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Fine mapping and molecular characterization of the virescent gene vsp in Upland cotton (Gossypium hirsutum)

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The vsp gene was fine mapped to a 353.7-kb region, and a 201-bp deletion that affected chloroplast development and chlorophyll biosynthesis was found in the candidate gene GhPUR4.

Abstract

Virescent mutations can be used as marker traits in heterosis breeding and can also be used to research chloroplast development, chlorophyll biosynthesis and photosynthesis mechanisms. Here, we obtained a light-sensitive virescent mutant, vsp, that has reduced chlorophyll (Chl) content and abnormal chloroplast development. Then, the virescent space (vsp) gene in the vsp mutant was preliminarily mapped to a 38.32-Mb region of chromosome D04 using a high-density SNP genetic map with a total length of 5384.33 cM and 4472 bin markers. Furthermore, the vsp gene was narrowed down to a 353.7-kb region that contains 15 candidate genes using 484 virescent individuals from an F2 population. Sequence analysis of genes in this region showed that a 201-bp deletion was present in the Gh_D04G1108 (GhPUR4) gene in the vsp mutant. The 201-bp deletion of Gh_D04G1108 caused the deletion of 67 AAs in the GhPUR4 protein. Virus-induced gene silencing (VIGS) of GhPUR4 in normal plants caused reduced GhPUR4 gene expression levels, reduced Chl content, abnormal chloroplast development and virescent true leaves. This study could help us unravel the function of GhPUR4 in chloroplast development and Chl biosynthesis at the early developmental stages of the true leaves in cotton, which could promote the research and application of virescent mutations in cotton heterosis breeding.

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Acknowledgements

This research was funded by the National Key Research and Development Programme of China (Grant number 2016YFD0101400).

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Authors and Affiliations

  1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China

    Guangzhi Mao, Hengling Wei, Wei Hu, Qiang Ma, Meng Zhang, Hantao Wang & Shuxun Yu

  2. College of Life Sciences, Xinyang Normal University, Xinyang, 464000, Henan, China

    Guangzhi Mao

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  1. Guangzhi Mao
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  2. Hengling Wei
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Mao, G., Wei, H., Hu, W. et al. Fine mapping and molecular characterization of the virescent gene vsp in Upland cotton (Gossypium hirsutum). Theor Appl Genet 132, 2069–2086 (2019). https://doi.org/10.1007/s00122-019-03338-9

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