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Genetic characterization and molecular mapping of Hessian fly resistance genes derived from Aegilops tauschii in synthetic wheat

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Abstract

Two synthetic hexaploid wheat lines (×Aegilotriticum spp., 2n = 6x = 42, genomes AABBDD), SW8 and SW34, developed from the crosses of the durum wheat cultivar Langdon (Triticum turgidum L. var. durum, 2n = 4x = 28, genomes AABB) with two Aegilops tauschii Cosson accessions (2n = 2x = 14, genome DD), were determined to carry Hessian fly [Mayetiola destructor (Say)] resistance genes derived from the Ae. tauschii parents. SW8 was resistant to the Hessian fly biotype Great Plains (GP) and strain vH13 (virulent to H13). SW34 was resistant to biotype GP, but susceptible to strain vH13. Allelism tests indicated that resistance genes in SW8 and SW34 may be allelic to H26 and H13 or correspond to paralogs at both loci, respectively. H26 and H13 were localized to chromosome 4D and 6D, respectively, in previous studies. Molecular mapping in the present study, however, assigned the H26 locus to chromosome 3D rather than 4D. On the other hand, mapping of the resistance gene in SW34 verified the previous assignment of the H13 locus to chromosome 6D. Linkage analysis and physical mapping positioned the H26 locus to the chromosomal deletion bin 3DL3-0.81–1.00. A linkage map for each of these two resistance genes was constructed using simple sequence repeat (SSR) and target region amplification polymorphism (TRAP) markers.

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References

  • Berzonsky WA, Ding H, Haley SD, Harris MO, Lamb RJ, Mckenzie RIH, Ohm HW, Patterson FL, Peairs F, Porter DR, Ratcliffe RH, Shanower TG (2003) Breeding wheat for resistance to insects. Plant Breed Rev 22:221–296

    Google Scholar 

  • Carlson SK, Patterson FL, Gallun RL (1978) Inheritance of resistance to Hessian fly derived from Triticium turgidum L. Crop Sci 18:1011–1014

    Article  Google Scholar 

  • Cartwright WB, LaHue DW (1944) Testing wheats in the greenhouse for Hessian fly resistance. J Econ Entomol 37:385–387

    Google Scholar 

  • Cox TS, Hatchett JH (1994) Hessian fly resistance gene H26 transferred from Triticum tauschii to common wheat. Crop Sci 34:958–960

    Article  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation. Version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Dweikat I, Zhang W, Ohm H (2002) Development of STS markers linked to Hessian fly resistance gene H6 in wheat. Theor Appl Genet 105:766–770

    Article  PubMed  CAS  Google Scholar 

  • Dweikat I, Ohm H, Patterson F, Cambron S (1997) Identification of RAPD markers for 11 Hessian fly resistance genes in wheat. Theor Appl Genet 94:419–423

    Article  CAS  Google Scholar 

  • Dweikat I, Ohm H, Mackenzie S, Patterson F, Cambron S, Ratcliffe R (1994) Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Theor Appl Genet 89:964–968

    Article  CAS  Google Scholar 

  • Friesen TL, Xu S, Faris JD, Rasmussen JB, Miller JD, Harris MO (2003) Evaluation of Langdon durum-Aegilops tauschii synthetic hexaploid wheats for reaction to tan spot, Stagonospora leaf blotch, leaf rust, stem rust, and Hessian fly. pp 1133–1135. In: Pogna NE, Romanò M, Pogna EA, Galterio G (eds) Proceedings of the 10th international wheat genetics symposium, Pasetum, Italy. 1–6 Sept. 2003. Istituto Sperimentale per la Cerealicoltura, Rome, Italy

  • Gallun RL, Patterson FL (1977) Monosomic analysis of wheat for resistance to Hessian fly. J Hered 68:223–236

    Google Scholar 

  • Gill BS, Wilson DL, Raupp WJ, Hatchett JH, Cox TS, Amri S, Sears RG (1991a) Registration of KS89WGRC3 and KS89WGRC6 Hessian fly-resistant hard red winter wheat germplasm. Crop Sci 31:245

    Article  Google Scholar 

  • Gill BS, Wilson DL, Raupp WJ, Hatchett JH, Cox TS, Amri S, Sears RG (1991b) Registration of KS89WGRC4 hard red winter wheat germplasm with resistance to Hessian fly, greenbug, and soil-borne mosaic virus. Crop Sci 31:246

