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A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat

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Abstract

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Using bulk segregant analysis (BSA) coupling with RNA-seq and DNA markers identified a potentially novel nitrogen-dependent lesion mimic gene Ndhrl1 on 2BS in wheat.

Abstract

Lesion mimic (LM) refers to hypersensitive reaction-like (HRL) traits that appear on leaf tissue in the absence of plant pathogens. In a wheat line P7001, LM showed up on the leaves under the 0 g nitrogen (N) treatment, but disappeared when sufficient N was supplied, suggesting that LM is N-responsive and N dosage dependent. Using BSA strategy together with RNA-seq and DNA markers, we identified an N-dependent LM gene (Ndhrl1) and mapped it to the short arm of chromosome 2B using an F5 recombinant inbred population developed from the cross of P7001 × P216. The putative gene was delimited into an interval of 8.1 cM flanked by the CAPS/dCAPS markers 7hrC9 and 7hr2dc14, and co-segregated with the dCAPS marker 7hrdc2. This gene is most likely a novel gene for LM in wheat based on its chromosomal location. Further analysis of RNA-seq data showed that plant–pathogen interaction, nitrogen metabolism, zeatin biosynthesis and plant hormone signal transduction pathways were significantly differentially expressed between LM and non-LM lines.

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Acknowledgments

We thank the financial support provided by the National Natural Science Foundation of China (31270704), the Vital Project of Natural Science of Universities in Jiangsu Province (12KJA210002), the China Postdoctoral Science Foundation (2014M560449), and by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Our thanks also go to Dr. Mei Han and Dr. Tao Su for improving English language of this manuscript.

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    Authors and Affiliations

    1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops; Key Laboratory of Plant Functional Genomics of Ministry of Education; Wheat Research Center, Yangzhou University, Yangzhou, 225009, Jiangsu, China

      Lei Li, Xuan Shi, Fei Zheng, Changcheng Li, Di Wu & Tao Li

    2. College of Plant Protection, Yangzhou University, Yangzhou, 225009, China

      Lei Li & Jincai Wu

    3. Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225000, China

      Guihua Bai

    4. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

      Guihua Bai

    5. USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

      Derong Gao

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    1. Lei Li
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    Communicated by E. Lagudah.

    Xuan Shi and Fei Zheng contributed equally to this work.

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    Li, L., Shi, X., Zheng, F. et al. A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat. Theor Appl Genet 129, 2075–2084 (2016). https://doi.org/10.1007/s00122-016-2758-3

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