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Induction of cytochrome P-450 isozymes upon repeated administration of nifedipine

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Summary

In experiments on male Wistar rats it has been found that nifedipine administration at a dose of 10 mg/kg body weight i.p. daily for 20 days did not significantly increase the total amount of cytochrome P-450 but markedly increased the 7α-, 16β- and 6β-hydroxylation of androstendione in liver microsomes, suggesting the induction of cytochromes P-450HA1, P-450UB1, and P-450IQA1, respectively. The induction of cytochrome P-450IHB1 was also confirmed immunochemically with polyclonal antibodies against cytochrome P-450HB1/B2.

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Abbreviations

AD:

androstendione

HPLC:

high performance liquid chromatography

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Authors and Affiliations

  1. Institute of Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

    M. Koleva & Ts. Stoytchev

  2. Institute of Clinical & Experimental Medicine, Siberian Division of USSR Academy of Medical Sciences, Timakova Str. 2, 630117, Novosibirsk, USSR

    L. Gulyaeva, A. Grishanova, V. Mishin & V. Lyakhovtch

Authors
  1. M. Koleva
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  2. Ts. Stoytchev
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  3. L. Gulyaeva
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  4. A. Grishanova
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  5. V. Mishin
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  6. V. Lyakhovtch
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Additional information

TheNomenclature System of Nebert et al. (1) is used throughout this paper to designate cytochrome P-450 forms.

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Koleva, M., Stoytchev, T., Gulyaeva, L. et al. Induction of cytochrome P-450 isozymes upon repeated administration of nifedipine. European Journal of Drug Metabolism and Pharmacokinetics 16, 103–106 (1991). https://doi.org/10.1007/BF03189945

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  • DOI: https://doi.org/10.1007/BF03189945

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