    Google Scholar 

  • Gill BS, Raupp WJ (1987) Direct genetic transfer from Aegilops squarrosa L. to hexaploid wheat. Crop Sci 27:445–450

    Article  Google Scholar 

  • Gill BS, Hatchett HJ, Raupp WJ (1987) Chromosomal mapping of Hessian fly resistant gene H13 in the D genome of wheat. J Hered 78:97–100

    Google Scholar 

  • Gill BS, Hatchett JH, Raupp WJ, Sears RG, Martin TJ (1986) Registration of KS85WGRC01 Hessian fly resistant hard red winter wheat germplasm. Crop Sci 26:1266–1267

    Article  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Roder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Harris, MO, Anderson KG, Anderson KM, Kanno H (2006) Proximate cues for reduced oviposition on wheat plants attacked by conspecific larvae. Environ Entomol (in press)

  • Hatchett JH, Starks KJ, Webster JA (1987) Insect and mite pests of wheat. In: Heyneg EG (eds) Wheat and wheat improvement. 2nd edn. Am Soc Agron, Madison, WI pp 626–675

    Google Scholar 

  • Hatchett JH, Gill BS (1981) D-genome sources of resistance in Triticum tauschii to Hessian fly. J Hered 72:126–127

    Google Scholar 

  • Hatchett JH, Martin TJ, Livers RW (1981) Expression and inheritance of resistance to Hessian fly in synthetic hexaploid wheats derived from Triticum tauschii (Coss) Schmal. Crop Sci 21:731–734

    Article  Google Scholar 

  • Hu J, Vick BA (2006) Integration of the conserved telomere sequence-derived TRAP markers onto an existing sunflower SSR linkage map. Plant Genome XIV. January 14–18, San Diego, CA

  • Hu J, Vick BA (2003) TRAP (target region amplification polymorphism), a novel marker technique for plant genotyping. Plant Mol Biol Rep 21:289–294

    Article  CAS  Google Scholar 

  • Kong L, Ohm HW, Cambron SE, Williams CE (2005) Molecular mapping determines that Hessian fly resistant gene H9 is located on chromosome 1A of wheat. Plant Breed 124:525–531

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander E, Abrahamson J, Barlow A, Daly M, Lincoln S, Newburg L, Green P (1987) Mapmaker: a computer package for constructing genetic-linkage maps. Cytogenet Cell Genet 46:642

    Google Scholar 

  • Liu XM, Brown-Guedira GL, Hatchett JH, Owuoche JO, Chen MS (2005a) Genetic characterization and molecular mapping of a Hessian fly-resistance gene transferred from T. turgidum ssp. dicoccum to common wheat. Theor Appl Genet 111:1308–1315

    Article  CAS  Google Scholar 

  • Liu XM, Fritz AK, Reese JC, Wilde GE, Gill BS, Chen MS (2005b) H9, H10, and H11 compose a cluster of Hessian fly-resistance genes in the distal gene-rich region of wheat chromosome 1AS. Theor Appl Genet 110:143–1480

    Google Scholar 

  • Liu XM, Gill BS, Chen MS (2005c) Hessian fly resistance gene H13 is mapped to a distal cluster of resistance genes in chromosome 6DS of wheat. Theor Appl Genet 111:243–249

    Article  CAS  Google Scholar 

  • Ma ZQ, Gill BS, Sorrells ME, Tanksley SD (1993) RFLP markers linked to Hessian fly-resistance genes in wheat (Triticum aestivum L.) from Triticum tauschii (Coss.) Schmal. Theor Appl Genet 85:750–754

    Article  CAS  Google Scholar 

  • Martin TJ, Harvey TL, Hatchett JH (1982) Registration of greenbug and Hessian fly resistance wheat germplasm. Crop Sci 22:1089

    Article  Google Scholar 

  • McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers J, Appels R (2003) Catalogue of gene symbols for wheat. MacGene 2003 (http://www.grs.nig.ac.jp/wheat/komugi/genes/download.jsp, verified 1 Nov. 2005)

  • Ohm HW, Sharma HC, Patterson FL, Ratcliffe RH, Obanni M (1995) Linkage relationships among genes on wheat chromosome 5A that condition resistance to Hessian fly. Crop Sci 35:1603–1607

