Organoids can be established from human dental follicle
To develop epithelial organoids starting from human tooth, the DF, known not only to encompass a large mesenchymal component but also the small epithelial ERM compartment [5], was isolated from unerupted third molars (wisdom teeth) extracted from adolescent patients (Fig. 1a). After tissue dissociation, the epithelial–mesenchymal cell mixture, comprising single cells and cell clusters, was embedded in Matrigel and cultured in a precisely defined medium. Organoids are typically established using a cocktail of growth and regulatory factors active in the tissue’s epithelial stem cell niche. In case niche signals are unresolved, factors with a key role in the tissue’s embryonic development are applied [13,14,15,16,17]. Hence, since the human tooth epithelial stem cell niche is undefined, we tested growth and signaling factors shown to play a role in tooth development, including sonic hedgehog (SHH), fibroblast growth factors (FGFs) and insulin-like growth factor-1 (IGF1) [23, 24]. We started with a medium containing these factors as well as generic organoid growth factors (such as WNT activators, nicotinamide, BMP inhibitor and p38 MAPK inhibitor) [13,14,15,16, 25] and assessed the essentiality of individual factors by omitting. The BMP inhibitor (Noggin), p38 MAPK inhibitor (SB202190), WNT activator (R-spondin 1 (RSPO1)), IGF1, SHH, nicotinamide, and FGFs (FGF2, FGF8, FGF10) were all evidently needed for efficient organoid formation (Supplementary Fig. 1a). Eventually, this systematic evaluation led to defining an optimized medium (further referred to as tooth organoid medium or TOM; Supplementary Table 1), allowing to develop organoid lines from DF samples at 100% efficiency (Supplementary Table 2).
Organoid structures gradually developed in 2 weeks’ time (Fig. 1a; passage 0 (P0)), growing out of cell clusters (typically 4–8 cells wide by 20 cells long, similar to the ERM cell groups present in the primary DF tissue; Supplementary Fig. 1b,c) [5, 26], or of single cells, indicating the capability of clonal development (Supplementary Fig. 1b). Importantly, the organoids were amenable to long-term expansion, at present for more than 10 consecutive passages (i.e. 5 months) (Fig. 1a). At passaging, the organoids were dissociated into single cells and organoid structures efficiently re-grew. When starting from a mixture of cells dissociated from either eGFP+ or eGFP− organoid cultures, the organoids that reformed were homogeneously fluorescent or non-fluorescent, suggesting clonal regrowth at passaging (Supplementary Fig. 1d). Mesenchymal cells, also present in the dissociated DF cell mixture, adhered to the bottom of the culture plate following sample seeding (P0; Supplementary Fig. 1e), and were swiftly lost at passaging in the standard, epithelial-favoring organoid culture conditions used (P1; Supplementary Fig. 1e). During a single-passage 14-day culture period, organoids progressively increased in size while the proportion of proliferating (KI67+) cells gradually decreased and the fraction of apoptotic (cleaved-caspase 3, CC3+) cells slightly enhanced, although to only low levels which remained invariable over different passages (as determined in full-grown day-14 organoids) (Supplementary Fig. 1f,g). Full-grown organoid size also remained constant over passaging, after a first significant increase from P0 to P1 (Supplementary Fig. 1g). Within individual passages, the organoids displayed considerable size homogeneity (Supplementary Fig. 1g). Finally, organoid cultures could be reconstituted after cryopreservation, and were also establishable from the DF of already erupted wisdom teeth (Supplementary Fig. 1h).
