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A dominant gene Ihrl1 is tightly linked to and inhibits the gene Ndhrl1 mediating nitrogen-dependent hypersensitive reaction-like phenotype in wheat

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Abstract

Key message

Identification and mapping of an inhibitor of Ndhrl1 mediating nitrogen-dependent hypersensitive reaction-like phenotype in wheat.

Abstract

Hypersensitive reaction-like (HRL) traits are characteristic of spontaneous lesions including yellowish spots, brown spots or white-stripe that appeared randomly and dispersedly on all the leaves in the absence of plant pathogens. Our previous studies have shown that the wheat line P7001 showed an HRL trait at low nitrogen supply, and this trait was controlled by the gene Ndhrl1 (Nitrogen-dependent hypersensitive reaction-like 1). In order to investigate the robustness of the trait expression mediated by Ndhrl1 under different genetic backgrounds, seven genetic populations, with P7001 being the common female parent, were constructed and analyzed. F1 plants from six of the seven combinations showed HRL trait and Ndhrl1 segregated in a dominant way of HRL: non-HRL = 3:1 in the six populations (F2). Exceptionally, the F1 plants of P7001/Fielder combination showed non-HRL trait and HRL trait in the F2 population showed a contrasting recessive segregation ratio of HRL: non-HRL = 1:3, suggesting Fielder may have another HRL-related gene. Using 55 K SNP array and PCR-based markers, the HRL-related gene in Fielder was mapped to an interval of 5.63–12.91 Mb on the short arm of chromosome 2B with the flanking markers Yzu660R075552 and Yzu660F075941. A recombinant with genomic region of Fielder at Ndhrl1 locus showing HRL trait demonstrated that Fielder also harbored Ndhrl1 same as P7001. Thus, Fielder carries a single dominant suppressor of Ndhrl1, designated as Ihrl1 (Inhibitor of hypersensitive reaction-like). Interestingly, Ihrl1 is tightly linked to Ndhrl1 and may be also involved in nitrogen metabolic and (or) signaling pathways.

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Data availability

The data sets generated and analyzed in this study are available upon reasonable request from the corresponding authors.

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Funding

This work was supported by the National Natural Science Foundation of China (31600997, 31271704) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Any opinions, or conclusions expressed in this publication are those of the authors and do not necessarily reflect the view of the funders.

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  1. Lei Li and Jiaqi Liu have equally contributed to this work.

Authors and Affiliations

  1. Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou, China

    Lei Li, Jiaqi Liu, Hao Gong, Yang Zhao, Jinbiao Luo, Zhengxi Sun & Tao Li

  2. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China

    Tao Li

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  1. Lei Li
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Contributions

LL designed the experiments, supervised all analyses and drafted the manuscript; JL co-drafted the manuscript and carried out the primer design and genotyping. HG carried out the mapping of Ndhrl1; YZ and JL carried out the mapping of Ihrl1; ZS and TL conceived the idea of the study and finalized the manuscript. All the authors read and approved the manuscript.

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Correspondence to Tao Li.

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Communicated by Aimin Zhang.

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122_2022_4200_MOESM1_ESM.pdf

Supplementary Figure S1. Phenotype of eight wheat varieties and seven F1 plants. Supplementary Figure S2. The genetic map of P7001/Fielder. Supplementary Table S1. Primer sequence of Indel markers (PDF 832 KB)

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Li, L., Liu, J., Gong, H. et al. A dominant gene Ihrl1 is tightly linked to and inhibits the gene Ndhrl1 mediating nitrogen-dependent hypersensitive reaction-like phenotype in wheat. Theor Appl Genet 135, 3563–3570 (2022). https://doi.org/10.1007/s00122-022-04200-1

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