Summary
1. Hypomagnesemia was induced in weanling rats by feeding a magnesium-low diet. During a 78 day regimen on this diet, the animals showed retarded growth rate and low concentrations of magnesium in liver, kidney, skeletal muscle and heart but not in brain. When animals fed with the magnesium-low diet were returned to the control diet for 18 days, the Mg content of serum, heart, liver and kidney increased appreciably. The Mg content of skeletal muscle did not increase on supplementation.
2. Marked inhibition of P:O ratios was noticed in mitochondria prepared from magnesium deficient rats. P:O ratios were resorted to normal by supplementation with Mg.
3. Hypomagnesemia produced as above also led to depletion of tissue proteins and mitochondrial proteins. On injecting valine-1-14C such animals incorporatedin vivo significantly less of radioactivity into tissue and mitochondrial proteins. This defect was also partially corrected on returning the animals to the control diet.
4. Mitochondrial fractions prepored from tissues of weanling rats receiving the magnesium-low diet for 60 days showed increased swelling as evidenced by their differential response to calcium pentachlorophenol, phloridzin, orp-chloromercuri benzoate as compared to mitochondria prepared from tissues of control animals.
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Communicated by Dr. C. R. Krishna Murti, F.A.Sc.
Communication No. 1806 from the Central Drug Research Institute, Lucknow 226001.
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nee Amarlal, S.S. Effect of magnesium deficiency on oxidative phosphorylation, mitochondrial swelling and protein synthesis in weanling rats. Proc. Indian Acad. Sci. 82, 63–76 (1975). https://doi.org/10.1007/BF03050519
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DOI: https://doi.org/10.1007/BF03050519