The developed organoid structures displayed a dense morphology (Fig. 1a,b), showing an outer border of stratified cuboidal epithelium (CE) with cells displaying a high nucleo-cytoplasmic ratio, and an adjoining stratified squamous epithelium (SE; Fig. 1b). In DF tissue, epithelial cells with high nucleo-cytoplasmic ratio are present in the ERM (Supplementary Fig. 1c) [5]. In further analogy, the ERM markers cytokeratin (CK) 14 and CK5 [27] were detected in the organoids, as they are observed in compartments of the original DF tissue (Fig. 1c). Importantly, the mesenchymal (fibroblast) marker CD90 (Thy-1 cell surface antigen, THY1), which is observed in compartments of the original DF tissue was not detected in the organoids (Supplementary Fig. 1i), indicating the absence of pure mesenchymal cells in the (epithelial) organoids. Previous studies proposed that the ERM contains DESCs, among others marked by CD44 and P63 [12, 27, 28]. Interestingly, these markers, indeed observed in the primary DF tissue (Fig. 1d), were also detected in the derived organoids, both at initial formation (P0) and after passaging (P1; Fig. 1d). Moreover, the organoids and the native DF tissue expressed SOX2 (Fig. 1d), a well-known marker of DESCs in mouse [29,30,31,32] and detected in epithelium of developing teeth in humans [4]. Finally, the organoids showed prominent gene expression of the proposed ERM stem cell marker integrin-α6 (ITGα6) [8, 28], as well as of factors playing an important role in embryonic development of the dental epithelium (i.e. paired like homeodomain 2 (PITX2) [33] and bone-morphogenetic protein 4 (BMP4)) [34], all markers also detected in the original DF tissue (Supplementary Fig. 1j). Finally, we also observed expression of amelogenin (AMELX) in both primary tissue (more in particular in the CK5+ ERM region of the DF) and derived organoids (Fig. 1e). AMELX is not only a main constituent of enamel matrix but also a suggested marker of ERM cells (described in rat) [6, 35,36,37]. Proposed functions of this ERM-produced AMELX include maintenance of PDL space [38] and stimulation of cementoblast differentiation [39, 40]. Of note, AMELX localization can show a punctuated pattern, being present in vesicles and/or secreted in the extracellular space [41], which is also observed here (Fig. 1e).
Taken together, epithelial organoids can be established from human tooth-derived DF, displaying an ERM-mirroring, stemness expression phenotype and possessing robust long-term expandability.
Single-cell transcriptomics reinforces the organoid-ERM congruence
To decode the organoids in more granular detail, we applied single-cell RNA-sequencing (scRNA-seq) analysis on DF-derived organoids (at P1 and P4) together with their primary tissue (Fig. 2a; Supplementary Table 2). Unsupervised clustering of the aggregate data and visualization using uniform manifold approximation and projection (UMAP) [42] exposed main DF cell-type clusters annotated using reported markers [43] comprising immune, endothelial, mesenchymal and epithelial compartments, and grouped organoid clusters with noticeable overlap of the P1 and P4 cultures (Fig. 2a; Supplementary Fig. 2a; Supplementary Dataset 1). Of note, a cluster of lower quality cells (i.e. with low gene counts) was also discerned (Fig. 2a), not filtered out using the applied quality thresholds (see Methods; Supplementary Fig. 2b). This cluster likely comprises dying cells from the organoids’ core, as supported by gene ontology (GO) analysis [44] revealing an enriched ‘cell death’ biological term (Supplementary Fig. 2c), which conforms to ultrastructural features of apoptotic nuclei and absence of cell organelles in the organoids’ center (Supplementary Fig. 2c).
