Abstract
The objective of this study was to analyze the proliferative response of BALB/c mice lymphocytes after in vitro irradiation (0.05 to 6 Gy). The capability of irradiated lymphocytes for proliferating without any stimulation and after activation with specific T and B cell mitogens has been evaluated. The results show that ionizing radiation significantly inhibits spontaneous cellular proliferation and that induced by mitogens and that variations in the degree of inhibition are found depending on the inducing proliferation mitogens and the dosage applied. The conclusion drawn is that different lymphocyte populations have different radiosensitivities, being B cells more sensitive to ionizing irradiation than T cells. Besides, the effects of gamma-irradiation vary according to the different subpopulations of T cells or, alternatively, to different T-dependent activation mechanisms.
Resumen
El objetivo de este estudio fue analizar la respuesta proliferativa de linfocitos de ratón BALB/c después de irradiación in vitro (0.05 to 6 Gy). Se evaluó la capacidad de los linfocitos irradiados para proliferar sin estímulo y también tras la activación con mitógenos específicos para las células T y B. Los resultados mostraron que la radiación ionizante inhibe significativamente la proliferación celular espontánea y la inducida por mitógenos y que las variaciones en el grado de inhibición son dependientes del mitógeno inductor de la proliferación y de la dosis aplicada. Se concluye que las diferentes poblaciones linfocitarias tienen diferentes radiosensibilidades. Así, las células B son más sensibles a la radiación ionizante que las células T. Por otra parte, los efectos de la radiación gamma varían según diferentes subpoblaciones de células T o, alternativamente, según diferentes mecanismos de activación T-dependientes.
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Hernández-Godoy, J., Planelles, D., Balsalobre, B. et al. The effect of in vitro γ-irradiation on mitogenic responsiveness of murine lymphocytes. J Physiol Biochem 64, 179–187 (2008). https://doi.org/10.1007/BF03178840
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DOI: https://doi.org/10.1007/BF03178840