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Characterization of Pm65, a new powdery mildew resistance gene on chromosome 2AL of a facultative wheat cultivar

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Abstract

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A new powdery mildew resistance gene that can be readily used in wheat breeding, Pm65, was identified in the facultative wheat cultivar Xinmai 208 and mapped to the terminal region of chromosome 2AL.

Abstract

Wheat powdery mildew, a widely occurring disease caused by the biotrophic fungus Blumeriagraminis f. sp. tritici (Bgt), poses a serious threat to wheat production. A high breeding priority is to identify powdery mildew resistance genes that can be readily used either alone or in gene complexes involving other disease resistance genes. An F2 population and 227 F2:3 families derived from the cross Xinmai 208 × Stardust were generated to map a powdery mildew resistance gene in Xinmai 208, a high-yielding Chinese wheat cultivar. Genetic analysis indicated that Xinmai 208 carries a single dominant powdery mildew resistance gene, designated herein Pm65, and linkage analysis delimited Pm65 to a 0.5 cM interval covering 531.8 Kb (763,289,667–763,821,463 bp) on chromosome 2AL in the Chinese Spring reference sequence. An allelism test indicated that Pm65 is a new gene about 10.3 cM distal to the Pm4 locus. Pm65 was 0.3 cM proximal to Xstars355 and 0.2 cM distal to Xstars356. It conferred near-immunity to 19 of 20 Bgt isolates collected from different wheat-growing regions of the USA. Coming from a high-yield potential cultivar, Pm65 can be directly used to enhance powdery mildew resistance in wheat. The newly developed SSR markers Xstars355 and Xstars356 have the potential to tag Pm65 for wheat improvement.

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Abbreviations

Bgt :

Blumeria graminis f. sp. tritici

cM:

Centimorgan

STS:

Sequence tag site

SSR:

Simple sequence repeat

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Acknowledgements

We are very grateful to Dr. Robert McIntosh of Sydney University for his insightful comments and suggestions. We also thank M. Hargrove and R. Whetten for excellent technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Wheat, Peanut, and Other Field Crops Research Unit, USDA-ARS, Stillwater, OK, 74075, USA

    Genqiao Li & Xiangyang Xu

  2. Plant Science Research Unit, USDA-ARS, Raleigh, NC, 27695, USA

    Christina Cowger

  3. Genetics Department, The University of Georgia, Athens, GA, 30602, USA

    Xuewen Wang

  4. Plant and Soil Science Department, Oklahoma State University, Stillwater, OK, 74078, USA

    Genqiao Li & Brett F. Carver

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  1. Genqiao Li
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  2. Christina Cowger
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  3. Xuewen Wang
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  4. Brett F. Carver
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  5. Xiangyang Xu
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Correspondence to Xiangyang Xu.

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Li, G., Cowger, C., Wang, X. et al. Characterization of Pm65, a new powdery mildew resistance gene on chromosome 2AL of a facultative wheat cultivar. Theor Appl Genet 132, 2625–2632 (2019). https://doi.org/10.1007/s00122-019-03377-2

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