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Genetic evidence for gametophytic selection of wilt resistant alleles in chickpea

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Abstract

Gametophytic selection can drastically reduce the number of selection cycles during crop improvement programs. The objective of the present investigation was to test whether the nature of inheritance of two unlinked disease-resistant loci, h 1 and h 2, against Fusarium wilt in chickpea (Cicer arietinum L.) under gametophytic (pollen) selection was similar to that already observed at sporophytic level. A homozygous dominant (H 1 H 1 H 2 H 2) susceptible genotype JG-62 was crossed to a recessive (h 1 h 1 h 2 h 2) resistant genotype WR-315 to produce 20 F1 hybrid seeds. In the following generation, flower buds of 10 F1 hybrid plants were subjected to toxin stress before anthesis and the remaining ten control F1 plants’ flowers were sprayed with water. Thirty-four selected BC1 plants were generated by test crossing resistant WR-315 individuals with pollen from toxin-stressed F1 individuals. Both control and treated F1 plants were selfed to produce respective F2 generations. Two DNA markers, CS-27700bp and A07C430bp, linked to susceptible alleles H 1 and H 2, respectively, were used to study the inheritance patterns of h 1 and h 2 loci in the F2 and BC1 generations. One hundred and forty-four selected F2, 129 control F2, and 34 selected backcross individuals were tested for the presence or absence of DNA markers. Except for the control F2, observed ratios of selected F2 and BC1 populations exhibited significant chi-square deviations from expected monogenic and digenic ratios. Our results suggest that gametophytic selection is as effective as that realized at the sporophytic level, and that the gametophytic selection can be an effective breeding tool for plant breeding programs.

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Acknowledgments

We thank two anonymous reviewers for their useful comments on an earlier version of the manuscript, and R. L. Ravikumar thanks the Defense Research and Development Organisation (DRDO), Government of India, for their financial support to carry out the research.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, 580005, India

    R. L. Ravikumar, B. S. Patil & C. D. Soregaon

  2. Department of Botany and Plant Sciences, University of California, Riverside, CA, 92521-0125, USA

    S. G. Hegde

Authors
  1. R. L. Ravikumar
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  2. B. S. Patil
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  3. C. D. Soregaon
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  4. S. G. Hegde
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Correspondence to R. L. Ravikumar.

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Communicated by M. Kearsey.

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Ravikumar, R.L., Patil, B.S., Soregaon, C.D. et al. Genetic evidence for gametophytic selection of wilt resistant alleles in chickpea. Theor Appl Genet 114, 619–625 (2007). https://doi.org/10.1007/s00122-006-0462-4

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  • DOI: https://doi.org/10.1007/s00122-006-0462-4

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