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Identification of QTL for reducing loss of grain yield under salt stress conditions in bi-parental populations derived from wheat landrace Hongmangmai

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Abstract

Key message

A novel QTL (QSt.nftec-2BL) was mapped to a 0.7 cM interval on chromosome 2B. Plants carrying QSt.nftec-2BL produced higher grain yields by up to 21.4% than otherwise in salinized fields.

Abstract

Wheat yield has been limited by soil salinity in many wheat-growing areas globally. The wheat landrace Hongmangmai (HMM) possesses salt tolerance as it produced higher grain yields than other tested wheat varieties including Early Premium (EP) under salt stresses. To detect QTL underlying this tolerance, wheat cross EP × HMM was chosen to serve as mapping population that was homozygous at Ppd (photoperiod response gene), Rht (reduced plant height gene) and Vrn (vernalization gene); thus, interference with QTL detection by these loci could be minimized. QTL mapping was conducted firstly using 102 recombinant inbred lines (RILs) that were selected from the EP × HMM population (827 RILs) for similarity in grain yield under non-saline condition. Under salt stresses, however, the 102 RILs varied significantly in grain yield. These RILs were genotyped using a 90 K SNP (single nucleotide polymorphism) array; consequently, a QTL (QSt.nftec-2BL) was detected on chromosome 2B. Then, using 827 RILs and new simple sequence repeat (SSR) markers developed according to the reference sequence IWGSC RefSeq v1.0, location of QSt.nftec-2BL was refined to a 0.7 cM (6.9 Mb) interval flanked by SSR markers 2B-557.23 and 2B-564.09. Selection for QSt.nftec-2BL was performed based on the flanking markers using two bi-parental wheat populations. Trials for validating effectiveness of the selection were conducted in salinized fields in two geographical areas and two crop seasons, demonstrating that wheat plants with the salt-tolerant allele in homozygous status at QSt.nftec-2BL produced higher grain yields by up to 21.4% than otherwise.

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Data availability

All data and materials described in this paper are available from the corresponding author upon request.

Code availability

Not applicable.

Abbreviations

2BL:

The long arm of chromosome 2B

EP:

Early Premium, a common wheat variety

HMM:

Hongmangmai, a common wheat landrace

IWGSC:

International Wheat Genome Sequencing Consortium

LOD:

Logarithm of the odds

PVE:

Phenotypic variance explained

QTL:

Quantitative trait locus/loci

RIL:

Recombinant inbred line

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeat

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Acknowledgements

We thank Lele Luo, Dawei Wang, Xinde Lin, Qiang Ding and Jian He for providing excellent technical assistance in wheat grain yield trials. This study was supported by National Key Research and Development Program of China (2020YFD0900102), Beijing Academy of Agriculture and Forestry Sciences Sci-Tech Innovation Capacity Building Program (KJCX20200115) and National Natural Science Foundation of China (31871923; 32101702).

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Author notes

  1. Xiang Zhang and Xu Jiang contributed equally to this study.

Authors and Affiliations

  1. National Fisheries Technology Extension Center, Beijing, 100125, People’s Republic of China

    Xiang Zhang

  2. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Xu Jiang

  3. Liaoning Provincial Key Laboratory of Forest Protection, Liaoning Academy of Forestry Science, Shenyang, 110032, People’s Republic of China

    Xu Jiang

  4. Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, People’s Republic of China

    Yibin Zhang

  5. Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Junda Ren

  6. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Jing Feng

  7. Institute of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Wei Quan

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  1. Xiang Zhang
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Contributions

XZ and WQ conceived the study and designed and managed the experiments. JF, WQ and XZ developed the wheat populations. XZ, XJ, WQ, JF, YZ and JR performed the phenotyping and/or SSR genotyping. XZ and XJ developed the SSR markers, constructed the genetic maps and statistically analyzed the experimental data. XZ drafted the manuscript. All authors reviewed the manuscript and provided suggestions.

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Correspondence to Jing Feng or Wei Quan.

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Zhang, X., Jiang, X., Zhang, Y. et al. Identification of QTL for reducing loss of grain yield under salt stress conditions in bi-parental populations derived from wheat landrace Hongmangmai. Theor Appl Genet 136, 49 (2023). https://doi.org/10.1007/s00122-023-04290-5

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