or greater were defined as having a history of adulthood obesity.
Pancreatic tissue processing
Pancreatic tissue resected at operation was immediately fixed in formaldehyde and embedded in paraffin for subsequent analysis. Of every four patients who underwent total pancreatectomy, pancreatic head tissues were sampled from two individuals and pancreatic body and tail tissues were sampled from the other two individuals, because of differences in surgical method. Then, 5 μm sections were cut from the tumour-free region and stained for light microscopy as follows: (1) with haematoxylin–eosin; (2) for insulin (peroxidase staining) with haematoxylin; (3) for glucagon with haematoxylin; and (4) for insulin and Ki67 for assessment of beta cell replication, as previously described [8, 9] (ESM Table 1). Antigen retrieval for Ki67 staining was carried out by heat treatment at 120°C in 0.01 mol/l citrate buffer pH 6.0 using an autoclave instrument for 20 min and cooled down to room temperature (RT) and moved into PBS. Quenching of endogenous peroxidase was performed in 0.3% H2O2 in methanol, for 10 min at RT and rinsed with distilled water and then washed three times with PBS for 5 min. These slides were treated with primary antibodies for 3 h at RT and washed with PBS, then treated with peroxidase-conjugated secondary antibodies for 30 min at RT and rinsed with PBS. Color development was done using treatment with 3,3′-diaminobenzidine (DAB) or SG Peroxidase Substrate Kit SK4700 (Vector, Burlingame, CA, USA). Finally, haematoxylin or nuclear fast red counterstaining was performed.
Morphometric analysis
To quantify fractional beta cell area (BCA), a single cross-sectional pancreatic section for each participant was used. The entire pancreatic section containing ~300 islets (total pancreas area 126 ± 50 mm2) was imaged at the original magnification of ×200 (×20 objective) using a NanoZoomer-XR slide scanner and viewed with NDP.view2 software (Hamamatsu Photonics, Shizuoka, Japan), and the ratio of BCA to total pancreas area was digitally measured using Image Pro Premier software (Media Cybernetics, Silver Spring, MD, USA). Likewise, the ratio of alpha cell area (ACA) to total pancreas area was also digitally measured, and the ratio of ACA to BCA was determined in each case. All measurements were conducted by a single investigator (H. Sasaki); the inter-observer coefficient of variance was assessed between H. Sasaki and other colleagues in the laboratory and was approximately 11%; the intra-observer coefficient of variance was approximately 5%. All measurements were conducted twice, and the mean of the two measurements was used. At the time of the measurement, the investigator was blinded to birthweight, BMI and the glucose metabolism status for each specimen.
To conduct further morphometric analysis, mean islet size and islet density were quantified in randomly selected areas of the pancreas that contained at least 100 islets in each case (105 ± 5 islets, total 6741 islets, ESM Table 2) using NDP.view2 [8, 9]. Furthermore, as surrogate markers of beta cell turnover, scattered beta cells, insulin-positive duct cells and beta cell replication (i.e. by double staining with insulin and Ki67) were quantified. Since the frequency of beta cell apoptosis was very low, as observed in our prior reports [3, 8, 9], it was not evaluated in this study. Scattered beta cells were defined as a cluster of three or fewer beta cells in acinar tissue, and the density of scattered beta cells was determined as the number of scattered beta cells/pancreas area (/mm2). Insulin-positive duct cells were also counted and expressed as the number of insulin-positive duct cells/pancreas area (/mm2). Frequency of beta cell replication was expressed as the percentage of islets. Mean beta cell diameter was measured as a surrogate of beta cell size, as previously described [8].
Statistical analysis
Data are presented as mean ± SD in the text and tables unless otherwise indicated. Mann–Whitney U test was used to analyse the difference between two groups, and Spearman correlation coefficient was used to assess the correlation between two variables. All analyses were performed using SPSS (version 25; SPSS, IBM, Chicago, IL, USA). A value of p < 0.05 was taken as statistically significant.
