Abstract
Callus cultures were initiated from immature embryos and seeds of bread wheat. The frequency and intensity of callus development were found to be best when embryos were oriented with the scutellum facing up and the embryonal axis in contact with the medium. Callusing frequency was also enhanced when embryos, were cut longitudinally into two halves and cultured. Agar-solidified medium produced a better frequency of, embryo response compared to stationary liquid cultures. Increasing levels of glucose and sucrose caused a decrease in fresh weight and a sharp increase in dry weight. Addition of coconut water, yeast extract, malt extract, amino acids and triacontanol was either without effect or were inhibitory for growth of callus. Histology of regeneration from embryo-derived callus showedde novo differentiation of shoot buds and roots. No evidence for somatic embryogenesis was observed.
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Eapen, S., Rao, P.S. Factors controlling callus initiation, growth and plant regeneration in breadwheat (Triticum aestivum L). Proc. Indian Acad. Sci. 94, 33–40 (1985). https://doi.org/10.1007/BF03053103
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DOI: https://doi.org/10.1007/BF03053103