Abstract
Key message
The new stripe rust resistance gene Yr4EL in tetraploid Th. elongatum was identified and transferred into common wheat via 4EL translocation lines.
Abstract
Tetraploid Thinopyrum elongatum is a valuable genetic resource for improving the resistance of wheat to diseases such as stripe rust, powdery mildew, and Fusarium head blight. We previously reported that chromosome 4E of the 4E (4D) substitution line carries all-stage stripe rust resistance genes. To optimize the utility of these genes in wheat breeding programs, we developed translocation lines by inducing chromosomal structural changes through 60Co-γ irradiation and developing monosomic substitution lines. In total, 53 plants with different 4E chromosomal structural changes were identified. Three homozygous translocation lines (T4DS·4EL, T5AL·4EL, and T3BL·4EL) and an addition translocation line (T5DS·4EL) were confirmed by the genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), FISH-painting, and wheat 55 K SNP array analyses. These four translocation lines, which contained chromosome arm 4EL, exhibited high stripe rust resistance. Thus, a resistance gene (tentatively named Yr4EL) was localized to the chromosome arm 4EL of tetraploid Th. elongatum. For the application of marker-assisted selection (MAS), 32 simple sequence repeat (SSR) markers were developed, showing specific amplification on the chromosome arm 4EL and co-segregation with Yr4EL. Furthermore, the 4DS·4EL line could be selected as a good pre-breeding line that better agronomic traits than other translocation lines. We transferred Yr4EL into three wheat cultivars SM482, CM42, and SM51, and their progenies were all resistant to stripe rust, which can be used in future wheat resistance breeding programs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bai B, Du JY, Lu QL, He CY, Zhang LJ, Zhou G, Xia XC, He ZH, Wang CS (2014) Effective resistance to wheat stripe rust in a region with high disease pressure. Plant Dis 98:891–897. https://doi.org/10.1094/PDIS-09-13-0909-RE
Bariana HS, Mcintosh RA (1994) Characterization and origin of rust and powdery mildew resistance genes in VPM1 wheat. Euphytica 76:53–61. https://doi.org/10.1007/BF00024020
Bi YF, Zhao QZ, Yan WK, Li MX, Liu YX, Cheng CY, Zhang L, Yu XQ, Li J, Qian CT, Wu YF, Chen JF, Lou QF (2020) Flexible chromosome painting based on multiplex PCR of oligonucleotides and its application for comparative chromosome analyses in Cucumis. Plant J 102:178–186. https://doi.org/10.1111/tpj.14600
Borrajo CI, Sánchez-Moreiras AM, Reigosa MJ (2022) Ecophysiological responses of tall wheatgrass germplasm to drought and salinity. Plants 11:1548. https://doi.org/10.3390/plants11121548
Carter AH, Chen XM, Garland-Campbell K, Kidwell KK (2009) Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise.’ Theor Appl Genet 119:1119–1128. https://doi.org/10.1007/s00122-009-1114-2
Ceoloni C, Forte P, Kuzmanović L, Tundo S, Moscetti I, Vita PD, Virili ME, D’Ovidio R (2017) Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL. Theor Appl Genet 130:2005–2024. https://doi.org/10.1007/s00122-017-2939-8
Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. J Indian Dent Assoc 27:314–337. https://doi.org/10.1080/07060660509507230
Chen XM (2013) High-temperature adult-plant resistance, key for sustainable control of stripe rust. Am J Plant Sci 4:608. https://doi.org/10.4236/ajps.2013.43080
Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ (1995a) Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet 91:1125–1128. https://doi.org/10.1007/BF00223930
Chen XM, Jones SS, Line RF (1995b) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee, and Lemhi and interactions of aneuploid wheats with races of Puccinia striiformis. Phytopathology 85:375–381. https://doi.org/10.1094/Phyto-85-375
Chen CJ, Chen H, Zhang Y, Thomas HR, Frank MH, He YH, Xia R (2020) TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant 13:1194–1202. https://doi.org/10.1016/j.molp.2020.06.009
Chen C, Han YS, Xiao H, Zou BC, Wu DD, Sha LN, Yang CR, Liu SQ, Cheng YR, Wang Y, Kang HY, Fan X, Zhou YH, Zhang T, Zhang HQ (2023) Chromosome-specific painting in Thinopyrum species using bulked oligonucleotides. Theor Appl Genet 136:177. https://doi.org/10.1007/s00122-023-04423-w
Dong N, Hu HY, Hu TZ, Li G, Li XJ, Chen XD, Zhang YJ, Ru ZG (2019) Detection and distribution of molecular markers for stripe rust resistance genes Yr5, Yr10 and Yr18 in 348 wheat germplasm. Acta Agric Boreal-Occident Sin 28:1960–1968
Duan (2018) Development of durum wheat-Thinopyrum elongatum disomic addition lines, substitution lines and translocation lines. Dissertation, Yangzhou University
Dvořák J, Chen KC (1984) Phylogenetic relationships between chromosomes of wheat and chromosome 2E of Elytrigia elongata. Can J Genet Cytol 26:128–132. https://doi.org/10.1139/g84-021
Dvořák J, Edge M, Ross K (1988) On the evolution of the adaptation of Lophopyrum elongatum to growth in saline environments. PNAS 85:3805–3809. https://doi.org/10.1073/PNAS85.11.3805
Fedak G (1999) Molecular aids for integration of alien chromatin through wide crosses. Genome 42:584–591. https://doi.org/10.1139/g99-046
Feng JY, Yao FJ, Wang MN, See DR, Chen XM (2023) Molecular mapping of Yr85 and comparison with other genes for resistance to stripe rust on wheat chromosome 1B. Plant Dis. https://doi.org/10.1094/PDIS-11-22-2600-RE
Gong BR, Zhang H, Yang YL, Zhang JW, Zhu W, Xu LL, Wang Y, Zeng J, Fan X, Sha LN, Zhang HQ, Wu DD, Chen GY, Zhou YH, Kang HY (2022) Development and identification of a novel wheat–Thinopyrum scirpeum 4E (4D) chromosomal substitution line with stripe rust and powdery mildew resistance. Plant Dis 106:975–983. https://doi.org/10.1094/PDIS-08-21-1599-RE
Gong BR, Zhao L, Zeng CY, Zhu W, Xu LL, Wu DD, Cheng YR, Wang Y, Zeng J, Fan X, Sha LN, Zhang HQ, Chen GY, Zhou YH, Kang HY (2023) Development and characterization of a novel wheat-tetraploid thinopyrum elongatum 6E (6D) disomic substitution line with stripe rust resistance at the adult stage. Plants 12:2311. https://doi.org/10.3390/plants12122311
Guo XR, Shi QH, Liu Y, Su HD, Zhang J, Wang M, Wang CH, Wang J, Zhang KB, Fu SL, Hu XJ, Jing DL, Wang Z, Li JB, Zhang PZ, Liu C, Han FP (2023) Systemic development of wheat-Thinopyrum elongatum translocation lines and their deployment in wheat breeding for Fusarium head blight resistance. Plant J. https://doi.org/10.1111/tpj.16190
Han FP, Lamb JC, James A (2006) High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. PNAS 103:3238–3243. https://doi.org/10.1073/PNAS0509650103
Han FP, Gao Z, Birchler JA (2009) Reactivation of an inactive centromere reveals epigenetic and structural components for centromere specification in maize. Plant Cell 21:1929–1939. https://doi.org/10.1105/tpc.109.066662
