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Pine heartwood and glass surfaces: easy method to test the fate of bacterial contamination

Kiefernkernholz und Glasoberflächen: eine einfache Methode zur Prüfung von Bakterienbefall

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Abstract

The survival of two bacteria, Escherichia coli and Listeria monocytogenes was observed on pine heartwood and glass surfaces by using a simple test method. The development of the number of bacterial cells was evaluated by titration after vortexing the samples in BHI broth and culturing the resulting broth on agar plates. The bacterial count decreased clearly faster on pine heartwood than on glass surfaces. This result was confirmed by studying the wooden samples also one day after to exclude possible adherence of the bacterial cells on the porous surface. This study confirms the results of several other studies that suggest wood to have antibacterial properties.

Zusammenfassung

Das Überleben von zwei Bakterienarten, Escherichia coli und Listeria monocytogenes, auf Kiefernkernholz und Glasoberflächen wurde mit einer einfachen Prüfmethode untersucht. Mittels Titrierung wurde die Entwicklung der Anzahl der Bakterienzellen untersucht, nachdem die Proben in einer BHI-Nährlösung gevortext wurden, die anschließend auf Agarplatten kultiviert wurde. Die Keimzahl nahm auf Kiefernkernholz deutlich schneller ab als auf Glasoberflächen.

Dieses Ergebnis konnte auch durch Untersuchungen der Holzproben am darauf folgenden Tag bestätigt werden, um ein mögliches Anhaften der Bakterienzellen auf der porösen Oberfläche auszuschließen. Mit dieser Studie konnten die Ergebnisse früherer Untersuchungen bestätigt werden, die Holz antibakterielle Eigenschaften zuschreiben.

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Correspondence to Tiina Vainio-Kaila.

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Vainio-Kaila, T., Kyyhkynen, A., Viitaniemi, P. et al. Pine heartwood and glass surfaces: easy method to test the fate of bacterial contamination. Eur. J. Wood Prod. 69, 391–395 (2011). https://doi.org/10.1007/s00107-010-0453-7

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  • DOI: https://doi.org/10.1007/s00107-010-0453-7

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