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Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells

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Abstract

We studied whether a novel vasoconstrictor, endothelin-1 (ET-1), is synthesized by and released from porcine granulosa cells, and whether ET-1 acts directly on granulosa cells in an autocrine/paracrine fashion. The dilution curve of the conditioned medium from cultured porcine granulosa cells was parallel to a standard curve of ET-1 in RIA. Reverse-phase HPLC of the conditioned media from the granulosa cells revealed a major peak of ET-1-like immunoreactivity (ET-1-LI) coeluting with standard ET-1. ET-1-LI was released from cultured porcine granulosa cells as a function of time. Northern blot analysis demonstrated the expression of mRNA for prepro-ET-1 in the granulosa cells. We demonstrated the presence of ET-1 in the follicular fluid of porcine ovaries, and that the concentration of ET-1 in large follicles was higher than that in small-medium follicles. ET-1 dose-dependently stimulated cell growth and DNA synthesis in porcine granulosa cells. ET-1 inhibited the FSH- and hCG stimulated accumulation of progesterone in porcine granulosa cells in long-term incubation. These findings suggest that ET-1 produced by porcine granulosa cells may function as an autocrine/paracrine growth factor and modulator of steroidogenesis in ovarian granulosa cells.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan

    Shusaku Kamada M.D., T. Kubota, M. Taguchi & T. Aso

  2. Second Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Tokyo, Japan

    Y. Hirata, T. Imai, K. Ohta & F. Marumo

Authors
  1. Shusaku Kamada M.D.
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  2. T. Kubota
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  3. Y. Hirata
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  4. T. Imai
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  5. K. Ohta
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  6. M. Taguchi
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  7. F. Marumo
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  8. T. Aso
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Kamada, S., Kubota, T., Hirata, Y. et al. Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells. J Endocrinol Invest 16, 425–431 (1993). https://doi.org/10.1007/BF03348872

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  • DOI: https://doi.org/10.1007/BF03348872

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