Han DJ, Wang QL, Zhang L, Wei GR, Zeng QD, Zhao J, Wang XJ, Huang LL, Kang ZS (2010) Evaluation of resistance of current wheat cultivars to stripe rust in northwest China, north China and the middle and lower reaches of Changjiang river epidemic area. Sci Agric Sin 43:2889–2896. https://doi.org/10.3864/j.issn.0578-1752.2010.14.007
Han YH, Zhang T, Thammapichai P, Weng YQ, Jiang JM (2015) Chromosome-specific painting in cucumis species using bulked oligonucleotides. Genetics 200:771–779. https://doi.org/10.1534/genetics.115.177642
Hao M, Zhang LQ, Zhao LB, Dai SF, Li AL, Yang WY, Xie D, Li QC, Ning SZ, Yan ZH, Wu BH, Lan XJ, Yuan ZW, Huang L, Wang JR, Zheng K, Chen WS, Yu M, Chen XJ, Chen MP, Wei YM, Zhang HG, Kishii M, Hawkesford MJ, Mao L, Zheng YL, Liu DC (2019) A breeding strategy targeting the secondary gene pool of bread wheat: introgression from a synthetic hexaploid wheat. Theor Appl Genet 132:2285–2294. https://doi.org/10.1007/s00122-019-03354-9
He Y, Feng LH, Jiang Y, Zhang LQ, Yan J, Zhao G, Wang JR, Chen GY, Wu BH, Liu DC, Huang L, Fahima T (2020) Distribution and nucleotide diversity of Yr15 in wild emmer populations and Chinese wheat germplasm. Pathogens 9:212. https://doi.org/10.3390/pathogens9030212
Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J (2003) PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci 43:1839–1847. https://doi.org/10.2135/cropsci2003.1839
Hou DY, Zhou XL, Wang LL, Wang WL, Ma DF, Jing JX (2010) Molecular mapping of high-temperature adult-plant stripe rust resistance gene Yrxy54-1 with RGAP markers. J Plant Prot 37:487–492
Hou LY, Jia JQ, Zhang XJ, Li X, Yang ZJ, Ma J, Guo HJ, Zhan HX, Qiao LY, Chang ZJ (2016) Molecular mapping of the stripe rust resistance gene Yr69 on wheat chromosome 2AS. Plant Dis 100:1717–1724. https://doi.org/10.1094/PDIS-05-15-0555-RE
Howell T, Hale I, Jankuloski L, Bonafede M, Gilbert M, Dubcovsky J (2014) Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status. Theor Appl Genet 127:2695–2709. https://doi.org/10.1007/s00122-014-2408-6
Huang S, Liu SJ, Zhang YB, Xie YZ, Wang XT, Jiao HX, Wu SS, Zeng QD, Wang QL, Singh RP, Bhavani S, Kang ZS, Wang CS, Han DJ, Wu JH (2021) Genome-wide wheat 55K SNP-based mapping of stripe rust resistance loci in wheat cultivar Shaannong 33 and their alleles frequencies in current Chinese wheat cultivars and breeding lines. Plant Dis 105:1048–1056. https://doi.org/10.1094/PDIS-07-20-1516-RE
Jan I, Saripalli G, Kumar K, Kumar A, Singh R, Batra R, Sharma PK, Balyan HS, Gupta PK (2021) Meta-QTLs and candidate genes for stripe rust resistance in wheat. Sci Rep 11:22923. https://doi.org/10.1038/s41598-021-02049-w
Jiang X, Wang Z, Feng J, Du ZY, Zhang ZJ, Zhang YB, Che MZ, Ren JD, Wang HG, Quan W (2023) Mapping and validation of a novel major QTL for resistance to stripe rust in four wheat populations derived from landrace Qishanmai. Front Plant Sci 14:1207764. https://doi.org/10.3389/fpls.2023.1207764
Jin HL, Zhang HP, Zhao XY, Long L, Guan FN, Wang YP, Huang LY, Zhang XY, Wang YQ, Li H, Li W, Pu ZE, Zhang YZ, Xu Q, Jiang QT, Wei YM, Ma J, Qi PF, Deng M, Kang HY, Zheng YL, Chen GY, Jiang YF (2023) Identification of a suppressor for the wheat stripe rust resistance gene Yr81 in Chinese wheat landrace Dahongpao. Theor Appl Genet 136:67. https://doi.org/10.1007/s00122-023-04347-5
Klymiuk V, Chawla HS, Wiebe K, Ens J, Fatiukha A, Govta L, Fahima T, Pozniak CJ (2022) Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing. Commun Biol 5:826. https://doi.org/10.1038/s42003-022-03773-3