Results
Participant characteristics
Characteristics of participants with (DM group, n = 26) and without diabetes (NDM group, n = 38) are shown in Table 1 and ESM Table 2. There was no significant difference in age between the two groups. In the DM group, HbA1c, glycated albumin and plasma glucose levels, and current and maximum BMI, were significantly higher than in the NDM group. In both groups, pancreatic cancer was the most common pancreatic disease and pancreatoduodenectomy was the most frequent operative procedure. Mean birthweight was 3026 ± 466 g, and there was no significant difference between the groups. In the NDM and DM groups, 47% and 92% of patients, respectively, had a history of obesity during their life, and 16% and 35%, respectively, had a history of childhood obesity.
Effects of diabetes on islet morphology
BCA was significantly smaller in the DM group compared with the NDM group (0.75 ± 0.34% vs 1.14 ± 0.58%, p < 0.01, Table 1, ESM Fig. 1). There was no difference in ACA between the two groups (0.22 ± 0.10% vs 0.25 ± 0.17%, p = 0.98). Thus, the ACA to BCA ratio in the DM group was higher than that in the NDM group (0.32 ± 0.14 vs 0.24 ± 0.12, p = 0.01).
Islet density was significantly reduced in the DM group compared with the NDM group (2.69 ± 1.07 vs 3.90 ± 1.96 /mm2, p < 0.01, ESM Fig. 1). Likewise, the DM group showed significantly smaller mean islet size than the NDM group (4505 ± 1459 vs 7052 ± 2663 μm2, p < 0.01).
Birthweight and beta and alpha cell mass
In the NDM group, there was a weak positive correlation between birthweight and BCA (R = 0.34, p = 0.03, Fig. 1), but no correlation between birthweight and ACA (R = −0.13, p = 0.43). Thus, a negative correlation was observed between birthweight and ACA to BCA ratio in the NDM group (R = −0.50, p < 0.01). However, in the DM group, there was no correlation between birthweight and BCA, ACA, or ACA to BCA ratio (Fig. 1).
The NDM and DM groups were further classified according to the mean birthweight (Fig. 1, ESM Table 3). In the NDM group, those with birthweight ≥3000 g showed greater BCA than those with birthweight <3000 g (1.29 ± 0.60% vs 0.98 ± 0.53%, p = 0.04), with no significant difference in individual beta cell size (ESM Fig. 2).
Birthweight and other islet morphology
In the NDM group, birthweight and islet density were not correlated (R = 0.13, p = 0.45, Fig. 2), while there was a positive correlation between birthweight and mean islet size (R = 0.46, p < 0.01). Neither islet density nor mean islet size and birthweight were correlated in the DM group (R = 0.13, p = 0.52 and R = 0.23, p = 0.25, respectively). The NDM group with birthweight ≥3000 g showed greater mean islet size (8441 ± 2692 vs 5510 ± 1595 μm2, p < 0.01), but not islet density, than the NDM group with birthweight <3000 g.
Regarding surrogate markers of beta cell turnover (ESM Fig. 2), a positive correlation was found between birthweight and density of insulin-positive duct cells in the NDM group (R = 0.39, p = 0.02, Fig. 3). The NDM group with birthweight ≥3000 g showed an increase in density of insulin-positive duct cells (0.11 ± 0.09 vs 0.07 ± 0.10 /mm2, p = 0.04) and frequency of beta cell replication (0.82 ± 1.09 vs 0.22 ± 0.53 /100 islets, p = 0.04) compared with the NDM group with birthweight <3000 g.
Effects of current and childhood obesity on islet morphology
There was no correlation between current BMI and islet morphology including BCA, ACA, ACA to BCA ratio, islet density and mean islet size in either the NDM or DM group (ESM Fig. 3). Similarly, maximum BMI and islet morphology were not correlated in either group (ESM Fig. 4).
