Abstract
Hypocotyl and stem explants of 12 cultivars of mothbean callused and differentiated embryoids/shoot buds on basal medium devoid of growth regulators. Cotyledon and leaf explants failed to differentiate. Factors that influence differentiation of embryoids/shoot buds such as pre-conditioning of the germinating seeds with cytokinins and antiauxins, size and orientation of the explant on the medium and use of thin-layer peelings as explant were investigated. The shoot buds/embryoids developed into full fledged plantlets with roots on the same medium and were reared to maturity in field.
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Gill, R., Eapen, S. & Rao, P.S. Tissue culture studies in mothbean — Factors influencing plant regneration from seedling explants of different cultivars. Proc. Indian Acad. Sci. 96, 55–61 (1986). https://doi.org/10.1007/BF03053271
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DOI: https://doi.org/10.1007/BF03053271