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Fine mapping of BnDM1—the gene regulating indeterminate inflorescence in Brassica napus

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Abstract

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A candidate gene Bndm1 related to determinate inflorescence was mapped to a 128-kb interval on C02 in Brassica napus.

Abstract

Brassica napus plants with determinate inflorescence exhibit improved traits in field production, such as lower plant height, improved lodging resistance, and consistent maturity. Compared to plants with indeterminate inflorescence, such features are favorable for mechanized harvesting techniques. Here, using a natural mutant 6138 with determinate inflorescence, it is demonstrated that determinate inflorescence reduces plant height significantly without affecting thousand-grain weight and yield per plant. Determinacy was regulated by a single recessive gene, Bndm1. Using a combination of SNP arrays and map-based cloning, we mapped the locus of determinacy to a 128-kb region on C02. Based on sequence comparisons and the reported functions of candidate genes in this region, we predicted BnaC02.knu (a homolog of KNU in Arabidopsis) as a possible candidate gene of Bndm1 for controlling determinate inflorescence. We found a 623-bp deletion in a region upstream of the KNU promoter in the mutant. This deletion led to the significant overexpression of BnaC02.knu in the mutant compared to that in the ZS11 line. The correlation between this deletion and determinate inflorescence was examined in natural populations. The results indicated that the deletion affected the normal transcription of BnaC02.knu in the plants with determinate inflorescence and played an important role in maintaining flower development. This study presents as a new material for optimizing plant architecture and breeding novel canola varieties suitable for mechanized production. Moreover, our findings provide a theoretical basis for analyzing the molecular mechanisms underlying the formation of determinate inflorescence in B. napus.

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All data generated or analyzed during this study are included in this published article and its supplementary information files.

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Acknowledgements

This work was financed by the National Key Research and Development Program of China (Grant Number 2016YFD0100305).

Funding

This work was funded by the National Key Research and Development Program of China [Grant No. 2016YFD0100305].

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Jiao Chen, Sihao Zhang, Bao Li, Chenjian Zhuo, Kaining Hu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxiong Shen, Tingdong Fu & Jinxing Tu

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  1. Jiao Chen
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  2. Sihao Zhang
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  3. Bao Li
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  11. Jinxing Tu
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Contributions

JC performed the experiments and prepared the manuscript. SZ and BL helped with the experiments. CZ and KH helped analyze the data. JW, BY, CM, JS, and TF provided suggestions related to the experimental design and provided the experimental platform. JT oversaw the project and revised the manuscript.

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Correspondence to Jinxing Tu.

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Chen, J., Zhang, S., Li, B. et al. Fine mapping of BnDM1—the gene regulating indeterminate inflorescence in Brassica napus. Theor Appl Genet 136, 151 (2023). https://doi.org/10.1007/s00122-023-04384-0

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