Ko JM, Seo BB, Suh DY, Do GS, Park DS, Kwack YH (2002) Production of a new wheat line possessing the 1BL.1RS wheat-rye translocation derived from Korean rye cultivar Paldanghomil. Theor Appl Genet 104:171–176. https://doi.org/10.1007/s00122-001-0783-2
Komuro S, Endo R, Shikata K, Kato A (2013) Genomic and chromosomal distribution patterns of various repeated DNA sequences in wheat revealed by a fluorescence in situ hybridization procedure. Genome 56:131–137. https://doi.org/10.1139/gen-2013-0003
Li HW, Zheng Q, Pretorius ZA, Li B, Tang DZ, Li ZS (2016) Establishment and characterization of new wheat-Thinopyrum ponticum addition and translocation lines with resistance to Ug99 races. J Genet Genom 43:573–575. https://doi.org/10.1016/j.jgg.2016.07.004
Li DY, Long D, Li TH, Wu YL, Wang Y, Zeng J, Xu LL, Fan X, Sha LN, Zhang HQ, Zhou YH, Kang HY (2018a) Cytogenetics and stripe rust resistance of wheat-Thinopyrum elongatum hybrid derivatives. Mol Cytogenet 11:1–9. https://doi.org/10.1186/s13039-018-0366-4
Li DY, Li TH, Wu YL, Zhang XH, Zhu W, Wang Y, Zeng J, Xu LL, Fan X, Sha LN, Zhang HQ, Zhou YH, Kang HY (2018b) FISH-based markers enable identification of chromosomes derived from tetraploid Thinopyrum elongatum in hybrid lines. Front Plant Sci 9:526. https://doi.org/10.3389/fpls.2018.00526
Li DY, Zhang JW, Liu HJ, Tan BW, Zhu W, Xu LL, Wang Y, Zeng J, Fan X, Sha LN, Zhang HQ, Ma J, Chen GY, Zhou YH, Kang HY (2019) Characterization of a wheat-tetraploid Thinopyrum elongatum 1E (1D) substitution line K17-841-1 by cytological and phenotypic analysis and developed molecular markers. BMC Genom 20:1–16. https://doi.org/10.1186/s12864-019-6359-9
Li JB, Dundas I, Dong CM, Li GR, Trethowan R, Yang ZJ, Hoxha S, Zhang P (2020) Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. Theor Appl Genet 133:1095–1107. https://doi.org/10.1007/s00122-020-03534-y
Li YH, Wei ZZ, Sela H, Govta L, Klymiuk V, Roychowdhury R, Chawla HS, Ens J, Wiebe K, Bocharova V, Ben-David R, Pawar PB, Jaiwar S, Molnár I, Doležel J, Pozniak CJ, Fahima T (2022) Long-read genome sequencing accelerated the cloning of Pm69 by resolving the complexity of a rapidly evolving resistance gene cluster in wheat. bioRxiv. 10, 14.512294. https://doi.org/10.1101/2022.10.14.512294
Liao SM, Xu ZB, Fan XL, Zhou Q, Liu XF, Jiang C, Chen LE, Lin D, Feng B, Wang T (2023) Genetic dissection and validation of a major QTL for grain weight on chromosome 3B in bread wheat (Triticum aestivum L.). J Integr Agric. https://doi.org/10.1016/j.jia.2023.04.023
Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in north America, 1968–1987. US Dep Agric Tech Bull 1788:44
Liu LQ, Luo QL, Li HW, Li B, Li ZS, Zheng Q (2018) Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag and development of blue-grain-related molecular markers and a FISH probe based on SLAF-seq technology. Theor Appl Genet 131:2359–2370. https://doi.org/10.1007/s00122-018-3158-7
Liu D, Zhao DH, Zeng JQ, Shawai RS, Tong JY, Li M, Li FJ, Zhou S, Hu WL, Xia XC, Tian YB, Zhu Q, Wang CP, Wang DS, He ZH, Liu JD (2023) Identification of genetic loci for grain yield-related traits in the wheat population Zhongmai 578/Jimai 22. J Integr Agric 22:1985–1999. https://doi.org/10.1016/j.jia.2022.12.002
Liu AF (2007) Identification of tritielytrigia germplasm, the chromosomal location and SSR molecular marker of its resistant gene. Dissertation, Shandong Agricultural University.