To explore the effects of childhood obesity, the NDM group was divided into three categories, i.e. participants with a history of childhood obesity (NDM-CO group, n = 6), those with a history of obesity only in adulthood (NDM-AO group, n = 12) and those with no history of obesity (lean; NDM-LN group, n = 20) (Fig. 4, ESM Table 4). The NDM-CO group, but not the NDM-AO group, showed greater BCA than the NDM-LN group (1.78 ± 0.74% vs 0.99 ± 0.53%, p = 0.01). The NDM-CO, but not NDM-AO, group also showed greater mean islet size compared with the NDM-LN group (9759 ± 1716 vs 6586 ± 2259 μm2, p = 0.01). In the NDM-CO group, no correlation was observed between birthweight and BCA (R = −0.60, p = 0.21, ESM Fig. 5), and there was no difference in BCA (1.89 ± 0.40% vs 1.67 ± 1.08%, p = 0.51) or mean islet size (8919 ± 585 vs 10,599 ± 2215 μm2, p = 0.51) between participants with and without adulthood obesity (n = 3, respectively). Of note, there was no difference in birthweight among the groups (ESM Table 4).
By contrast, in the DM group, when similarly classified by history of obesity, no difference in islet morphology was observed among the three groups (ESM Fig. 6, ESM Table 5), although there were only two participants in the DM-LN group.
Correlation coefficient between birthweight and BCA was increased in participants without a history of childhood obesity
Finally, considering the possibility that a history of childhood obesity may affect BCA in the NDM group, we further analysed the association between birthweight and BCA after excluding the NDM-CO group (n = 32, Fig. 5). As a result, the positive correlation coefficient between birthweight and BCA was increased (R = 0.51, p < 0.01). The positive correlation coefficients between birthweight and mean islet size and the density of insulin-positive duct cells, and the negative correlation coefficient between birthweight and ACA to BCA ratio were also increased (R = 0.50, 0.45 and −0.53, respectively, all p < 0.01). However, there remained no correlation between birthweight and ACA, islet density, density of scattered beta cells or frequency of beta cell replication. The positive correlation between birthweight and BCA was observed in both sexes (ESM Fig. 7). These results were consistent when only the NDM-LN group was analysed (n = 20, ESM Fig. 8).
Discussion
In this study, we report that: (1) birthweight and BCA, but not ACA, were positively correlated in Japanese non-diabetic adults; (2) in these individuals, birthweight and mean islet size, but not islet density, were positively correlated; (3) these correlations were not observed in participants with type 2 diabetes; and (4) a history of childhood obesity, rather than adulthood obesity, may affect BCA and mean islet size.
Asians are thought to have a lower insulin secretion capacity compared with people of European or African descent [17]. In previous studies, including ours, neither current BMI nor maximum BMI in life were correlated with BCA in Japanese adults [3, 8, 18, 19]. The same results were also observed in this study, suggesting that Asians lack the capacity for beta cell proliferation to compensate for insulin resistance, compared with other ethnicities [1]. However, these previous studies only took into account obesity in adulthood.
Although there are conflicting results regarding the association between high birthweight and incidence of type 2 diabetes [20,21,22], infants with low birthweight have been consistently reported to have increased incidence of type 2 diabetes [12, 23,24,25,26,27,28,29,30,31], which has been recently proposed as part of the DOHaD hypothesis [13, 14]. Although there is complex gene–environment interaction and the mechanisms of the association between low birthweight and future development of type 2 diabetes remain uncertain, an involvement of epigenetic modification has been recently proposed [32, 33]. Recent evidence has suggested that fetal undernutrition causes epigenetic modifications including DNA methylation, histone modifications and microRNA interaction, inducing beta cell dysfunction and insulin resistance in the offspring [32, 33]. It has been shown that mice with low birthweight are susceptible to impaired glucose tolerance due to epigenetic control, which suppresses beta cell proliferation [34]. In this study, we found that birthweight and BCA were positively correlated in Japanese non-diabetic adults, indicating that birthweight is a major determinant of BCM not only in rodents but also in humans.