Compiling the clusters’ top 10 differentially expressed genes (DEGs; Supplementary Dataset 1) in a heatmap exposed specific expression patterns of the different clusters (Fig. 2b). Several of the reported stem cell and ERM markers (see above and [12, 27, 28, 33,34,35]) were found expressed in the organoids (Fig. 2c; Supplementary Fig. 2a,d), thus corroborating our observations above on organoid-ERM correspondence. Of note, mesenchymal markers such as fibroblast activation protein alpha (FAP) and collagen type I alpha 1 chain (COL1A1), being present in the DF mesenchymal (fibroblast) cluster, were not detected in the organoid clusters, thereby again demonstrating the absence of pure mesenchymal cells in the (epithelial) organoids (Supplementary Fig. 2d). In further analogy with the transcriptomic organoid-ERM congruence, gene-regulatory network (regulon) analysis using pySCENIC [45] (i.e. defining core transcription factors with their positively regulated target genes in single cells) showed high TP63 and PITX2 regulon activity in both organoids and ERM (Fig. 2d). Predicted target genes of the PITX2 regulon include SOX2, TP63, PITX2, KRT5, KRT14, and BMP4 (Fig. 2c). Interestingly, the newly proposed mouse incisor epithelial (stem) cell markers KRT15 and dentin sialoprotein (DSP) [46] were among the top 10 DEGs in the organoid as well as ERM clusters (Fig. 2b,c; Supplementary Fig. 2e). Moreover, high regulon activity of early growth response 1 (EGR1) (marking a transient progenitor population in mouse incisor [43]) was observed in organoid clusters and ERM, while substantial regulon activity of activating transcription factor 3 (ATF3) (recently reported as new mouse incisor epithelial cell marker [46]) also showed up in the organoid and ERM clusters (Fig. 2d). Together, our single-cell transcriptomic analysis uncovered new, mouse-mirroring molecular features for human ERM, at present ill-defined. Intriguingly, we also detected gene expression of the mesenchymal marker vimentin (VIM) in ERM cells (Fig. 2c; Supplementary Fig. 2a,e). In agreement, and in analogy with a previous report [47], we observed co-expression of VIM in ERM (CK5+) cells of the primary DF tissue (Fig. 2e), which was recapitulated in the initiating organoids (Fig. 2e; P0, day 7). These findings may point to a hybrid epithelial/mesenchymal (E/M) nature which has recently been correlated with stemness and active stem cells in several other (developing) tissues (such as human fetal pituitary, intestine, liver, lung) [48, 49]. VIM expression faded during further organoid culturing (Fig. 2e; day 14). However, VIM expression transiently reiterated at each passaging (as shown for P4; Fig. 2e), suggesting that the hybrid E/M (active stem cell) phenotype is re-activated at re-seeding. Taken together, our profound single-cell transcriptomic analysis further reinforces the congruence between the DF-derived organoids and the ERM (stem) cells residing in this tooth tissue. To finally corroborate this relationship, we isolated ERM stem cells from DF tissue by FACS based on ITGα6 expression ([8, 28] and Supplementary Fig. 2f), and seeded the cells in organoid-developing conditions. The ITGα6+ ERM cells formed organoids whereas ITGα6− cells did not (Supplementary Fig. 2f). Interestingly, the latter culture only showed mesenchymal cells adhering to the bottom of the culture plate which were not observed in the ITGα6+ cell culture (Supplementary Fig. 2f), further supporting that the DF’s mesenchymal cells do not contribute to, or make part of, organoid growth.
We further compared the organoids with the ERM by applying GO analysis. Negative regulation of ‘epithelial cell proliferation’, of ‘epithelial cell apoptotic process’ and of ‘epithelial cell differentiation’ are significantly enriched in the ERM versus the organoids (P1) (Fig. 2f; Supplementary Dataset 2, 3, 4a,b) which is in line with the quiescence stemness character of ERM under homeostatic conditions as reported before [5, 7, 12]. For instance, the cell cycle inhibitor CDKN1C is among the top DEGs in the ERM cluster (Fig. 2b). On the other hand, GO terms associated with cell cycle division are enriched in the organoids when compared to ERM (Fig. 2f; Supplementary Dataset 2, 3, 4c,d). In analogy, gene expression of the proliferation marker MKI67 is prominent in the organoid clusters and absent in the ERM cluster, further corroborated by immunostaining (Fig. 2b; Supplementary Fig. 2g). Also, other proliferation markers such as topoisomerase II alpha (TOP2A) and centromere protein F (CENPF) were found almost exclusively expressed in the organoid clusters (Supplementary Fig. 2g). Interestingly, CENPF was recently discovered in the dental epithelium of the continuously growing mouse incisor [46]. Together, these data provide supportive evidence that the ERM stem cells, being quiescent in vivo, are proliferatively (re-)activated in organoid culture.
In further GO analysis, we found that the biological terms ‘regulation of osteoblast differentiation’, ‘regulation of bone mineralization’ and ‘regulation of neuron projection development/neuron development’ are enriched in the ERM (Fig. 2f; Supplementary Dataset 2, 3, 4a,b), all representing previously proposed biological functions of this specific DF cell compartment [5, 26, 50]. Finally, the higher mesenchymal character of the ERM as compared to full-grown organoids (Fig. 2c,e) is reflected in the enriched ‘positive regulation of epithelial-mesenchymal transition’ (EMT) term (Fig. 2f), in agreement with higher regulon activity of the EMT-driving transcription factors TWIST1 and ZEB1 in the ERM (Supplementary Fig. 2h; Supplementary Dataset 2, 3, 4a,b).
