Skip to main content
SpringerLink
Log in

Identification of quantitative trait loci for resistance to Xanthomonas campestris pv. campestris in Brassica rapa

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Resistance to six known races of black rot in crucifers caused by Xanthomonas campestris pv. campestris (Pammel) Dowson is absent or very rare in Brassica oleracea (C genome). However, race specific and broad-spectrum resistance (to type strains of all six races) does appear to occur frequently in other brassica genomes including B. rapa (A genome). Here, we report the genetics of broad spectrum resistance in the B. rapa Chinese cabbage accession B162, using QTL analysis of resistance to races 1 and 4 of the pathogen. A B. rapa linkage map comprising ten linkage groups (A01–A10) with a total map distance of 664 cM was produced, based on 223 AFLP bands and 23 microsatellites from a F2 population of 114 plants derived from a cross between the B. rapa susceptible inbred line R-o-18 and B162. Interaction phenotypes of 125 F2 plants were assessed using two criteria: the percentage of inoculation sites in which symptoms developed, and the severity of symptoms per plant. Resistance to both races was correlated and a cluster of highly significant QTL that explained 24–64% of the phenotypic variance was located on A06. Two additional QTLs for resistance to race 4 were found on A02 and A09. Markers closely linked to these QTL could assist in the transference of the resistance into different B. rapa cultivars or into B. oleracea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bain DC (1952) Reaction of Brassica seedlings to black rot. Phytopathology 42:497–500

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Chyi YS, Hoenecke ME, Sernyk JL (1992) A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn campestris). Genome 35:746–757

    CAS  Google Scholar 

  • Guo H, Dickson MH, Hunter JE (1991) Brassica napus sources of resistance to black rot in crucifers and inheritance of resistance. HortScience 22:108–109

    Google Scholar 

  • Hansen LN, Earle ED (1995) Transfer of resistance to Xanthomonas campestris pv campestris into Brassica oleracea L. by protoplast fusion. Theor Appl Genet 91:1293–1300

    Article  CAS  Google Scholar 

  • Hee Kim J, Chang-Soon J, Kyoo-Won C, Yong Pyo L (1999) AFLP and RAPD mapping of Chinese cabbage (Brassica rapa L. var. pekinensis). International Plant & Animal Genome VII Conference, San Diego, p141

  • Ignatov AN, Kuginuki Y, Suprunova TP, Pozmogova GE, Seitova AM, Dorokhov DB, Hirai M (2000) RAPD markers linked to locus controlling resistance for race 4 of the black rot causative agent, Xanthomonas campestris pv. campestris (Pamm.) Dow. in Brassica rapa L. Russ J Genet 36:281–283

    CAS  Google Scholar 

  • Jansen RC (1993) Interval mapping of multiple quantitative trait loci. Genetics 135:205–211

    PubMed  CAS  Google Scholar 

  • Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455

    PubMed  CAS  Google Scholar 

  • Kole C, Williams PH, Rimmer SR, Osborn TC (2002) Linkage mapping of genes controlling resistance to white rust (Albugo candida) in Brassica rapa (syn. campestris) and comparative mapping to Brassica napus and Arabidopsis thaliana. Genome 45:22–27

    Article  PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenics 12:172–175

    Google Scholar 

  • Lander ES, Kruglyak L (1995) Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nat Genet 11:241–247

    Article  PubMed  CAS  Google Scholar 

  • Lowe AJ, Moule C, Trick M, Edwards KJ (2004) Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theor Appl Genet 108:1103–1112

    Article  PubMed  CAS  Google Scholar 

  • Manzanares-Daulex MJ, Delourne R, Baron F, Thomas G (2000) Mapping of one major gene and of QTL involved in resistance to clubroot in Brassica napus. Theor Appl Genet 101:885–891

    Article  Google Scholar 

  • Matsumoto E, Yasui C, Ohi M, Tsukada M (1998) Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis). Euphytica 104:79–86

    Article  CAS  Google Scholar 

  • Nováková B, Salava J, Lydiate D (1996) Construction of a genetic linkage map for Brassica campestris L. (syn. Brassica rapa L.). Genetika a Slechteni 32:249–256

    Google Scholar 

  • Pammel LH (1895) Bacteriosis of rutabaga (Bacillus campestris n sp.). Iowa State Coll Agric Exp Stn Bull 27:130–134

    Google Scholar 

  • Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of amphiploid Brassica napus (oilseed rape). Genome 38:1122–1133

    CAS  PubMed  Google Scholar 

  • Pilet ML, Delourne R, Foisset N, Renard M (1998) Identification of loci contributing to quantitative field resistance to blackleg disease, causal agent Leptosphaeria maculans (Desm.) Ces. et de Not., in winter rapeseed (Brassica napus L.). Theor Appl Genet 96:23–30

    Article  Google Scholar 

  • SAS (2000) The SAS System. SAS Online Doc. HTML Format Version eight. SAS Institute Inc., Cary, North Carolina, USA

