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Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines

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Abstract

Quantitative trait loci (QTLs) that control the performance of tissue culture in rice were detected by using 116 RFLP markers and 183 BC1F3 lines derived from two varieties, Koshihikari and Kasalath. With time, the seed callus of Koshihikari tends to turn brown and stop growing, while that of Kasalath remains yellowish-white and proliferates continuously. The performance of tissue culture in the induction of calli from seed, the subculture of induced calli, and shoot regeneration were evaluated by five indices: induced-callus weight, induced-callus color, subcultured-callus volume, subcultured-callus color, and regeneration rate. Through callus induction and subculture, eight putative QTLs (P < 0.001) were located on chromosomes 1, 4, and 9. Among these QTLs, five Kasalath alleles and three Koshihikari alleles improved tissue culture performance. No QTL for regeneration was found. Among all the QTLs, qSv1 explained the largest phenotypic variance, 33%, in subcultured-callus volume. In induced-callus color, two detected QTLs accounted for 36.4% of the total phenotypic variance; this was the highest score among the five indices used to evaluate the performance of tissue culture. Three near-isogenic lines for QTLs, located in two regions on chromosome 1, were developed to evaluate their tissue culture performance. The Kasalath alleles in qSv1 and qSc1-1 improved callus color through callus induction and subculture, and increased the subcultured-callus volume and the fresh weight of regenerated calli, including shoots, roots, and differentiated structures. In qSc1-2, the Kasalath allele improved callus color through induction and subculture. These results verified the presence of QTLs for the volume and color of subcultured callus on chromosome 1, qSv1, qSc1-1, and qSc1-2.

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Abbreviations

ICW:

Induced-callus weight

ICC:

Induced-callus color

SCV:

Subcultured-callus volume

SCC:

Subcultured-callus color

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Acknowledgments

We thank Mr. Liew Yike Hwai (Tsukuba University) for his technical assistance in tissue culture. This work was supported by grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Rice Genome Project) and the Sumitomo Foundation 2003.

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Author notes

  1. T. Yamamoto

    Present address: Honda Research Institute Japan Co., Ltd., Creation Core Kazusa, 2-1-4 Kazusa-Kamatari, 292-0812, Kisarazu, Chiba, Japan

Authors and Affiliations

  1. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, 305-8602, Tsukuba, Ibaraki, Japan

    F. Taguchi-Shiobara & S. Oka

  2. Rice Genome Research Program (RGP), National Institute of Agrobiological Sciences/Institute of Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, 446-1, Ippaizuka, 305-0854, Kamiyokoba, Tsukuba, Ibaraki, Japan

    T. Yamamoto & M. Yano

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  1. F. Taguchi-Shiobara
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  4. S. Oka
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Correspondence to F. Taguchi-Shiobara.

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Communicated by Q. Zhang

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Taguchi-Shiobara, F., Yamamoto, T., Yano, M. et al. Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines. Theor Appl Genet 112, 968–976 (2006). https://doi.org/10.1007/s00122-005-0200-3

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  • DOI: https://doi.org/10.1007/s00122-005-0200-3

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