In general, gene expression signatures of P1 and P4 organoids display substantial similarity (Fig. 2b; Supplementary Dataset 5). In particular, expression of stemness markers remains comparable after the additional passaging (Fig. 2g), indicating that the tooth organoids retain their stemness phenotype during expansive culture.
Taken together, our detailed scRNA-seq interrogation demonstrates and reinforces the organoid-ERM stemness relationship and uncovered new molecular fingerprints of human ERM, at present only poorly defined.
EGF induces a proliferative and EMT phenotype in tooth organoids, reminiscent of in vivo events in the ERM
To establish organoids from primary tissues, supplementation of EGF is generally found quintessential [13,14,15,16]. Hence, it is remarkable that DF-derived organoids develop and expand in the absence of exogeneous EGF (TOM; Supplementary Table 1). scRNA-seq mining exposed that the EGF receptor (EGFR) ligands amphiregulin (AREG) and heparin-binding EGF (HB-EGF), together with EGFR, are highly expressed in the organoids (Supplementary Fig. 3a) which may substitute for EGF. In line, blocking the EGFR with cetuximab (added at passaging) compromised organoid growth, resulting in smaller organoids (Supplementary Fig. 3b), thereby revealing the need for endogenous EGFR signaling.
In vivo, it is known that elevated EGF levels in the ERM compartment (as, for instance, occurring upon tooth movement, infection or trauma) activates ERM cell proliferation [5, 26, 51]. Supplementation of EGF (50 ng/ml) to initiating organoid cultures (medium referred to as TOM + EGF) resulted in an increased number of proliferating (KI67+) cells in the organoids (P0; day 7; Fig. 3a). The increase was swiftly followed by a decline in proliferation (day 14; Fig. 3a), coinciding with the induction of an EMT process, as supported by the emergence of VIM expression in the organoids’ border, found colocalized with epithelial CK5 or P63 in several cells (Fig. 3b; Supplementary Fig. 3c). Moreover, exposing the organoids to EGF after preceding growth and expansion in TOM led to a same process with appearance of VIM+ cells (Fig. 3c), as well as migration of cells out of the organoid structures to grow at the bottom of the culture plate, displaying mesenchymal (spindle-like) morphology and VIM expression (Fig. 3c). This motile behavior further underscores the occurrence of EMT, also supported by the upregulated expression of specific mesenchymal/EMT-linked factors (Supplementary Fig. 3d). It has been proposed that EMT induction in the ERM (as, for instance, occurring upon damaging tooth impact) enables the cells to migrate and eventually contribute to regeneration of neighboring tissues [5, 26, 47].
Taken together, adding EGF to the organoids recapitulates functional in vivo behavior of the ERM, thus advancing our new tooth organoid model as an interesting tool to study ERM phenotype and conduct, to date not well understood.
The tooth organoids are amenable to an ameloblast differentiation process
During tooth development, DESCs give rise to ameloblasts which produce enamel matrix proteins (EMPs) for amelogenesis [4, 52, 53]. It has also been shown that ERM can differentiate into ameloblast(-like) cells [6, 7] and produce EMPs [35, 54]. Ameloblast differentiation encompasses a secretory stage with production of the EMPs AMELX and ameloblastin (AMBN), and a maturation stage during which amelotin (AMTN) and odontogenic-ameloblast associated protein (ODAM) are produced [4, 35, 52, 53]. The EMPs are proteolytically cleaved by matrix metalloproteinase 20 (MMP20) and kallikrein (KLK4), typically expressed during the secretory and maturation phase, respectively. Here, we examined whether the DF-derived organoids, possessing an epithelial ERM-stemness phenotype, can be driven into differentiation toward ameloblasts.
