Abstract
Protoplasts were isolated from the basal meristematic region of leaves from 6-day-old seedlings of wheat (Triticum aestivum). Protoplasts divided when cultured on MS medium (as agarose beads) in presence of nurse tissue. Donor seedlings when grown on BAP-supplemented MS medium were found to be considerably superior for protoplast isolation and culture than when grown on MS basal medium, in terms of protoplast viability, cell wall formation and cell division frequency. In addition, reduction of ammonium content of the culture medium, together with a dark Incubation, led to a high protoplast division frequency of about 70%. Microcolonies of 10-to 12-celled stages were obtained in Triticum aestivum, varieties HD 2329, HD 2285, Kalyan Sona, Arjun and CPAN 1676.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Vasil V, Redway F & Vasil IK, Bio/Technology, 8 (1990) 429.
Qiao Y-M, Cattaneo M, Locatelli F & Lupotto E, Plant Cell Rep, 11 (1992) 262.
Li ZY, Xia GM & Chen HM, Plant Cell, Tissue & Org Cult, 28 (1992) 79.
Harris R, Wright M, Byrne M, Varnum J, Brightwell B & Schubert K, Plant Cell Rep, 7 (1988) 337.
Wang WC & Nguyen H, Plant Cell Rep, 8 (1990) 639.
He DG, Yang YM & Scott KJ, Plant Cell Rep, 11 (1992) 16.
Chang YF, Wang WC, Warfield CY, Nguyen HT & Wong JR, Plant Cell Rep, 9 (1991) 611.
Shillito RD, Carswell GK, Johnson CM, Di Maio JJ & Harms CT, Bio/Technology, 7 (1989) 581.
Lührs R & Lörz H, Planta, 175 (1988) 71.
Potrykus I, Bio/Technology, 8 (1990) 535.
Ahuja PS, Pental D & Cocking EC, Z Pflanzenzüchtg, 89 (1982) 139.
Zamora AB & Scott KJ, Plant Sci Lett, 29 (1983) 183.
Wernicke W & Milkovits L, J Plant Physiol, 115 (1984) 49.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Frearson EM, Power JB & Cocking EC, Dev Biol, 33 (1973) 130.
Hahne B, Fleck J & Hahne G, Proc Natl Acad Sci, USA, 86 (1989) 6157.
Li XH, Van QS, Huang MJ, Sun YR & Li WB, In Advances in protoplast research. (L Ferenczy, GL Farkas, Editors), Pergamon Press, Oxford (1980) pp 261–267.
Bandyopadhyay S & Ghosh PD, Curr Sci, 55 (1986) 1084.
Malik KA & Saxena PK, Naturwissenschaften, 79 (1992) 136.
Kao KN & Michayluk MR, Planta, 126 (1975) 105.
Maheshwari SC, Gill R, Maheshwari N & Gharyal PK, In Results and problems in cell differentiation, Vol. 12. Differentiation of protoplasts and of transformed plant cells, (J Reinert, H Binding, Editors), Springer-Verlag, Berlin, Heidelberg (1986) pp 3–36.
Rajyalakshmi K, Dhir SK, Maheshwari N & Maheshwari SC, Plant Breed, 101 (1988) 80.
Maheshwari N, Rajyalakshmi K, Chowdhry CN, Grover A, Tyagi AK & Maheshwari SC, In The impact of biotechnology in agriculture, (RS Sangwan, BS Sangwan-Norreel, Editors). Kluwer Academic Publishers, Dordrecht (1989) pp 191–213.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mahalakshmi, A., Maheshwari, S.C. & Khurana, P. High Frequency Divisions in Leaf Base Protoplasts of Wheat (Triticum aestivum L.). J. Plant Biochem. Biotechnol. 2, 61–65 (1993). https://doi.org/10.1007/BF03262924
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03262924