Liu HJ (2022) Development of molecular markers specific for tetraploid Thinopyrum elongatum and their ultilization in breeding. Dissertation, Sichuan Agricultural University.
Lou HJ, Dong LL, Zhang KP, Wang DW, Zhao ML, Li YW, Rong CW, Qin HJ, Zhang AM, Dong ZY, Wang DW (2017) High-throughput mining of E-genome-specific SNP s for characterizing Thinopyrum elongatum introgressions in common wheat. Mol Ecol Resour 17:1318–1329. https://doi.org/10.1111/1755-0998.12659
Lu JL, Chen C, Liu P, He ZH, Xia XC (2016) Identification of a new stripe rust resistance gene in Chinese winter wheat Zhongmai 175. J Integr Agric 15:2461–2468. https://doi.org/10.1016/S2095-3119(16)61379-5
Luo PG, Ren ZL, Zhang HQ, Zhang HY (2005) Identification, chromosomal location, and diagnostic markers for a new gene (YrCN19) for resistance to wheat stripe rust. Phytopathology 95:1266–1270. https://doi.org/10.1094/PHYTO-95-1266
Ma JX, Zhou RH, Dong YC, Jia JZ (1999) Localization of a wheat stripe rust resistance gene from Lophopyrum elongatum. Chinese Science Bulletin 65–69.
McIntosh RA, Lagudah ES (2000) Cytogenetical studies in wheat. XVIII. Gene Yr24 for resistance to stripe rust. Plant Breed 119:81–93. https://doi.org/10.1046/j.1439-0523.2000.00449.x
Sharma-Poudyal D, Chen XM, Wan AM, Zhan GM, Kang ZS, Cao SQ, Jin SL, Morgounov A, Akin B, Mert Z, Shah SJA, Bux H, Ashraf M, Sharma RC, Madariaga R, Puri KD, Wellings C, Xi KQ, Wanyera R, Manninger K, Ganzalez MI, Koyda M, Sanin S, Patzek LJ (2013) Virulence characterization of international collections of the wheat stripe rust pathogen, Puccinia striiformis f. sp. tritici. Plant Dis 97:379–386. https://doi.org/10.1094/PDIS-01-12-0078-RE
Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516. https://doi.org/10.1139/g01-028
Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155. https://doi.org/10.2135/cropsci2000.4041148x
Sun Q, Wei Y, Ni Z, Xie C, Yang T (2002) Microsatellite marker for yellow rust resistance gene Yr5 in wheat introgressed from spelt wheat. Plant Breed 121:539–541. https://doi.org/10.1046/j.1439-0523.2002.00754.x
Tanaka H, Nabeuchi C, Kurogaki M, Garg M, Saito M, Ishikawa G, Nakamura T, Tsujimoto H (2017) A novel compensating wheat–Thinopyrum elongatum Robertsonian translocation line with a positive effect on flour quality. Breed Sci 67:509–517. https://doi.org/10.1270/jsbbs.17058
Tang ZX, Yang ZJ, Fu SL (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. J Appl Genet 55:313–318. https://doi.org/10.1007/s13353-014-0215-z
Wang HG, Ma DF, Tang S, Hou L, Jing JX (2013) Genetic analysis and molecular mapping of stripe rust resistance gene in wheat cultivar Xiaoyan 9366. J Plant Prot 40:418–424
Wang J, Liu YL, Su HD, Guo XG, Han FP (2017) Centromere structure and function analysis in wheat-rye translocation lines. Plant J 91:199–207. https://doi.org/10.1111/tpj.13554
Wang Y, Zhang HZ, Xie JZ, Guo BM, Chen YX, Zhang HY, Lu P, Wu QH, Li MM, Zhang DY, Guo GH, Yang J, Zhang PP, Zhang Y, Wang XC, Zhao H, Cao TJ, Liu ZY (2018) Mapping stripe rust resistance genes by BSR-Seq: YrMM58 and YrHY1 on chromosome 2AS In Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17. Crop J 6:91–98. https://doi.org/10.1016/j.cj.2017.03.002