Organoids expanded in TOM were switched to a medium previously reported to trigger ameloblast-like differentiation in 2D DESC cultures [41, 55, 56] (referred to as mineralization-inducing medium, MIM; Supplementary Table 3), and analyzed at multiple time points (Fig. 4a). Interestingly, ODAM expression swiftly emerged (from day 2) in the organoids switched to MIM, and increased in intensity while remaining absent in TOM-cultured organoids (Fig. 4a). AMELX protein, being already detected in standard TOM conditions (Fig. 1e), became visually more abundant in MIM-switched organoids from day 8, while remaining more constant in TOM-cultured organoids (Supplementary Fig. 4a). Time-lapse gene expression analysis showed that secretory-stage markers of amelogenesis [4, 35, 52, 53] mainly increased at day 5 in MIM, while maturation-stage markers [52, 53] peaked at day 8–14 (Fig. 4b). In contrast, expression did not change in TOM-cultured organoids (Supplementary Fig. 4b). In parallel with the differentiation process, the stemness phenotype of the organoids dropped showing a fast decline in SOX2+ and TP63+ cells (Fig. 4b). Interestingly, CK19 expression emerged in MIM-switched organoids (Fig. 4c), in accordance with the known gradual replacement of CK14 by CK19 in differentiating ameloblasts [57]. Moreover, the MIM-cultured organoids displayed calcium deposits (Fig. 4d; Alizarin red S (ARS) staining), supported by transmission electron microscopy (TEM) revealing the presence of electron-dense calcium-phosphate accumulations (Fig. 4d), analogous to the formation of hydroxyapatite during amelogenesis [53]. This mineralization outcome appeared even more prominent when the MIM-cultured organoids were incubated in an in vivo environment, i.e. following subcutaneous transplantation of 3D-printed hydroxyapatite scaffolds seeded with organoids in immunodeficient mice (Supplementary Fig. 4c; ARS and Masson’s trichrome staining (TCM)).
Finally, to validate whether the ameloblast differentiation capacity is already present in the organoid-initiating ERM stem cells, we developed the organoids immediately in MIM and subsequently analyzed their phenotype (in P1; Supplementary Fig. 4d). ODAM expression was detected in the MIM- (but not TOM-) grown organoids coinciding with the almost absence of SOX2+ cells. AMTN and KLK4 were also detected at higher levels in MIM- versus TOM-grown organoids, while MMP20 expression was only lowly expressed by both organoid types (Supplementary Fig. 4d).
Taken all together, our new tooth organoid model is capable of unfolding an ameloblast differentiation process involving known consecutive steps, thereby recapitulating DESC/ERM functionality, and thus provides a valuable research tool to study amelogenesis of human tooth, at present poorly defined.
Single-cell transcriptomics of tooth organoids enriches insights into amelogenesis
To decipher the amelogenesis differentiation process that occurs in the tooth organoids in deeper detail, we performed scRNA-seq analysis of P4 organoids switched to MIM for 8 days (referred to as P4-switch; see Fig. 4a), and integrated the data with the scRNA-seq dataset described above (Fig. 5a).
As expected, stemness markers (e.g. SOX2, KRT15) are more prominent in the non-differentiated P4 organoid cluster, whereas ameloblast differentiation markers (e.g. AMTN, ODAM) show almost exclusive expression in the differentiated P4-switch organoids (Fig. 5b), all concordant with the findings above. Analogously, the newly identified EGR1 and ATF3 are mainly expressed in the non-differentiated P4 organoid cluster, while the tooth development marker PITX2 was found in both stemness and differentiated organoid groups (Supplementary Fig. 5a).
