Abstract
A dominant male sterility (DGMS) line 79-399-3, developed from a spontaneous mutation in Brassica oleracea var. capitata, has been widely used in production of hybrid cultivars in China. In this line, male sterility is controlled by a dominant gene Ms-cd1. In the present study, fine mapping of Ms-cd1 was conducted by screening a segregating population Ms79-07 with 2,028 individuals developed by four times backcrossing using a male sterile Brassica oleracea var. italica line harboring Ms-cd1 as donor and Brassica oleracea var. alboglabra as the recipient. Bulked segregation analysis (BSA) was performed for the BC4 population Ms79-07 using 26,417 SRAP primer SRAPs and 1,300 SSRs regarding of male sterility and fertility. A high-resolution map surrounding Ms-cd1 was constructed with 14 SRAPs and one SSR. The SSR marker 8C0909 was closely linked to the MS-cd1 gene with a distance of 2.06 cM. Fourteen SRAPs closely linked to the target gene were identified; the closest ones on each side were 0.18 cM and 2.16 cM from Ms-cd1. Three of these SRAPs were successfully converted to dominant SCAR markers with a distance to the Ms-cd1 gene of 0.18, 0.39 and 4.23 cM, respectively. BLAST analysis with these SCAR marker sequences identified a collinear genomic region about 600 kb in scaffold 000010 on chromosomeA10 in B. rapa and on chromosome 5 in A. thaliana. These results provide additional information for map-based cloning of the Ms-cd1 gene and will be helpful for marker-assisted selection (MAS).
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Acknowledgments
The authors thank Dr. Mu Zhuang (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) for kindly providing B. oleacea SSR markers. This research was financed by funds from National 863 High Technology Programme, P.R. China (2006AA100108) and National Science Foundation of China (30972010). The work was done in the Key Lab of Vegetable Genetics and Physiology of the Ministry of Agriculture, P.R. China and Sino-Dutch Joint Lab of Horticultural Genomics Technology.
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Communicated by H. Becker.
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Zhang, X., Wu, J., Zhang, H. et al. Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea . Theor Appl Genet 123, 231–238 (2011). https://doi.org/10.1007/s00122-011-1579-7
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DOI: https://doi.org/10.1007/s00122-011-1579-7