Obesity in adulthood is a well-known risk for the development of type 2 diabetes [35]. Although individuals with low birthweight are at risk of developing type 2 diabetes, it has been reported that those born with low birthweight and with obesity in adulthood showed the highest risk of type 2 diabetes compared with those with normal birthweight and without adulthood obesity [36, 37], suggesting a significant interaction between birthweight and adulthood obesity in the incidence of type 2 diabetes. Taken together, our findings suggest that smaller BCM in individuals born with low birthweight explains at least in part the relationship between low birthweight and high risk of future development of type 2 diabetes.
On the other hand, there was no correlation between birthweight and ACA in both non-diabetic and diabetic participants, suggesting that, unlike beta cells, alpha cells may be less susceptible to epigenetic effects of low birthweight. Nonetheless, a negative correlation was observed between birthweight and ACA to BCA ratio in non-diabetic participants, indicating that people born with low birthweight show a relative increase in ACM. Future studies will be needed to explore whether the likelihood of developing type 2 diabetes in infants with low birthweight is more attributable to a decrease in BCM or a relative increase in ACM.
The second finding of this study was clarification of the associations of birthweight with islet density and mean islet size. Analysis of fetal pancreatic tissues has shown that lobular organisation of the pancreas as well as increase in islet size is observed from early prenatal stages [38]. A previous report has shown that BCM and islet size increase from birth to 20 years of age, but density of islets decreases with age [10], suggesting that new beta cell formation mainly occurs with increasing islet size. Here we found a significant correlation between birthweight and mean islet size, but not islet density, in non-diabetic individuals without a history of childhood obesity, suggesting that insufficient development of each islet rather than reduced number of islets is the mechanism of reduced BCM in people born with low birthweight.
Islet development involves beta cell replication, which is highest in neonates up to infancy and decreases with age [10, 11]. In this study, although beta cell replication was very rarely observed in adult humans, the frequency of beta cell replication was significantly lower in those with birthweight <3000 g than in those with birthweight ≥3000 g, suggesting the involvement of beta cell replication in greater islet size. Another source of beta cells may be insulin-positive duct cells, whose number was positively correlated with birthweight in this study. Although beta cell neogenesis and proliferation are active in the neonate, duct cells are assumed to have the capacity to convert to beta cells even in adulthood [39, 40]. Taken together, our findings suggest the possibility that the ability of beta cell production declines during the life course in people born with low birthweight, also highlighting the role of epigenetic modification, which is relatively stably transmitted until adulthood.
The third finding was that no correlation between birthweight and BCA or mean islet size was observed in participants with type 2 diabetes. Previous reports have shown that BCM declines in type 2 diabetes [1,2,3,4]. However, it is not yet known whether the decline in BCM in individuals with type 2 diabetes is a cause or consequence of the disease in humans. Our findings indicate that the presence of type 2 diabetes modifies the relationship between birthweight and BCM, and therefore birthweight is no longer a major determinant of BCM in individuals with type 2 diabetes.
Finally, we confirmed the importance of childhood obesity as a factor affecting islet morphology. Intriguingly, compared with non-diabetic participants who did not have a history of childhood obesity, regardless of the presence or absence of adulthood obesity, those with a history of childhood obesity showed significantly larger BCA and mean islet size despite there being no significant difference in birthweight. Furthermore, the correlation coefficients between birthweight and BCA, mean islet size, and density of insulin-positive duct cells in non-diabetic participants were further increased by excluding those with childhood obesity. Although the number of individuals with childhood obesity was small in this study, these results suggest that a history of childhood obesity is a modifier of BCM and islet size. It has been shown that elevated BMI only in adulthood rather than in adolescence was associated with development of diabetes [41]. Another study of Japanese female nurses has also reported an inverse relationship between BMI at age 18 and the incidence of diabetes [42]. Furthermore, adolescent low BMI is associated with an increase in the incidence of gestational diabetes [43, 44]. The inverse relationship between childhood obesity and diabetes may be in part explained by the capacity of beta cell expansion during this age, although it should be noted that an increase in body weight during childhood remains correlated with an increased risk of type 2 diabetes, especially in those with low birthweight [45]. In addition, whether the poor response of BCM to obesity in Japanese is attributable to the lower incidence of childhood obesity in this population [46, 47] is an intriguing question that needs further clarification.
