Abstract
The LC50 values for 4, 5 and 7 day old larvae ofSpodoptera litura were respectively, 4·677, 13·740 and 37·240×106 polyhedral inclusion bodies (PIB)/ml. The adjusted relative susceptibility on the basis of relative amounts of food consumed by the various age group of larvae was found to be 1, 10·8 and 88·5 for 7, 5 and 4 day old larvae. The LT50 values ranged from 5·32 to 9·50 days for the various concentrations of poly-hedra/ml. Activated charcoal and camel special water proof black drawing ink protected the virus to a certain extent against ultraviolet light.
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References
Hunter, D. K. and Hall, I. M., Pathogenicity of a nuclear polyhedrosis virus of the beet army worm,spodoptera exigua.J. Invertebr. Pathol. 10 83–85 (1968).
Ignotho, C. M., Bioassay technique and pathogenicity of nuclear polyhedrosis virus of the cabbage looper,Trichopluria ni.J. Insect Pathot,6 237–245 (1964).
Ignoffo, C. M., The nuclear polyhedrosis virus ofHeliothis zea (Boddie) andHeliothis virescens (Fabricius). IV. Bioassay of virus activity.J. Invertebr. Pathol. 7 315–316 (1965).
Doane, C. C., Pathologens of the gypsy moth.Proc. Internat. Collq. on Insect Pathology and Microbial Control, Wageningen, The Netherlands, 200–203 (1966).
Pawar, V. M., Investigations on the nuclear polyhedrosis ofSpodoptera litura (Fabricius). Post Graduate School, Indian Agricultural Research Institute, New Delhi (M.Sc. Thesis) 76 pages (1970).
Jaques, R. P., Tests on protectants for foliar deposits of a polyhedrosis virus,J. Invertebr. Pathol. 17 9–16 (1971).
Morris, O. N., The effect of sunlight, ultraviolet and gamma radiation and temperature on the infectivity of a nuclear polyhedrosis virus.J. Invertebr. Pathol. 18 292–294 (1971).
Morris, O. N., Quantitative infectivity studies on the nuclear polyhedrosis of the Western oak looper,Lambdina fiscellaria somniaria Hulst.J. Insect Pathol. 4 207–215 (1962).
Smirnoff, W. A., The effect of sunlight on the nuclear polyhedrosis virus ofNeodiprion swainel with measurement of solar energy received.J. Invertebr. Pathol. 19 179–188 (1972)
Waldbauer, G. P., The consumption and utilization of food by insects.Adv. Insect Physiol. 3 229–282 (1968).
Finney, D. J.,Probit Analysis, Cambridge University Press, 318 pp (1952).
Biever, K. D. and Hostetter, D. L., Activity of the nuclear polyhedrosis virus of the cabbage looper evaluated at programmed temperature regimens.J. Invertebr. Pathol. 18 81–84 (1971).
Stairs, G. R., Quantitative differences in susceptibility to nuclear polyhedrosis virus among larval instars of forest tent caterpillar,Malacosoma disstria (Hubner).J. Invertebr. Pathol. 7 427–429 (1965).
Cantwell, G. E., Inactivation of biological insecticides by irradiation.J. Invertebr. Pathol. 9 138–140 (1967).
David, W. A. L., The effect of ultraviolet radiation of known wavelengths on a granulosis virus ofPieris brassicae.J. Invertebr. Pathol. 14 336–342 (1969).
Pawar, V. M., Structure, development and physico-chemical properties of the nuclear polyhedrosis virus ofspodoptera litura (Fabricius) and associated changes in the haemolympl of the host, Post Graduate School, Indian Agricultural Research Institute, New Delhi (Ph.D. Thesis) (1974).
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Part of the M.Sc. thesis submitted by U.K. to P.G. School, I.A.R.I.
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Komolpith, U., Ramakrishanan, N. Bioassay of nuclear polyhedrosis virus against larval stages ofSpodoptera litura (Fabricius) and the effect of protectants against ultraviolet light. Proc. Indian Acad. Sci. 82, 195–203 (1975). https://doi.org/10.1007/BF03050532
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DOI: https://doi.org/10.1007/BF03050532