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Comparison of the endogenous heptapeptide Met-enkephalin-Arg6-Phe7 binding in amphibian and mammalian brain

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Abstract

In previous communications [4, 38] we published that [3H]Met-enkephalin-Arg6-Phe7 (MERF) binds to opioid (kappa2 and delta) and sigma2 sites in frog and rat brain membrane preparations, however no binding to kappa1 sites could be established. In the present paper we compare the frog, rat and guinea pig brain membrane fractions with respect to their MERF binding data. No qualitative differences were found between the three species but specific binding of labelled MERF was maximal in frog brain and lowest in guinea pig brain, which corresponds to their kappa2 opioid receptor distribution. The naloxone resistant binding was also present in all investigated species and varied from 25% in frog and guinea pig cerebrum, to 50% in rat cerebrum and cerebellum, but no naloxone inhibition was found in guinea pig cerebellum where no kappa2 opioid receptors have been found. The presence of sigma2-like receptor was demonstrated in each investigated membrane fraction with displacement experiments using (–)N-allyl-normetazocine as competitor of tritiated MERF. It was shown that this site was responsible for 60–80% of [3H]MERF binding. The remaining part of the naloxone resistant labelled MERF binding could be displaced only with endogenous opioid peptides as met-enkephalin, dynorphin and ß-endorphin. The eventual physiological role of multiple MERF receptors is discussed.

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Authors and Affiliations

  1. Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6701, Szeged, P.O. Box 521, Hungary

    Mária Wollemann, Judit Farkas, G. Tóth & S. Benyhe

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  1. Mária Wollemann
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  2. Judit Farkas
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  3. G. Tóth
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  4. S. Benyhe
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Dedicated to Professor János Salánki for his 70th birthday.

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Wollemann, M., Farkas, J., Tóth, G. et al. Comparison of the endogenous heptapeptide Met-enkephalin-Arg6-Phe7 binding in amphibian and mammalian brain. BIOLOGIA FUTURA 50, 297–307 (1999). https://doi.org/10.1007/BF03543051

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