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Gene mapping related to yellow green leaf in a mutant line in rice (Oryza sativa L.)

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Abstract

A mutant, which derived from the restorer line Jinhui10 treated with EMS, showed completely yellow green leaves, and it had low chlorophyll content and poor agronomic characteristics during the growing stage. The F1 plants from the cross between normal × the mutant showed normal green leaves, and the segregation ratio of normal to yellow green leaves was 3 : 1 in F2 population. It indicated that the trait was controlled by a single recessive nuclear gene, temporarily designated asygl3. The geneygl3 was mapped between RM468 and RM3684 with genetic distances 8.4 cM and 1.8 cM on chromosome 3. This result would be used as genetic information for fine mapping and map-based cloning ofygl3 gene.

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References

  • Ayumi T andRyouichi T (2006) Chlorophyll metabolism. Curr. Opin. Plant Biol. 9: 248–255.

    Article  Google Scholar 

  • Beale SI (2005) Green genes gleaned. Trend Plant Sci. 10: 309–312.

    Article  CAS  Google Scholar 

  • Braun DM, Ma Y, Inada N, Muszynski MG andBaker RF (2006) Tie-dyed1 regulates carbohydrate accumulation in maize leaves. Plant Physiol. 142: 1511–1522.

    Article  CAS  PubMed  Google Scholar 

  • Davison PA, Schubert HL, Reid JD, Iorg CD, Herou XA, Hill CP andHunter CN (2005) Structural and biochemical characterization of GUN4 suggests a mechanism for its role in chlorophyll biosynthesis. Biochemistry 44: 7603–7612.

    Article  CAS  PubMed  Google Scholar 

  • Dong FG, Zhu XD, Xiong ZM, Cheng SH, Sun ZX andMin SK (1995) Breeding of a photo-thermoperiod sensitive genetic male sterile indica rice with a pale-green-leaf marker. Chin. J. Rice Sci. 9: 65–70.

    CAS  Google Scholar 

  • Falbel TG, Meehl JB andStaehelin LA (1996) Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis. Plant Physiol. 110: 821–832.

    Article  Google Scholar 

  • Hirochika H, Guiderdoni E, An G, Hsing YI, Eun MY, Han CD, Upadhyaya N, Ramachandran S, Zhang Q, Pereira A, Sundaresan V, Leung H andAn G (2004) Rice mutant resources for gene discovery. Plant Mol. Biol. 54: 325–334.

    Article  CAS  PubMed  Google Scholar 

  • Huang XQ, Wang PY, Zhao HX andDong XJ (2007) Genetic analysis and molecular mapping of a novel chlorophyll-deficit mutant gene in rice. Chin. J. Rice Sci. 21: 355–359.

    CAS  Google Scholar 

  • Iwata N andSatoh H (1977) Linkage studies in rice Linkage groups for 6 genes newly described. Japan J. Breed 27: 250–251.

    Google Scholar 

  • Jung KH, Hur J, Ryu CH, Choi Y, Chung YY, Miyao A, Hirochika H andAn G (2003) Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol. 44: 463–472.

    Article  CAS  PubMed  Google Scholar 

  • Krause GH andWeis E (1991) Chlorophyll fluorescence and photosynthesis: the basis. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 313–349.

    Article  CAS  Google Scholar 

  • Kurata N, Miyoshi K, Nonomura KI, Yamazaki Y andIto Y (2005) Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol. 46: 48–62.

    Article  CAS  PubMed  Google Scholar 

  • Lander ES, Green P andAbrahamson J (1987) MAPMARKER: aninteractive computer for constructing primary genetics linkage maps of experimental and natural populations. Genetics 1: 174–182.

    CAS  Google Scholar 

  • Larkin RM, Alonso JM, Ecker JR andChory J (2003) GUN4, a regulator of chlorophyll synthesis and intracellular signaling. Science 299: 902–906.

    Article  CAS  PubMed  Google Scholar 

  • Lee S, Kim JH, Yoo E, Lee CH, Hirochika H andAn G (2005) Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol. Biol. 57: 805–818.

    Article  CAS  PubMed  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol. 48: 350–382.

    Article  Google Scholar 

  • Luo ZK, Yang ZL, Zhong BQ, Li YF, Xie R, Zhao FM, Ling YH andHe GH (2007) Genetic analysis and fine mapping of a dynamic rolled leaf gene,RL10(t), in rice (Oriza sativa L.). Genome 50: 811–817.

    Article  CAS  PubMed  Google Scholar 

  • Meinke D andKoornneef M (1997) Community standards forArabidopsis genetics. Plant J. 12: 247–253.