A limitation of this study is that birthweight and history of obesity were based on a questionnaire. The average age of participants in this study was over 60 years, and we were not able to obtain documented records in childhood. However, previous studies with respect to birthweight also used questionnaires to collect information [25,26,27, 36, 37, 42, 43]. Moreover, we sought to maintain the reliability of the data by including only those who were able to recall their birthweight on a scale of at least every 100 g. Second, different portions of the pancreas were sampled according to the operative procedure. However, the proportion of endocrine cells has been shown to be relatively consistent regardless of the pancreatic site, except for the ventral portion of the pancreatic head [4]. Furthermore, the results were not changed when we analysed the cases of pancreatic head and body/tail separately (ESM Fig. 9). Third, pancreatic diseases might affect islet morphology; however, the results did not change depending on whether or not the primary disease was pancreatic cancer in this study (ESM Fig. 10). In addition, in this study, because of the presence of pancreatic diseases we were not able to measure pancreas weight or volume, and therefore actual BCM, the product of BCA and pancreas weight, was undetermined, although BCA is widely used as a surrogate for BCM. Thus, difference in pancreas weight might affect our findings, as reduced pancreas weight has been reported in animal models of intrauterine growth retardation [48]. Nonetheless, these limitations and biases described above should make the correlations or differences between the groups tend towards zero. Fourth, since all participants of this study were Japanese, our findings may not be applicable to other ethnicities. Finally, in our participants, there were only seven participants with birthweight <2500 g, which is generally considered as low birthweight [21], furthermore, owing to the nature of the cross-sectional design of the study, it is unclear whether those with low BCM would develop type 2 diabetes in the future. Our findings should be confirmed by future studies including a larger sample size and multiple ethnicities.
In conclusion, there was a positive correlation between birthweight and BCM, and birthweight and mean islet size in Japanese non-diabetic adults. ACM and islet density were not related to birthweight. A history of childhood obesity and the presence of type 2 diabetes may be factors affecting BCM after birth. These findings will be important bases for explaining the DOHaD hypothesis.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ACA:
-
Alpha cell area
- ACM:
-
Alpha cell mass
- BCA:
-
Beta cell area
- BCM:
-
Beta cell mass
- DM (group):
-
Participant group with diabetes
- DOHaD:
-
Developmental Origins of Health and Disease
- NDM (group):
-
Participant group without diabetes
- NDM-AO (group):
-
NDM group with history of obesity only in adulthood
- NDM-CO (group):
-
NDM group with history of childhood obesity
- NDM-LN (group):
-
NDM group with no history of obesity (lean)
References
Saisho Y (2015) Beta cell dysfunction: its critical role in prevention and management of type 2 diabetes. World J Diabetes 6(1):109–124. https://doi.org/10.4239/wjd.v6.i1.109
Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC (2003) β-Cell deficit and increased β-cell apoptosis in humans with type 2 diabetes. Diabetes 52(1):102–110. https://doi.org/10.2337/diabetes.52.1.102
Inaishi J, Saisho Y, Sato S et al (2016) Effects of obesity and diabetes on α- and β-cell mass in surgically resected human pancreas. J Clin Endocrinol Metab 101(7):2874–2882. https://doi.org/10.1210/jc.2016-1374
