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High-resolution genetic mapping of the pepper resistancelocus Bs3 governing recognition of the Xanthomonascampestris pv vesicatora AvrBs3 protein

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Abstract 

The pepper (Capsicum annuum) Bs3 gene confers resistance to Xanthomonas campestris pv vesicatoria strains expressing the avirulence protein AvrBs3. Using amplified fragment length polymorphism (AFLP) and bulked DNA templates from resistant and susceptible plants we identified markers linked to Bs3 and defined a 2.1-cM interval containing the target gene. Bs3-linked AFLP fragments were cloned and conformity of isolated PCR products with the desired markers was determined by hybridisation to membrane-bound AFLP reactions. AFLP markers flanking the target gene were converted into locus-specific PCR-based markers. These markers were employed for the analysis of 790 plants segregating for Bs3, resulting in a linkage map with a genetic resolution of 0.13 cM. Mapping of Bs3-linked markers in tomato placed them to a syntenic interval on tomato chromosome 2.

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Authors and Affiliations

  1. Centre National de la Recherche Scientifique, Institute des Sciences Végétales, Avenue de la Terrasse, 91190 Gif-sur-Yvette Cedex, France e-mail: bonas@genetik.uni-halle.de, Present addresses: L. Noël, U. Bonas, Institut für Genetik, Martin-Luther Universität, Weinbergweg 22, D-06099 Halle (Saale), Germany e-mail: lahaye@genetik.uni-halle.de, A. Ballvora, Max-Planck Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829 Köln, Germany, B.J. Veuskens, Institute of Molecular Cell Biology, University of Amsterdam, 1098 SM Amsterdam, The Netherlands, , , , , , FR

    M. Pierre, L. Noël, A. Ballvora, J. Veuskens & U. Bonas

  2. Institut für Genetik, Martin-Luther Universität, Weinbergweg 22, D-06099 Halle (Saale), Germany, , , , , , DE

    T. Lahaye

  3. Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany, , , , , , DE

    M. Ganal

Authors
  1. M. Pierre
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  2. L. Noël
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  3. T. Lahaye
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  4. A. Ballvora
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  5. J. Veuskens
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  6. M. Ganal
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  7. U. Bonas
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Additional information

Received: 15 October 1999 / Accepted: 29 November 1999

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Pierre, M., Noël, L., Lahaye, T. et al. High-resolution genetic mapping of the pepper resistancelocus Bs3 governing recognition of the Xanthomonascampestris pv vesicatora AvrBs3 protein. Theor Appl Genet 101, 255–263 (2000). https://doi.org/10.1007/s001220051477

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  • DOI: https://doi.org/10.1007/s001220051477

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