Wang HW, Sun SL, Ge WY, Zhao LF, Hou BQ, Wang K, Lyu ZF, Chen LY, Xu SS, Guo J, Li M, Su PS, Li XF, Wang GP, Bo CY, Fang XJ, Zhuang WW, Cheng XX, Wu JW, Dong LH, Chen WY, Li W, Xiao GL, Zhao JX, Hao YC, Xu Y, Gao Y, Liu WJ, Liu YH, Yin HY, Li JZ, Li X, Zhao Y, Wang XQ, Ni F, Ma X, Li AF, Xu SS, Bai GH, Nevo E, Gao CX, Ohm H, Kong LR (2020) Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science 368:eaba5435. https://doi.org/10.1126/science.aba5435
Wang YQ, Yu C, Cheng YK, Yao FJ, Long L, Wu Y, Li J, Li H, Wang JR, Jiang QT, Li W, Pu ZE, Qi PF, Ma J, Deng M, Wei YM, Chen XM, Chen GY, Kang HY, Jiang YF, Zheng YL (2021) Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. BMC Genom 22:1–15. https://doi.org/10.1186/s12864-020-07331-1
Wellings CR (2011) Global status of stripe rust: a review of historical and current threats. Euphytica 179:129–141. https://doi.org/10.1007/s10681-011-0360-y
Wu L, Tan J, Zhu HZ, Wang ZL, Pu XR (2007) Detection of stripe rust resistant genes of Yr5, Yr10 and Yr15 in some Sichuan wheat lines by molecular markers. Southwest China J Agric Sci 20(2):316–320
Xing LP, Yuan L, Lv ZS, Wang Q, Yin CH, Huang ZP, Liu JQ, Cao SQ, Zhang RQ, Chen PD, Karafiátová M, Vrána J, Bartoš J, Doležel J, Cao AZ (2021) Long-range assembly of sequences helps to unravel the genome structure and small variation of the wheat-Haynaldia villosa translocated chromosome 6VS.6AL. Plant Biotechnol J 19:1567–1578. https://doi.org/10.1111/pbi.13570
Yang GT, Boshoff WHP, Li HW, Pretorius ZA, Luo QL, Li B, Li ZS, Zheng Q (2021) Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat-Thinopyrum ponticum translocation line WTT34. Theor Appl Genet 134:1587–1599. https://doi.org/10.1007/s00122-021-03796-0
Yang GT, Tong CY, Li HW, Li B, Li ZS, Zheng Q (2022) Cytogenetic identification and molecular marker development of a novel wheat-Thinopyrum ponticum translocation line with powdery mildew resistance. Theor Appl Genet 135:2041–2057. https://doi.org/10.1007/s00122-022-04092-1
Yin YH, Xu QX, Qi F, Bao YG, Wang HG, Li XF (2017) Screening and identification of wheat–Thinopyrum elongatum aline chromosomes lines. J Agric Biotechnol 25:689–699
Yuan CL, Jiang H, Wang HG, Li K, Tang H, Li XB, Fu DL (2012) Distribution, frequency and variation of stripe rust resistance loci Yr10, Lr34/Yr18 and Yr36 in Chinese wheat cultivars. J Genet Genom 39:587–592. https://doi.org/10.1016/j.jgg.2012.03.005
Zeng J, Zhou CL, He ZM, Wang Y, Xu LL, Chen GD, Zhu W, Zhou YH, Kang HY (2023) Disomic substitution of 3D chromosome with its homoeologue 3E in tetraploid Thinopyrum elongatum enhances wheat seedlings tolerance to salt stress. Int J Mol Sci 24:1609. https://doi.org/10.3390/ijms24021609
Zhang Z, Han HM, Liu WH, Song LQ, Zhang JP, Zhou SH, Yang XM, Li XQ, Li LH (2019) Deletion mapping and verification of an enhanced-grain number per spike locus from the 6PL chromosome arm of Agropyron cristatum in common wheat. Theor Appl Genet 132:2815–2827. https://doi.org/10.1007/s00122-019-03390-5
Zhang GS, Zhao YY, Kang ZS, Zhao J (2020) First report of a Puccinia striiformis f. sp. tritici race virulent to wheat stripe rust resistance gene Yr5 in China. Plant Dis 104:284. https://doi.org/10.1094/PDIS-05-19-0901-PDN