Looking more broadly at gene expression differences using DEG analysis (Supplementary Dataset 6) revealed that P4 and P4-switch organoid clusters clearly display different gene signatures, thereby exposing interesting (new) markers (Fig. 5c). Among others, ornithine decarboxylase 1 (ODC1), a gene involved in cell cycle regulation [58] and proposed as a marker of dental epithelium (moreover aberrantly expressed in specific odontogenic tumors [58]), is higher expressed in P4 versus P4-switch organoids (Fig. 5c). In addition to KRT15 and ATF3 belonging to the top 10 upregulated DEGs in P4 organoids (Fig. 5c), other undifferentiated epithelial cell markers are also distinctly expressed in P4 versus P4-switch organoids, including death-associated protein-like 1 (DAPL1) and tissue inhibitor of metalloproteinases 1 (TIMP1) (Fig. 5c), both recently discovered markers of mouse dental epithelium [46, 59]. On the other hand, in addition to AMTN and ODAM surfacing in the top 10 DEGs of P4-switch as compared to P4 organoids (Fig. 5c; Supplementary Dataset 6), laminin subunit gamma 2 (LAMC2) and laminin subunit alpha 3 (LAMA3), both expressed in mature ameloblasts in mouse incisor [46] and essential for amelogenesis in humans (with mutations linked to amelogenesis imperfecta [60, 61]), are highly upregulated in P4-switch organoids (Fig. 5c), further validated by RT-qPCR (Supplementary Fig. 5b). Follicular dendritic cell secreted peptide (FDCSP), reported to bind to hydroxyapatite [62], is also distinctly expressed in the P4-switch organoids (Fig. 5c).
GO analysis revealed enriched ‘negative regulation of cell differentiation’ in the straight P4 organoids when compared to P4-switch organoids (Supplementary Fig. 5c; Supplementary Dataset 4e, 6). In the reverse comparison, GO analysis exposed enrichment of ‘epithelial cell differentiation’, ‘biomineral tissue development’, ‘odontogenesis’ and ‘calcium-mediated signaling’ in P4-switch versus P4 organoids (Fig. 5d; Supplementary Dataset 4f). Interestingly, also TGFβ-associated processes are upregulated (Fig. 5d), in line with the enrichment of ‘negative regulation of SMAD protein signal transduction’ in the non-differentiated organoids (Supplementary Fig. 5c), and the knowledge that the TGFβ pathway plays an important role in ameloblast differentiation [63].
Next, we performed gene set enrichment analysis (GSEA) [64] which exposed several important differentiation (amelogenesis) pathways in P4-switch versus P4 organoids. Firstly, mineralization hallmarks (tooth, enamel) are significantly enriched in P4-switch organoids (Fig. 5e). In addition, calcium-signaling pathways, highly important during amelogenesis [53], were found significantly associated with the P4-switch organoids such as the hallmarks ‘calmodulin binding’, ‘store-operated calcium entry’ and ‘calcium mediated signaling’ (Supplementary Fig. 5d). Further interestingly, GSEA revealed significant enrichment of TGFβ signaling hallmarks in P4-switch versus P4 organoids, more specifically TGFβ (receptor) signaling and TGFβ (particularly TGFβ1/3) production (Fig. 5e), in line with the importance of the TGFβ pathway in amelogenesis [63].
Regulon analysis exposed higher activity of the signal transducer and activator of transcription 2 (STAT2) gene-regulatory network in P4-switch than P4 organoids (Fig. 5f). STAT2 is specifically found in ameloblasts (reported in neonatal rat molars [65]) and positively targets AMTN, the ameloblast-related LAMC2 and LAMB3, as well as genes associated with TGFβ signaling (TGFβ3, TGFβR2) (Fig. 5f). Also, avian musculoaponeurotic fibrosarcoma (MAF), specifically expressed in ameloblasts (reported in mouse incisor tooth germs [66]) and representing an essential regulator of AMELX secretion during amelogenesis [66], shows higher regulon activity in P4-switch than P4 organoids (Fig. 5f). MAF is predicted to positively regulate fibronectin (FN1) and RUNX2, genes related to ameloblast differentiation, and TGFβ signaling-associated SMAD3, SMAD6, and TGFβ-induced (TGFβI), an activated form of the TGFβ1 ligand (Fig. 5f). RUNX2 expression has been reported in ameloblasts during the late secretory and maturation stages and its deletion suppresses enamel maturation [67]. In addition, TGFβ1 affects enamel mineralization by modulating RUNX2 [67, 68]. TGFβI is also an important predicted target gene of Forkhead Box C2 (FOXC2), and FOXC2 regulon activity was found higher in P4-switch than P4 organoids (Fig. 5f). FOXC2 is highly expressed during craniofacial development [69], but its exact role during tooth development and differentiation is unknown. FOXC2 is also predicted to positively regulate LAMC2 and MSX1, a highly conserved transcription factor well-known to regulate tooth formation [4], and causing tooth agenesis in humans when mutated [70]. Finally, SOX4 and HMGA2 regulons are prominently activated in P4-switch organoids (Supplementary Fig. 5e). SOX4 expression has been reported in DESCs and in inner enamel epithelium (at the cap stage in mouse) [71] and targets PITX2, while HMGA2 is involved in early tooth formation and stem cell marker (e.g. SOX2) expression [72] (hence, plausibly associated with enlarged and supernumerary teeth when truncated [72]), and predicted targets include FN1 and LAMA3 (Supplementary Fig. 5e).