Sakuraba H, Mizukami H, Yagihashi N, Wada R, Hanyu C, Yagihashi S (2002) Reduced beta-cell mass and expression of oxidative stress-related DNA damage in the islet of Japanese type II diabetic patients. Diabetologia 45(1):85–96. https://doi.org/10.1007/s125-002-8248-z
Polonsky KS, Given BD, Van Cauter E (1988) Twenty-four-hour profiles and pulsatile patterns of insulin secretion in normal and obese subjects. J Clin Invest 81(2):442–448. https://doi.org/10.1172/jci113339
Saisho Y, Butler AE, Manesso E, Elashoff D, Rizza RA, Butler PC (2013) β-Cell mass and turnover in humans: effects of obesity and aging. Diabetes Care 36(1):111–117. https://doi.org/10.2337/dc12-0421
Rahier J, Guiot Y, Goebbels RM, Sempoux C, Henquin JC (2008) Pancreatic β-cell mass in European subjects with type 2 diabetes. Diabetes Obes Metab 10(Suppl 4):32–42. https://doi.org/10.1111/j.1463-1326.2008.00969.x
Kou K, Saisho Y, Satoh S, Yamada T, Itoh H (2013) Change in β-cell mass in Japanese nondiabetic obese individuals. J Clin Endocrinol Metab 98(9):3724–3730. https://doi.org/10.1210/jc.2013-1373
Sato S, Saisho Y, Inaishi J et al (2015) Effects of glucocorticoid treatment on β- and α-cell mass in Japanese adults with and without diabetes. Diabetes 64(8):2915–2927. https://doi.org/10.2337/db15-0151
Meier JJ, Butler AE, Saisho Y et al (2008) Beta-cell replication is the primary mechanism subserving the postnatal expansion of β-cell mass in humans. Diabetes 57(6):1584–1594. https://doi.org/10.2337/db07-1369
Gregg BE, Moore PC, Demozay D et al (2012) Formation of a human β-cell population within pancreatic islets is set early in life. J Clin Endocrinol Metab 97(9):3197–3206. https://doi.org/10.1210/jc.2012-1206
Hales CN, Barker DJ, Clark PM et al (1991) Fetal and infant growth and impaired glucose tolerance at age 64. BMJ 303(6809):1019–1022. https://doi.org/10.1136/bmj.303.6809.1019
Gluckman PD, Hanson MA (2004) Living with the past: evolution, development, and patterns of disease. Science 305(5691):1733–1736. https://doi.org/10.1126/science.1095292
de Boo HA, Harding JE (2006) The developmental origins of adult disease (Barker) hypothesis. Aust N Z J Obstet Gynaecol 46(1):4–14. https://doi.org/10.1111/j.1479-828X.2006.00506.x
Manalich R, Reyes L, Herrera M, Melendi C, Fundora I (2000) Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study. Kidney Int 58(2):770–773. https://doi.org/10.1046/j.1523-1755.2000.00225.x
Kanazawa M, Yoshiike N, Osaka T, Numba Y, Zimmet P, Inoue S (2005) Criteria and classification of obesity in Japan and Asia-Oceania. World Rev Nutr Diet 94:1–12. https://doi.org/10.1159/000088200
Kodama K, Tojjar D, Yamada S, Toda K, Patel CJ, Butte AJ (2013) Ethnic differences in the relationship between insulin sensitivity and insulin response: a systematic review and meta-analysis. Diabetes Care 36(6):1789–1796. https://doi.org/10.2337/dc12-1235
Mizukami H, Takahashi K, Inaba W et al (2014) Age-associated changes of islet endocrine cells and the effects of body mass index in Japanese. J Diabetes Investig 5(1):38–47. https://doi.org/10.1111/jdi.12118
Fujita Y, Kozawa J, Iwahashi H et al (2015) Increment of serum C-peptide measured by glucagon test closely correlates with human relative beta-cell area. Endocr J 62(4):329–337. https://doi.org/10.1507/endocrj.EJ14-0456
Harder T, Rodekamp E, Schellong K, Dudenhausen JW, Plagemann A (2007) Birth weight and subsequent risk of type 2 diabetes: a meta-analysis. Am J Epidemiol 165(8):849–857. https://doi.org/10.1093/aje/kwk071
Zhao H, Song A, Zhang Y, Zhen Y, Song G, Ma H (2018) The association between birth weight and the risk of type 2 diabetes mellitus: a systematic review and meta-analysis. Endocr J 65(9):923–933. https://doi.org/10.1507/endocrj.EJ18-0072