Zhou XL, Wang WL, Wang LL, Hou DY, Jing JX, Wang Y, Xu ZQ, Yao Q, Yin JL, Ma DF (2011) Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54. Theor Appl Genet 123:431–438. https://doi.org/10.1007/s00122-011-1595-7
Zhou SH, Zhang JP, Han HM, Zhang J, Ma HH, Zhang Z, Lu YQ, Liu WH, Yang XM, Li XQ, Li LH (2019) Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line. BMC Genom 20:1–15. https://doi.org/10.1186/s12864-019-6416-4
Zhou XW, Yue WY, Song JR, Cao SQ, Zhang YH, Liu HY, Wang N, Nan H, Zhao SW, Wei ZP (2017) Molecular detection of stripe rust resistant genes Yr5 and Yr10 in 38 wheat lines. Gansu Agr Sci and Techn 40–43.
Zhu C, Wang YZ, Chen CH, Wang CY, Ji WQ (2017) Molecular cytogenetic identification of Triticum aestivum-Thinopyrum ponticum 5J (E) disomic addition line with stripe rust resistance and salt tolerance. J Agric Biotechnol 25:689–699
Zhu ZW, Cao Q, Han DJ, Wu JH, Wu L, Tong JY, Xu XW, Yan J, Zhang Y, Xu KJ, Wang FJ, Dong YC, Gao CB, He ZH, Xia XC, Hao YF (2023) Molecular characterization and validation of adult-plant stripe rust resistance gene Yr86 in Chinese wheat cultivar Zhongmai 895. Theor Appl Genet 136:142. https://doi.org/10.1007/s00122-023-04374-2
Acknowledgements
This work was supported by the Major Program of National Agricultural Science and Technology of China (NK20220607), the National Natural Science Foundation of China (No. 31971883, No. 32200180), and the Science and Technology Bureau of Sichuan Province (2023NSFSC1995, 2022YFH0069, 2022NSFSC1671), and the Science and Technology Bureau of Chengdu City (2022-YF05-00449-SN). We thank Dr. George Fedak, Eastern Cereal and Oilseed Research Center, Ottawa, Canada, for kindly supplying the Trititrigia 8801 material used in this study. Thanks to Dr. Yinghui Li for editing and suggestion of the manuscript. We also thank Prof. Qiuzhen Jia, Plant Protection Institute of Gansu Academy of Agricultural Sciences, Gansu, China, for kindly supplying the urediniospores of the P. striiformis f. sp. tritici races.
Author information
Authors and Affiliations
Contributions
BRG, LFC, HZ, DDW, and HYK conducted the experiment, analyzed the data, and drafted the manuscript. WZ, LX, YRC, and YW developed substitution lines and evaluated disease resistance. JZ, XF, LNS, HQZ, GC, and YHZ provided technique guidance. DDW and HYK designed the experiment and formulated the questions. All the authors read and approved the manuscript.
Corresponding authors
Ethics declarations
Funding
The authors have not disclosed any funding.
Conflict of interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Data availability
The whole genome sequence of diploid Th. elongatum reported in this study can be found in the National Center for Biotechnology Information, NCBI BioProject ID PRJNA540081. Data supporting the results of this study are in the manuscript or supplemental file.
Additional information
Communicated by Lingrang Kong.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Gong, B., Chen, L., Zhang, H. et al. Development, identification, and utilization of wheat–tetraploid Thinopyrum elongatum 4EL translocation lines resistant to stripe rust. Theor Appl Genet 137, 17 (2024). https://doi.org/10.1007/s00122-023-04525-5
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00122-023-04525-5