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Genetic analysis of leaf rust resistance genes and associated markers in the durable resistant wheat cultivar Sinvalocho MA

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Abstract

In the cross of the durable leaf rust resistant wheat Sinvalocho MA and the susceptible line Gama6, four specific genes were identified: the seedling resistance gene Lr3, the adult plant resistance (APR) genes LrSV1 and LrSV2 coming from Sinvalocho MA, and the seedling resistance gene LrG6 coming from Gama6. Lr3 was previously mapped on 6BL in the same cross. LrSV1 was mapped on chromosome 2DS where resistance genes Lr22a and Lr22b have been reported. Results from rust reaction have shown that LrSV1 from Sinvalocho is not the same allele as Lr22b and an allelism test with Lr22a showed that they could be alleles or closely linked genes. LrSV1 was mapped in an 8.5-cM interval delimited by markers gwm296 distal and gwm261 proximal. Adult gene LrSV2 was mapped on chromosome 3BS, cosegregating with gwm533 in a 7.2-cM interval encompassed by markers gwm389 and gwm493, where other disease resistance genes are located, such as seedling gene Lr27 for leaf rust, Sr2 for stem rust, QTL Qfhs.ndsu-3BS for resistance to Fusarium gramineum and wheat powdery mildew resistance. The gene LrG6 was mapped on chromosome 2BL, with the closest marker gwm382 at 0.6 cM. Lines carrying LrSV1, LrSV2 and LrG6 tested under field natural infection conditions, showed low disease infection type and severity, suggesting that this kind of resistance can be explained by additive effects of APR and seedling resistance genes. The identification of new sources of resistance from South American land races and old varieties, supported by modern DNA technology, contributes to sustainability of agriculture through plant breeding.

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Acknowledgments

Chinese Spring nulli-tetrasomic and ditelosomic lines, tetraploid wheat (Triticum durum) cultivar Buck Platino and diploid wheat (Triticum monoccoccum) DV92 were kindly provided by Dr. G. Tranquilli from Instituto de Recursos Biológicos—INTA (Argentina). L. Ingala thank a doctoral fellowship from the Argentinean Scientific and Technological Research Council (CONICET) and M. López and M.F. Pergolesi from the Argentinean Agency for Science and Technology Promotion (ANPCyT). The authors wish to thank grants PICT 2003-14437, PICT 2005-38150 and PAE 2007-37108-PID 121 from the Argentinean Agency for Science and Technology Promotion (ANPCyT) and grants Nº 522305 and 522312 from the Argentinean Institute of Agricultural Technology (INTA).

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  1. Instituto de Genética “Ewald A. Favret” CICVyA-INTA CC25 (1712) Castelar, Buenos Aires, Argentina

    L. Ingala, M. López, M. Darino, M. F. Pergolesi, M. J. Diéguez & F. Sacco

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  1. L. Ingala
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  2. M. López
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  3. M. Darino
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  4. M. F. Pergolesi
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  5. M. J. Diéguez
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Correspondence to F. Sacco.

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Communicated by M. Sorrells.

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Ingala, L., López, M., Darino, M. et al. Genetic analysis of leaf rust resistance genes and associated markers in the durable resistant wheat cultivar Sinvalocho MA. Theor Appl Genet 124, 1305–1314 (2012). https://doi.org/10.1007/s00122-012-1788-8

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