Pseudotime trajectory analysis (using Monocle3 [73]) projected a potential developmental path from P1-P4 to P4-switch clusters (Supplementary Fig. 5f). Intriguingly, the trajectory passes through a particular subcluster of the P4-switch organoids (Supplementary Fig. 5f, encircled), likely representing a transitional stage as supported by the concurrent expression of stemness/development markers (SOX2, KRT15, PITX2) and differentiation markers (AMTN, ODAM) in this subcluster (see Fig. 5b; Supplementary Fig. 5a). Interestingly, regulons controlling ameloblast differentiation (PITX1, DLX3, MEIS1) are especially active in this subcluster (Supplementary Fig. 5g). PITX1 is required for proper tooth formation [74], and has been described in secretory stage ameloblasts [75]. DLX3 promotes the expression of EMPs during amelogenesis [76], and MEIS1 has been shown to bind to DLX3 [77].
In a final analysis of the scRNA-seq data, we applied STRING to in silico predict protein–protein interactions [78]. Using the top 40 DEGs in P4-switch versus P4 organoid clusters, it is projected that AMTN and ODAM closely interact (Supplementary Fig. 5h; Supplementary Dataset 6, 7a), in agreement with former reports [76]. Interestingly, AMTN is also predicted to cooperate with C4orf26, an ECM acidic phosphoprotein suggested to play a key role in enamel mineralization and crystal nucleation [76]. In addition, the STRING analysis proposed interaction of AMTN with LAMB3 suggesting a role in cell–matrix attachment, in line with previously proposed interactions of AMTN with laminins localized in the epithelial basement membrane of the ECM [76]. In the predicted network, LAMB3 interacts with LAMA3 and LAMC2 which is consistent with previous reports [76]. Interestingly, AMTN is also proposed to network with FN1, at present not reported. Moreover, FN1 is predicted to interact with TGFβ1 and ITGβ6. ITGβ6 is known to activate TGFβ1 by binding to arginine-glycine-aspartic acid (RGD) motifs present in ECM proteins such as FN1 [76]. GO-Biological Process analysis of these particular top 40 DEGs in P4-switch organoids confirmed key features of biomineral tissue development, odontogenesis and enamel mineralization, as well as of TGFβ signaling, the latter further stressed by KEGG pathway analysis (Supplementary Fig. 5i; Supplementary Dataset 7b,c).
Taken together, single-cell transcriptomics of the tooth organoids driven into amelogenesis differentiation demonstrates and underscores the relevance of our new organoid model by confirming known data as well as presenting new insights in the amelogenesis process in humans which is at present far from clarified.
TGFβ fortifies amelogenesis in tooth organoids and triggers PDL-like differentiation
The above analyses exposed the enrichment of TGFβ pathway processes in tooth organoids subjected to ameloblast differentiation, in line with the proposed key role of TGFβ in amelogenesis [63]. To assess the impact of the TGFβ pathway, we switched organoids grown in TOM (P5) to MIM with or without TGFβ (Fig. 6a). Immunofluorescence analysis revealed that addition of TGFβ further upregulated the expression of ODAM (Fig. 6a), supported by gene expression interrogation also showing increased expression of AMTN (Fig. 6b). The effect of TGFβ was blocked by the simultaneous addition of a TGFβ receptor inhibitor (LY2109761, further referred to as TGFβinh), thereby demonstrating the specificity of the effect observed (Fig. 6b). Intriguingly, adding TGFβinh to MIM-cultured organoids (i.e. without adding exogenous TGFβ) strongly reduced the upregulated ODAM and AMTN expression in MIM (Fig. 6b), indicating the presence and implication of endogenous TGFβ signaling in the differentiation process, in line with our findings above (Fig. 5d,e) and corroborated by the increase in expression of TGFβ1 and its receptors TGFβR1 and TGFβR2 in MIM culture (Supplementary Fig. 6a). Together, the data demonstrate that TGFβ further advances the amelogenesis-mimicking differentiation in the tooth organoids.
