Summary
Tissue cultures derived from the scutellum of immature embryos ofTriticum aestivum L. (wheat) gave rise to somatic embryos with a well-defined scutellum and coleoptile as well as one or more shoot primordia and a root primordium. The normal somatic embryos were formed from compact, white callus tissue which was not observed until 4 or more weeks after culture initiation. The highest frequency of white embryogenic tissue formation and the most normal embryoids were obtained in the dark on Murashige and Skoog's medium with twice the concentration of inorganic salts plus sucrose (2%), inositol (100–200 mg/l), casein hydrolysate (100–200 mg/l), glutamine (500 mg/l), and 2,4-dichlorophenoxyacetic acid (1–2 mg/l).
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Ozias-Akins, P., Vasil, I.K. Improved efficiency and normalization of somatic embryogenesis inTriticum aestivum (wheat). Protoplasma 117, 40–44 (1983). https://doi.org/10.1007/BF01281782
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DOI: https://doi.org/10.1007/BF01281782