Abstract
The aim of the present study was to identify SCAR (sequence characterised amplified regions) markers linked to oat mildew resistance group 2 (OMR2). The bulked segregant analysis was used to determine RAPD markers linked to OMR2. Four products were identified that were able to differentiating between resistant and susceptible plants. The products were cloned and sequenced and primers for PCR were designed. Our examinations showed that only the BG8 marker was linked to OMR group 2 at a distance of 4.5 cM from the analysed region. The obtained SCAR-BG8 marker might be used to select genotypes that are resistant to powdery mildew and that contain OMR2.
This is a preview of subscription content, log in via an institution to check access.
References
Chen G, Chong J, Prashal S & Procunier JD, 2007. Discovery and genotyping of high-throughput SNP markers for crown rust resistance gene Pc94 in cultivated oat. Plant Breeding 126, 379–384.
Chong J, Reimer E, Somers D, Aung T & Penner GA, 2004. Development of sequence-characterized amplified region (SCAR) markers for resistance gene Pc94 to crown rust in oat. Can J Plant Pathol 26, 89–96.
Clifford BC, 1995. Diseases, pest and disorders of oat. In: RW Welch (Ed), The Oat Crop, Chapman & Hall, London: 252–278.
Hsam SLK, Pederina E, Gorde S & Zeller FJ, 1998. Genetic studies of powdery mildew resistance in cultivated oat (Avena sativa L.). II. Cultivars and breeding lines grown in Northern and Eastern Europe. Hereditas 129, 227–230.
Hsam SLK, Peters N, Paderina EV, Felsenstein F, Oppitz K & Zeller FJ, 1997. Genetic studies of powdery mildew resistance in common oat (Avena sativa L.). I. Cultivars and breeding lines grown in Western Europe and North America. Euphytica 96, 421–427.
Jones IT & Jones ERL, 1979. Mildew of oats. UK Cereal Pathogen Virulence Survey 1978. Annual Rep, 59–63.
Kulcheski FR, Graichen FAS, Martinelli JA, Locatelli AB, Federizzi LC, Delatorre CA, 2010. Molecular mapping of Pc68, a crown rust resistance gene in Avena sativa. Euphytica 175, 423–432.
Michelmore RW, Paran I & Kesseli RV, 1991. Identification of markers linked to disease-resistance genes by bulked segregants analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88, 9828–9832.
Okoń S & Kowalczyk K, 2012. Deriving isolates of powdery mildew in common oat and using them to identify selected genes of resistance. Acta Agrobot 65, 155–160.
Tanhuanpää P, Kalendar R, Laurila J, Schulman AH, Manninen O & Kiviharju E, 2006. Generation of SNP markers for short straw in oat (Avena sativa L.). Genome 49, 282–287.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SV, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 18, 6531–6535.
Yu J & Herrmann M, 2006. Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat. Theor Appl Genet 113, 429–437.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Okon, S., Kowalczyk, K. Identification of SCAR markers linked to resistance to powdery mildew in common oat (Avena sativa). J Plant Dis Prot 119, 179–181 (2012). https://doi.org/10.1007/BF03356439
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03356439