Tian G, Guo C, Li Q et al (2019) Birth weight and risk of type 2 diabetes: a dose–response meta-analysis of cohort studies. Diabetes Metab Res Rev 35(5):e3144. https://doi.org/10.1002/dmrr.3144
Barker DJ, Hales CN, Fall CH, Osmond C, Phipps K, Clark PM (1993) Type 2 (non-insulin-dependent) diabetes mellitus, hypertension and hyperlipidaemia (syndrome X): relation to reduced fetal growth. Diabetologia 36(1):62–67. https://doi.org/10.1007/bf00399095
Lithell HO, McKeigue PM, Berglund L, Mohsen R, Lithell UB, Leon DA (1996) Relation of size at birth to non-insulin dependent diabetes and insulin concentrations in men aged 50-60 years. BMJ 312(7028):406–410. https://doi.org/10.1136/bmj.312.7028.406
Curhan GC, Willett WC, Rimm EB, Spiegelman D, Ascherio AL, Stampfer MJ (1996) Birth weight and adult hypertension, diabetes mellitus, and obesity in US men. Circulation 94(12):3246–3250. https://doi.org/10.1161/01.cir.94.12.3246
Yarbrough DE, Barrett-Connor E, Kritz-Silverstein D, Wingard DL (1998) Birth weight, adult weight, and girth as predictors of the metabolic syndrome in postmenopausal women: the rancho Bernardo study. Diabetes Care 21(10):1652–1658. https://doi.org/10.2337/diacare.21.10.1652
Carlsson S, Persson PG, Alvarsson M et al (1999) Low birth weight, family history of diabetes, and glucose intolerance in Swedish middle-aged men. Diabetes Care 22(7):1043–1047. https://doi.org/10.2337/diacare.22.7.1043
Forsen T, Eriksson J, Tuomilehto J, Reunanen A, Osmond C, Barker D (2000) The fetal and childhood growth of persons who develop type 2 diabetes. Ann Intern Med 133(3):176–182. https://doi.org/10.7326/0003-4819-133-3-200008010-00008
Birgisdottir BE, Gunnarsdottir I, Thorsdottir I, Gudnason V, Benediktsson R (2002) Size at birth and glucose intolerance in a relatively genetically homogeneous, high-birth weight population. Am J Clin Nutr 76(2):399–403. https://doi.org/10.1093/ajcn/76.2.399
Eriksson JG, Forsen TJ, Osmond C, Barker DJ (2003) Pathways of infant and childhood growth that lead to type 2 diabetes. Diabetes Care 26(11):3006–3010. https://doi.org/10.2337/diacare.26.11.3006
Wadsworth M, Butterworth S, Marmot M, Ecob R, Hardy R (2005) Early growth and type 2 diabetes: evidence from the 1946 British birth cohort. Diabetologia 48(12):2505–2510. https://doi.org/10.1007/s00125-005-0007-4
Fernandez-Twinn DS, Hjort L, Novakovic B, Ozanne SE, Saffery R (2019) Intrauterine programming of obesity and type 2 diabetes. Diabetologia 62(10):1789–1801. https://doi.org/10.1007/s00125-019-4951-9
Zhu Z, Cao F, Li X (2019) Epigenetic programming and fetal metabolic programming. Front Endocrinol 10(764). https://doi.org/10.3389/fendo.2019.00764
Su Y, Jiang X, Li Y et al (2016) Maternal low protein isocaloric diet suppresses pancreatic β-cell proliferation in mouse offspring via miR-15b. Endocrinology 157(12):4782–4793. https://doi.org/10.1210/en.2016-1167
Guh DP, Zhang W, Bansback N, Amarsi Z, Birmingham CL, Anis AH (2009) The incidence of co-morbidities related to obesity and overweight: a systematic review and meta-analysis. BMC Public Health 9:88. https://doi.org/10.1186/1471-2458-9-88
Katanoda K, Noda M, Goto A, Mizunuma H, Lee JS, Hayashi K (2017) Impact of birth weight on adult-onset diabetes mellitus in relation to current body mass index: the Japan Nurses' Health Study. J Epidemiol 27(9):428–434. https://doi.org/10.1016/j.je.2016.08.016
Hu C, Mu Y, Wan Q et al (2020) Association between birth weight and diabetes: role of body mass index and lifestyle in later life. J Diabetes 12(1):10–20. https://doi.org/10.1111/1753-0407.12960