  • Schaad NW, Thaveeschai N (1983) Black rot of crucifers in Thailand. Plant Dis 67:1231–1234

    Google Scholar 

  • Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa (syn. campestris) based on restriction fragments length polymorphism loci. Theor Appl Genet 82:296–304

    Article  CAS  Google Scholar 

  • Suwabe K, Iketani H, Nunome T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098

    Article  PubMed  CAS  Google Scholar 

  • Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Nunome T, Fukuoka H, Hirai M, Matsumoto S (2004) A microsatellite-based linkage map of Brassica rapa L.: synteny analysis with Arabidopsis and QTL analysis for clubroot resistance. International Plant & Animal genome XII Conference, San Diego, USA

  • Taylor JD, Conway J, Roberts SJ, Astley D, Vicente JG (2002) Sources and origin of resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92:105–111

    CAS  PubMed  Google Scholar 

  • Teutónico RA, Osborn TC (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of Brassica napus, Brassica oleracea, and Arabidopsis thaliana. Theor Appl Genet 89:885–894

    Article  Google Scholar 

  • Tonguç M, Griffiths PD (2004) Development of black rot resistant interspecific hybrids between Brassica oleracea L. cultivars and Brassica accession A 19482, using embryo rescue. Euphytica 136:313–318

    Article  Google Scholar 

  • Van Ooijen JW (1999) LOD significance threshold for QTL analysis in experimental populations of diploid species. Heredity 83:613–624

    Article  PubMed  Google Scholar 

  • Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C (2002) MapQTL® 4.0, Software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen, the Netherlands

  • Van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands

  • Vicente JG (2004) A podridão negra das crucíferas.In: Lopes G (eds) COTHN Centro Operativo e Tecnologico Hortofruticola Nacional. Alcobaça, Portugal, pp 102

    Google Scholar 

  • Vicente JG, Conway J, Roberts SJ, Taylor JD (2001) Identification and origin of Xanthomonas campestris pv. campestris races and related pathovars. Phytopathology 91:492–499

    CAS  PubMed  Google Scholar 

  • Vicente JG, Taylor JD, Sharpe AG, Parkin IAP, Lydiate DJ, King GJ (2002) Inheritance of race-specific resistance to Xanthomonas campestris pv. campestris in Brassica genomes. Phytopathology 92:1134–1141

    CAS  PubMed  Google Scholar 

  • Voorrips RE (2002) MapChart, software for the graphical presentation of linkage maps and QTLs. J Heredity 93:77–78

    Article  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, ReijansM Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Vuylsteke M, Mank R, Antonise R, Bastiaans E, Senior ML, Stuber CW, Melchinger AE, Lübberstedt T, Xia XC, Stam P, Zabeau M, Kuiper M (1999) Two high-density AFLP® linkage maps of Zea mays L.: analysis of distribution of AFLP markers. Theor Appl Genet 99:921–935

    Article  CAS  Google Scholar 

  • Waugh P, Bonar N, Baird E, Thomas B, Craner A, Hayes P, Powell W (1997) Homology of AFLP products in three mapping populations of barley. Mol Gen Genet 255:312–321

    Google Scholar 

  • Westman AL, Kresovich S, Dickson MH (1999) Regional variation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassisicola and Xanthomonas campestris pv. campestris. Euphytica 106:253–259

    Article  Google Scholar 

  • Williams PH (1980) Black rot: a continuing threat to world crucifers. Plant Dis 64:736–742

    Article  Google Scholar 

  • Zhao J, Meng J (2003) Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotium in oilseed Brassica napus. Theor Appl Genet 106:759–764

    PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the UK Department for Environment, Food and Rural Affairs (Defra). P. Soengas was supported by a grant from the British Council/Fundación Barrié de la Maza and a grant from the Xunta de Galicia (Spain). The authors also thank Prof. E. Holub for critical reading of the manuscript, Prof. Yong Pyo Lim for access to B. rapa map data and Dr. Keita Suwabe for sharing SSR data in advance of publication.

Author information

Authors and Affiliations

  1. Warwick HRI, The University of Warwick, Wellesbourne, CV35 9EF, UK

    P. Soengas, P. Hand, J. G. Vicente, J. M. Pole & D. A. C. Pink

  2. Misión Biológica de Galicia (CSIC), Aptdo. 28, 36080, Pontevedra, Spain

    P. Soengas

Authors
  1. P. Soengas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Hand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. G. Vicente
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. M. Pole
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. A. C. Pink
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Soengas.

Additional information

Communicated by H. C. Becker.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soengas, P., Hand, P., Vicente, J.G. et al. Identification of quantitative trait loci for resistance to Xanthomonas campestris pv. campestris in Brassica rapa . Theor Appl Genet 114, 637–645 (2007). https://doi.org/10.1007/s00122-006-0464-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-006-0464-2

Keywords

Search

Navigation