    Article  Google Scholar 

  • Pestsova E, Ganal MW, Roder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  PubMed  CAS  Google Scholar 

  • Ratcliffe RH, Cambron SE, Flanders KL, Bosque-Perez NA, Clement SL, Ohm HW (2000) Biotype composition of Hessian fly (Diptera: Cecidomyiidae) populations from the southeastern, mid-western, and northwestern United States and virulence to resistance genes in wheat. J Econ Entomol 94:1319–1328

    Article  Google Scholar 

  • Ratcliffe RH, Safranski GG, Patterson FL, Ohm HW, Taylor PL (1994) Biotype status of Hessian fly (Diptera: Cecidomyiidae) populations from the Eastern United States and their response to 14 Hessian fly resistance genes. J Econ Entomol 87:1113–1121

    Google Scholar 

  • Ratcliffe RH, Hatchett JH (1997) Biology and genetics of the Hessian fly and resistance in wheat. In: Bondari K (eds) New developments in entomology. Research Signpost Publ., Scientific Information Guild, Trivandrum, India pp 47–56

    Google Scholar 

  • Raupp WJ, Amri A, Hatchett JH, Gill BS, Wilson DL, Cox TS (1993) Chromosomal location of Hessian fly resistance genes H22, H23, and H24 derived from Triticum tauschii in the D genome of wheat. J Hered 84:142–145

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Sardesai N, Nemacheck JA, Subramanyam S, Williams CE (2005) Identification and mapping of H32, a new wheat gene conferring resistance to Hessian fly. Theor Appl Genet 111:1167–1173

    Article  PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Cadalen T, Guyomarc’h H, Snape JW, Perretant MR, Charmet G, Boeuf C, Bernard S, Bernard M (2003) An update of the Courtot-Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat. Theor Appl Genet 106:530–538

    PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25

    Article  PubMed  CAS  Google Scholar 

  • Stebbins NB, Patterson FL, Gallun RL (1982) Interrelationships among wheat genes H3, H6, H9, and H10 for Hessian fly resistance. Crop Sci 22:1029–1032

    Article  Google Scholar 

  • Xu SS, Cai X, Wang T, Harris MO, Friesen,TL (2006) Registration of two synthetic hexaploid wheat germplasms resistant to Hessian Fly. Crop Sci (in press)

  • Williams CE, Collier CC, Sardesai N, Ohm HW, Cambron SE (2003) Phenotypic assessment and mapped markers for H31, a new wheat gene conferring resistance to Hessian fly (Diptera: Cecidomyiidae). Theor Appl Genet 107:1516–1523

    Article  PubMed  CAS  Google Scholar 

  • Wilson DL, Gill BS, Raupp WJ, Hatchett JH (1989) Chromosomal mapping of three new Hessian fly resistance genes in common wheat, derived from Aegilops squarrosa. Agron Abstr ASA. Madison, WI p 106

    Google Scholar 

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Acknowledgments

The authors thank Kirk Anderson and Karin Anderson (Department of Entomology, North Dakota State University, Fargo, ND) for their help in maintaining and providing Hessian flies to this research.

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Authors and Affiliations

  1. Department of Plant Science, North Dakota State University, Fargo, ND, 58105, USA

    Tao Wang, Liwang Liu & Xiwen Cai

  2. USDA-ARS, Northern Crop Science Laboratory, P.O. Box 5677, Fargo, ND, 58105, USA

    Steven S. Xu & Jinguo Hu

  3. Department of Entomology, North Dakota State University, Fargo, ND, 58105, USA

    Marion O. Harris

Authors
  1. Tao Wang
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  2. Steven S. Xu
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  3. Marion O. Harris
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  4. Jinguo Hu
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  5. Liwang Liu
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  6. Xiwen Cai
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Correspondence to Xiwen Cai.

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Communicated by F. Ordon

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Wang, T., Xu, S.S., Harris, M.O. et al. Genetic characterization and molecular mapping of Hessian fly resistance genes derived from Aegilops tauschii in synthetic wheat. Theor Appl Genet 113, 611–618 (2006). https://doi.org/10.1007/s00122-006-0325-z

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  • DOI: https://doi.org/10.1007/s00122-006-0325-z

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