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gga-miR-142-3p negatively regulates Mycoplasma gallisepticum (HS strain)-induced inflammatory cytokine production via the NF-κB and MAPK signaling by targeting TAB2

  • Original Research Paper
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Abstract

Objective

Mycoplasma gallisepticum (MG), a notorious avian pathogen, leads to considerable economic losses in the poultry industry. MG infection is characterized by severe, uncontrollable inflammation and host DNA damage. Micro ribonucleic acids (miRNAs) have emerged as important regulators in microbial pathogenesis. However, the role of miRNAs in MG infection is poorly characterized. In this study, we validated the functional roles of gga-miR-142-3p.

Methods

The relative expression of gga-miR-142-3p in the lungs of the MG-infected chicken embryos and the MG-infected chicken embryonic fibroblast cell line (DF-1) was determined by reverse transcription quantitative real-time PCR analysis. Bioinformatics database was used to analysis the target gene of gga-miR-142-3p. The luciferase reporter assay as well as gene expression analysis were conducted to validate the target gene. To further explore the biological functions of gga-miR-142-3p upon MG infection, the cell proliferation was quantified using Cell Counting Kit-8 (CCK-8). Meanwhile, cell cycle analysis and apoptosis were measured using a flow cytometer.

Results

gga-miR-142-3p was significantly upregulated in both MG-infected chicken-embryo lungs and the DF-1 cells. gga-miR-142-3p over expression significantly downregulated the expression of pro-inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor alpha after MG infection. Meanwhile, gga-miR-142-3p enhanced the host defense against MG infection by facilitating cell proliferation, promoting cell progression and inhibiting cell apoptosis. Interestingly, TAB2 knockdown groups show similar results, whereas, TAB2 over-expression groups and gga-miR-142-3p inhibitor groups had thoroughly opposite results. The expression of p-p65 in nuclear factor kappa B (NF-κB) and p-p38 in the mitogen-activated protein kinase (MAPK) pathway was decreased when gga-miR-142-3p was over-expressed.

Conclusion

Upon MG infection, upregulation of gga-miR-142-3p alleviates inflammation by negatively regulating the signaling pathways of NF-κB and MAPKs by targeting TAB2 and facilitates cell proliferation by inhibiting cell apoptosis and promoting cell cycle progression to defend against MG infection.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 31972681) and the Fundamental Research Funds for the Central Universities (No. 2662017PY080).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Ministry of Education, College of Animal Science and Technology and College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

    Yaping Yang, Yingjie Wang, Mengyun Zou & Xiuli Peng

  2. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yaping Yang & Ganzhen Deng

Authors
  1. Yaping Yang
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Contributions

YY and XP designed the project and experiments. GZ and MZ carried out most of the experiments. YW carried out statistical analysis and prepared the figures.

Corresponding author

Correspondence to Xiuli Peng.

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Conflict of interest

Yaping Yang declares that he has no conflict ofinterest. Yingjie Wang declares that he has no conflict of interest. Mengyun Zou declares that he has no conflict of interest. Ganzhen Deng declares that he has no conflict of interest. Xiuli Peng declares that she has no conflict of interest.

Ethics statement

Our experimental protocols for chicken-embryo treatment were authorized by the Ethical Committee on Animal Research at Huazhong Agricultural University (HZAUMO-2015–12). Theses producers were operated in accordance with the approved rules.

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The authors confirm that all applicable international, national, and/or institutional guidelines for the care and use of animals were followed in this research and in the preparation of this manuscript.

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Yang, Y., Wang, Y., Zou, M. et al. gga-miR-142-3p negatively regulates Mycoplasma gallisepticum (HS strain)-induced inflammatory cytokine production via the NF-κB and MAPK signaling by targeting TAB2. Inflamm. Res. 70, 1217–1231 (2021). https://doi.org/10.1007/s00011-021-01499-2

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  • DOI: https://doi.org/10.1007/s00011-021-01499-2

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