During tooth root development, Hertwig’s epithelial root sheath (HERS), from which ERM is eventually derived [4, 5], undergoes EMT to develop to participate in PDL formation [47]. It has previously been proposed that this EMT process is regulated by TGFβ, thereby triggering HERS/ERM cells to switch phenotype toward PDL cells [5, 47, 79]. Addition of TGFβ indeed further increased the expression of VIM in the epithelial (CK5+) organoids (Fig. 6a) and also significantly stimulated the expression of the PDL-specific genes periostin (POSTN) and collagen type III alpha 1 chain (COL3A1) [80] (Fig. 6c).
Taken together, TGFβ coerces the tooth organoids into more pronounced ameloblast differentiation as well as into the direction of PDL development. These findings conform to the known activity of TGFβ in these tooth developmental processes, and thus again corroborate the strength and validity of our new organoid model. Moreover, they provide supportive evidence that the DF-derived organoids replicate the multipotency of dental (HERS/ERM) epithelial stem cells as proposed to unfold in vivo during tooth development and possibly repair [5, 26].
The presence of tooth mesenchymal cells triggers ameloblast differentiation in the epithelial organoids
Given the importance of mesenchyme-epithelium interactions during tooth development including ameloblast differentiation/amelogenesis [4, 54], we addressed the question whether addition of dental mesenchymal cells had an impact on ameloblast differentiation of the epithelial organoids. We opted to use DPSCs to mimic early stages of tooth development in which DPSC-derived odontoblasts are in close contact with ameloblasts [4]. The DPSCs, isolated, grown and characterized using well-defined standard protocols [81,82,83], were combined with organoid-derived epithelial stem cells in a layered approach [20], thereby forming composite organoids (assembloids) which were cultured in a mixture of TOM and the DPSC growth medium αMEM (Fig. 6d). The hybrid epithelial-mesenchymal composition was confirmed by CK5-VIM immunofluorescence analysis (Fig. 6d), revealing VIM+ mesenchymal cells in the inner part and CK5+ epithelial cells at the outer zone of the assembloids (Fig. 6d), and by developing assembloids using eGFP-expressing DPSCs (Supplementary Fig. 6b).
Whereas ODAM is not present in the straight (pure) epithelial organoids cultured in TOM (see above and Supplementary Fig. 6c), it is expressed in the assembloids (Fig. 6d). This induction is not due to the addition of αMEM to TOM (Supplementary Fig. 6c). Interestingly, the epithelial cells neighboring the DPSCs express ODAM, whereas the cells at the outside border of the assembloids (thus, not in direct contact with the mesenchymal cells while more exposed to the (stem cell) medium) do not (Fig. 6d). These findings indicate that the presence of (and even more, the close interface with) mesenchymal (stem) cells drives epithelial stem cells into ameloblast differentiation. Addition of TGFβinh completely abolished ODAM protein expression in the assembloids (Fig. 6d) and reduced the expression of AMTN (Supplementary Fig. 6d), indicating the involvement of endogenous TGFβ signaling in the observed effects. TGFβ pathway components are indeed expressed in the assembloid culture (Supplementary Fig. 6e); the ligand(s) may originate from the epithelial cells (see Supplementary Fig. 6a), further upregulated by the presence of mesenchymal cells, or may be additionally produced by the mesenchymal cells since both dental cell types have been shown to produce TGFβ [84, 85].
Taken together, ameloblast differentiation of epithelial (organoid) stem cells is triggered by the presence of tooth mesenchymal cells involving TGFβ signaling, thereby corroborating in vivo findings of interactive mesenchyme-epithelium importance, and further validating our model as valuable research tool for exploring human tooth (stem cell) biology.