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New sources of downy mildew resistance in cauliflower and their prospects in resistance breeding

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Abstract

204 genotypes of cauliflower were screened against downy mildew (DM) through challenge inoculation during 2019–20 and 2020–21 and 12 genotypes were identified as resistant (DS < 1). The disease reaction of the genotypes was recorded under natural infection conditions at curding and flowering stages. The DM score at the flowering stage showed a strong positive correlation with challenge inoculation (r2 = 0.614). The inheritance of downy mildew resistance was determined using 10 F2s populations developed from three susceptible(S) varieties (Pusa Himjyoti, Pusa Sharad, Pusa Meghna) and five resistant (R) genotypes (BR-2, 3–5–1-1, CCm, BR-207, BR-161). The F2 plants expressed segregation into R and S plants as per the expected Mendelian ratio of 3:1 (p = 0.3–0.7) and revealed the monogenic dominant nature of resistance. The segregation of 118 F2:3 progenies and 121 RILs from Pusa Himjyoti × BR-2 and two confirmatory F2s from S × S (Pusa Himjyoti × Pusa Sharad) and R × R (3–5–1-1 × CCm) also confirmed the single gene inheritance. Uniform segregation ratio in diverse resistance sources along with three different susceptible backgrounds established the single gene theory of DM resistance in Indian cauliflower germplasm. These new sources for downy mildew resistance having a simple Mendelian inheritance patterns can be directly used in resistance breeding.

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Acknowledgements

The authors express thanks to Director, IARI, New Delhi for facilities. The part of the study was from the first author’s Ph.D. thesis. Hence, we acknowledge PG School, IARI, New Delhi. We thank ICAR for CRP Hybrid Technology Project [Project Code: IARI/CRPHT-12-142-F (Cauliflower)] for financial support for part of the work. We thank Head, IARI RS, Katrain, Himachal Pradesh, India for sharing 20 lines of late cauliflower.

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  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Shrawan Singh, P. Kalia & S. R. Sharma

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  1. Shrawan Singh
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  2. P. Kalia
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Correspondence to Shrawan Singh.

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Singh, S., Kalia, P. & Sharma, S.R. New sources of downy mildew resistance in cauliflower and their prospects in resistance breeding. Eur J Plant Pathol 163, 813–826 (2022). https://doi.org/10.1007/s10658-022-02517-7

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