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Development and characterization of a complete set of Triticum aestivum–Roegneria ciliaris disomic addition lines

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A complete set wheat-R. ciliaris disomic addition lines (DALs) were characterized and the homoeologous groups and genome affinities of R. ciliaris chromosomes were determined.

Abstract

Wild relatives are rich gene resources for cultivated wheat. The development of alien addition chromosome lines not only greatly broadens the genetic diversity, but also provides genetic stocks for comparative genomics studies. Roegneria ciliaris (genome ScScYcYc), a tetraploid wild relative of wheat, is tolerant or resistant to many abiotic and biotic stresses. To develop a complete set of wheat-R. ciliaris disomic addition lines (DALs), we undertook a euplasmic backcrossing program to overcome allocytoplasmic effects and preferential chromosome transmission. To improve the efficiency of identifying chromosomes from Sc and Yc, we established techniques including sequential genomic in situ hybridization/fluorescence in situ hybridization (FISH) and molecular marker analysis. Fourteen DALs of wheat, each containing one pair of R. ciliaris chromosomes pairs, were characterized by FISH using four repetitive sequences [pTa794, pTa71, RcAfa and (GAA)10] as probes. One hundred and sixty-two R. ciliaris-specific markers were developed. FISH and marker analysis enabled us to assign the homoeologous groups and genome affinities of R. ciliaris chromosomes. FHB resistance evaluation in successive five growth seasons showed that the amphiploid, DA2Yc, DA5Yc and DA6Sc had improved FHB resistance, indicating their potential value in wheat improvement. The 14 DALs are likely new gene resources and will be phenotyped for more agronomic performances traits.

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Acknowledgements

This research was supported by the National Key Research and Development Program (2016YFD0102001, 2017YFD0100801), International Cooperation and Exchange of the National Natural Science Foundation of China (Grant No. 31661143005), ‘948’ Project of Ministry of Agriculture (2016-X10), Science and Technology Service of CAS (KFJ-STS-ZDTP-002), Shanghai Agriculture Applied Technology Development Program, China (20160101), Jiangsu Province scientific and technological achievements into a special fund project (BA2017138), Indigenous Innovation Foundation of Jiangsu Provincial Agricultural Science and Technology [CX(15)1001], the Program of Introducing Talents of Discipline to Universities (No. B08025), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Lingna Kong, Xinying Song contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, Jiangsu, China

    Lingna Kong, Xinying Song, Jin Xiao, Haojie Sun, Keli Dai, Chunxia Yuan, Shouzhong Zhang, Haiyan Wang & Xiue Wang

  2. Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, D.F., Mexico

    Caixia Lan, Pawan Singh, Ravi Singh, Haiyan Wang & Xiue Wang

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  1. Lingna Kong
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Correspondence to Haiyan Wang or Xiue Wang.

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Communicated by Beat Keller.

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Kong, L., Song, X., Xiao, J. et al. Development and characterization of a complete set of Triticum aestivum–Roegneria ciliaris disomic addition lines. Theor Appl Genet 131, 1793–1806 (2018). https://doi.org/10.1007/s00122-018-3114-6

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