    Article  CAS  Google Scholar 

  • Miura E, Kato Y, Matsushima R, Albrecht V, Laalami S andSakamoto W (2007) The Balance between Protein Synthesis and Degradation in Chloroplasts Determines Leaf Variegation in Arabidopsis. Plant Cell 19: 1313–1328.

    Article  CAS  PubMed  Google Scholar 

  • Mochizuki N, Brusslan JA, Larkin R, Nagatani A andChory J (2001) Arabidopsis genomes uncoupled 5(GUN5) mutant reveals the involvement of Mgchelatase H subunit in plastid-to-nucleus signal transduction. Proc. Natl. Acad. Sci. USA 98: 2053–2058.

    Article  CAS  PubMed  Google Scholar 

  • Murray MG andThompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucl. Acids. Res. 8: 4321–4325.

    Article  CAS  PubMed  Google Scholar 

  • Nagata N, Tanaka R andSatoh S (2005) Identification of vinyl reductase gene for chlorophyll synthesis in Arabidopsis thanliana and implications for the evolution of Prochlorococcus Species. Plant Cell 17: 233–240.

    Article  CAS  PubMed  Google Scholar 

  • Nothnagel T, Ahne R andStraka P (2003) Morphology, inheritance and mapping of a compressed lamina mutant of carrot. Plant Breeding 122: 339–342.

    Article  CAS  Google Scholar 

  • Panaud O, Chen X andMcCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L). Mol. Gen. Genet. 259: 597–607.

    Google Scholar 

  • Sang XC, Yang ZL, Zhong BQ, Lin YF, Hou L, Pei Y, Li GY andHe GH (2006) Assessment of purity of rice CMS lines using cpDNA marker. Euphytica 152: 177–183.

    Article  CAS  Google Scholar 

  • Sato Y, Morita R, Nishimura M, Yamaguchi H andKusaba M (2007) Mendel’s green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway. Proc. Natl. Acad. Sci. USA 104: 14169–14174.

    Article  CAS  PubMed  Google Scholar 

  • Stern DB, Hanson MR andBarkan A (2004) Genetics and genomics of chloroplast biogenesis: Maize as a model system. Trend Plant Sci. 9: 293–301.

    Article  CAS  Google Scholar 

  • Wang J, Wang BH, Zhou LH, Xu JF, Gu MH andLiang GH (2006) Genetic analysis and molecular mapping of a new yellow-green leaf gene ygl-2 in rice. Chin. J. Rice. Sci. 20: 455–459.

    CAS  Google Scholar 

  • Wu DX, Shu QY andXia YW (2002) In vitro mutagenesis induced novel thermo / photoperiod-sensitive genic male sterile indica rice with green-revertible xanthan leaf color marker. Euphytica 123: 195–202.

    Article  Google Scholar 

  • Wu ZM, Zhang X, He B, Diao LP, Sheng SL, Wang JL, Guo XP, Su N, Wang LF, Jiang L, Wang CM, Zhai HQ andWan JM (2007) A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol. 145: 29–40.

    Article  CAS  PubMed  Google Scholar 

  • Zhang HT, Li JJ, Yoo JH, Yoo SC, Cho SH, Koh HJ, Seo HS andPeak NC (2006) Rice chlorina-1 and chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development. Plant Mol. Biol. 62: 325–337.

    Article  CAS  PubMed  Google Scholar 

  • Zhao Y, Wang ML, Zhang YZ, Du LF andPan T (2000) A chlorophyll-reduced seedling mutant in oilseed rape, Brassica napus, for utilization in F1 hybrid production. Plant Breeding 119: 131–135.

    Article  Google Scholar 

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Authors and Affiliations

  1. Rice Research Institute, Key Lab of Biotechnology and Crop Quality Improvement of the Agricultural Ministry, Southwest University, 400715, Chongqing, P. R. China

    Peng Du, Ying-Hua Ling, Xian-Chun Sang, Fang-Ming Zhao, Zheng-Lin Yang & Guang-Hua He

  2. Rice and Sorghum Institute, Sichuan Academy of Agricultural Sciences, Luzhou, 646000, Sichuan, P. R. China

    Rong Xie

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  1. Peng Du
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  2. Ying-Hua Ling
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  3. Xian-Chun Sang
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  4. Fang-Ming Zhao
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  5. Rong Xie
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  6. Zheng-Lin Yang
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  7. Guang-Hua He
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Correspondence to Guang-Hua He.

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Du, P., Ling, YH., Sang, XC. et al. Gene mapping related to yellow green leaf in a mutant line in rice (Oryza sativa L.). Genes & Genomics 31, 165–171 (2009). https://doi.org/10.1007/BF03191149

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  • DOI: https://doi.org/10.1007/BF03191149

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