Meier JJ, Kohler CU, Alkhatib B et al (2010) Beta-cell development and turnover during prenatal life in humans. Eur J Endocrinol 162(3):559–568. https://doi.org/10.1530/eje-09-1053
Zare M, Rastegar S, Ebrahimi E, Roohipoor A, Shirali S (2017) Role of pancreatic duct cell in beta cell neogenesis: a mini review study. Diabetes Metab Syndr 11(Suppl 1):S1–S4. https://doi.org/10.1016/j.dsx.2016.08.005
Weir GC, Gaglia J, Bonner-Weir S (2020) Inadequate β-cell mass is essential for the pathogenesis of type 2 diabetes. Lancet Diabetes Endocrinol. https://doi.org/10.1016/S2213-8587(20)30022-X
Tirosh A, Shai I, Afek A et al (2011) Adolescent BMI trajectory and risk of diabetes versus coronary disease. N Engl J Med 364(14):1315–1325. https://doi.org/10.1056/NEJMoa1006992
Katanoda K, Noda M, Goto A, Mizunuma H, Lee JS, Hayashi K (2019) Being underweight in adolescence is independently associated with adult-onset diabetes among women: the Japan Nurses' health study. J Diabetes Investig 10(3):827–836. https://doi.org/10.1111/jdi.12947
Yeung EH, Hu FB, Solomon CG et al (2010) Life-course weight characteristics and the risk of gestational diabetes. Diabetologia 53(4):668–678. https://doi.org/10.1007/s00125-009-1634-y
Yachi Y, Tanaka Y, Nishibata I et al (2013) Low BMI at age 20 years predicts gestational diabetes independent of BMI in early pregnancy in Japan: Tanaka Women's clinic study. Diabet Med 30(1):70–73. https://doi.org/10.1111/j.1464-5491.2012.03712.x
Bhargava SK, Sachdev HS, Fall CH et al (2004) Relation of serial changes in childhood body-mass index to impaired glucose tolerance in young adulthood. N Engl J Med 350(9):865–875. https://doi.org/10.1056/NEJMoa035698
Wang Y, Lobstein T (2006) Worldwide trends in childhood overweight and obesity. Int J Pediatr Obes 1(1):11–25. https://doi.org/10.1080/17477160600586747
Anderson SE, Whitaker RC (2009) Prevalence of obesity among US preschool children in different racial and ethnic groups. Arch Pediatr Adolesc Med 163(4):344–348. https://doi.org/10.1001/archpediatrics.2009.18
Boehmer BH, Limesand SW, Rozance PJ (2017) The impact of IUGR on pancreatic islet development and β-cell function. J Endocrinol 235(2):R63–R76. https://doi.org/10.1530/joe-17-0076
Acknowledgements
We thank Y. Madokoro, Department of Pathology, Keio University School of Medicine, for technical assistance, W. Gray (London, UK) for editing the manuscript and Y. Tsuda (Davinci Medical Illustration Office, Tokyo, Japan) for editing the graphical abstract.
Funding
This study was supported by funding from the Japan Diabetes Foundation, Keio Gijuku Academic Development Funds, and a Grant-in-Aid for Scientific Research (18 K08488) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) (YS).
Author information
Authors and Affiliations
Contributions
All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data. They were responsible for drafting the article and revising it critically for important intellectual content. They approved the version to be published. YS is responsible for the integrity of the work as a whole.
Corresponding author
Ethics declarations
The authors declare that there are no relationships or activities that might bias, or be perceived to bias, their work.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
ESM
(PDF 1.64 mb)
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Sasaki, H., Saisho, Y., Inaishi, J. et al. Associations of birthweight and history of childhood obesity with beta cell mass in Japanese adults. Diabetologia 63, 1199–1210 (2020). https://doi.org/10.1007/s00125-020-05127-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00125-020-05127-2