Abstract
Key message
Quantitative trait locus analysis identified independent novel loci in cucumbers responsible for resistance to races 0 and 1 of the anthracnose fungal pathogen Colletotrichum orbiculare.
Abstract
Cucumbers have been reported to be vulnerable to Colletotrichum orbiculare, causing anthracnose disease with significant yield loss under favorable conditions. The deployment of a single recessive Cssgr gene in cucumber breeding for anthracnose resistance was effective until a recent report on high-virulent strains infecting cucumbers in Japan conquering the resistance. QTL mapping was conducted to identify the resistance loci in the cucumber accession Ban Kyuri (G100) against C. orbiculare strains 104-T and CcM-1 of pathogenic races 0 and 1, respectively. A single dominant locus An5 was detected in the disease resistance hotspot on chromosome 5 for resistance to 104-T. Resistance to CcM-1 was governed by three loci with additive effects located on chromosomes 2 (An2) and 1 (An1.1 and An1.2). Molecular markers were developed based on variant calling between the corresponding QTL regions in the de novo assembly of the G100 genome and the publicly available cucumber genomes. Multiple backcrossed populations were deployed to fine-map An5 locus and narrow the region to approximately 222 kbp. Accumulation of An2 and An1.1 alleles displayed an adequate resistance to CcM-1 strain. This study provides functional molecular markers for pyramiding resistance loci that confer sufficient resistance against anthracnose in cucumbers.
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Data availability
The supporting data for this study are available in the Supplementary Material. The nucleotide sequence data reported herein are available from the DNA Data Bank of Japan (DDBJ) Sequence Read Archive. The other datasets generated in this study are available from the corresponding author upon request.
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Acknowledgements
We thank Z. Shuang, N. Nishi, and K. Takano of the University of Tsukuba for their technical assistance. Fungal strains and plant genetic resources were obtained from Genebank of the National Agriculture and Food Research Organization (NARO), Japan. This work was partly supported by the Ministry of Agriculture, Forestry and Fisheries (MAFF) of the Government of Japan commissioned project “A Collaborative Research Project on Characterization and Evaluation of Plant Genetic Resources for Food and Agriculture (PGRAsia)” (grant no. JPJ007117), the Japan Science and Technology Agency (Support for Pioneering Research Initiated by the Next Generation (SPRING); Grant number: JPMJSP2124), Grant-in-Aid for JSPS Fellows, JSPS KAKENHI Grant Number 23KJ1148.
Funding
This work was partly supported by the Ministry of Agriculture, Forestry, and Fisheries (MAFF) of the Government of Japan commissioned project ‘A Collaborative Research Project on Characterization and Evaluation of Plant Genetic Resources for Food and Agriculture (PGRAsia)’ (grant no. JPJ007117), the Japan Science and Technology Agency (Support for Pioneering Research Initiated by the Next Generation (SPRING); Grant number: JPMJSP2124), Grant-in-Aid for JSPS Fellows, JSPS KAKENHI Grant Number 23KJ1148.
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YY designed and supervised the study. FF and HM developed the populations and evaluated their phenotypes. SI and KS performed ddRAD sequencing. HM built a linkage map and performed QTL analysis. FF developed DNA markers and performed fine mapping and marker analysis. KN performed the sequencing and assembly of the G100 genome. FF and HM prepared the manuscript. All the authors reviewed the manuscript.
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Fitriyah, F., Matsuo, H., Isobe, S. et al. Different control of resistance to two Colletotrichum orbiculare pathogenic races 0 and 1 in cucumber (Cucumis sativus L.). Theor Appl Genet 137, 127 (2024). https://doi.org/10.1007/s00122-024-04633-w
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DOI: https://doi.org/10.1007/s00122-024-04633-w