Abstract
We tested the possibility of plantlet formation via somatic embryogenesis with leaf segments and mature zygotic embryos from a rare and endangered tree species,Oplopanax elatus. To induce calli, expiants were cultured under darkness in a solid MS medium containing 3% sucrose, 1g L-1 glutamine, and 0.3% gelrite. Treatment supplements included 2,4-D alone or in combination with thidiazuron. Generally, callus induction and growth were good from leaf expiants, whereas embryogenic calli could be induced only from zygotic embryos. These embryogenic calli were white or pale yellow and very friable. ABA and activated charcoal appeared to be important factors when inducing somatic embryos, with optimum levels being 0.1 mg L-1 and 0.02%, respectively. Many somatic embryos showed abnormalities during their development on the germination medium, but 35% could be converted if placed on a medium containing gibberellic acid (GA3). The germinating embryos sometimes formed secondary embryos at the lower portion of the hypocotyls. Normal or converted plantlets were acclimatized in an artificial soil mixture; their survival was about 60% after two months. This culturing system provides a feasible approach for regenerating plants, via somatic embryogenesis, from mature zygotic embryos.
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Literature cited
Augustine AC, D’Souza L (1997) Somatic embryogenesis in Gnetumula Brongn. (Cnetum edule) (Willd) Blume. Plant Cell Rep16: 354–357
Beena MR, Martin KP (2003)In vitro propagation of the rare medicinal plantCeropegia candelabrum L. through somatic embryogenesis.In Vitro Cell Dev Biol Plant39: 510–513
Bodhipadma K, Leung DWM (2002) Factors important for somatic embryogenesis in zygotic embryo expiants ofCapsicum annuum L J Plant Biol45: 49–55
Bonga JM (2004) The effect of various culture media on the formation of embryo-like structures in cultures derived from expiants taken from matureLarix deciduas. Plant Cell Tiss Org Cult77: 43–48
Bonneau L, Beranger-Novat N, Monin J (1994) Somatic embryogenesis and plant regeneration in a woody species: The European spindle tree (Euonymus europaeus L). Plant Cell Rep13: 135–138
Capuana M, Debergh PC (1997) Improvement of the maturation and germination of horse chestnut somatic embryos. Plant Cell Tiss Org Cult48: 23–29
Chalupa V (2000)In vitro propagation of mature trees of pedunculate oak (Quercus robur L). J Forest Sci46: 537–542
Cho HJ, Yang WY, Yun DW, Kim YH, Son WY, Chung TY (1991) Somatic embryogenesis from inflorescence derived callus ofOplopanax elatus Nakai. Res Rep RDA B Kor33: 1–6
Choi YE, Jeong JH (2002) Dormancy induction of somatic embryos of Siberian ginseng by high sucrose concentrations enhances the conservation of hydrated artificial seeds and dehydrated resistance. Plant Cell Rep20: 1112–1116
Dai J, Vendrame WA, Merkle SA (2004) Enhancing the productivity of hybrid yellow-poplar and hybrid sweetgum embryogenie cultures.In Vitro Cell Dev Biol Plant40: 376–383
Das P, Samantaray S, Roberts AV, Rout GR (1997)In vitro somatic embryogenesis ofDalbergia sissoo Roxb.- a multipurpose timber-yielding tree. Plant Cell Rep16: 578–582
Feher A, Taras P, Pasternak P, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tiss Org Cult74: 201–228
Fu LK, Jin J (1992) China Plant Red Data Book: Rare and Endangered Plants. Science Press, Beijing, pp 741
Comes GAC, Paiva R, Paiva PDDO, Santiago EJAD (2003) Plant regeneration from callus cultures ofMadura tinctoria, an endangered woody species.In Vitro Cell Dev Biol Plant39: 293–295
Hackman I, von Arnolds (1988) Somatic embryogenesis and plant regeneration from suspension cultures ofPicea glauca (white spruce). Physiol Plant72: 579–587
Hussein S, Ibrahim R, Kiong ALP (2006) Potential biochemical markers for somatic embryos ofEurycoma logifolia Jack. J Plant Biol49: 97–101
Jimenez VM (2005) Involvement of plant hormones and plant growth regulators onin vitro somatic embryogenesis. Plant Growth Regul47: 91–110
Kim SW, Oh SC, Liu JR (2003) Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose. Plant Cell Tiss Org Cult74: 61–66
Kowalski B, von Staden J (2001)In vitro culture of two threatened South African medicinal trees —Ocotea bullata andWarburgia salutaris. Plant Growth Regul34: 223–228
Lee SW, Kim YM, Kim WW, Chung JM (2002) Genetic variation of I-SSR markers in the natural populations of a rare and endangered tree species, Op/opanaxelatus in Korea. J Kor For Soc91: 565–573
Martin KP (2003a) Rapidin vitro multiplication and exvitro rooting ofRotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep21: 415–420
Martin KP (2003b) Plant regeneration through somatic embryogenesis onHolostemma adakodien, a rare medicinal plant. Plant Cell Tiss Org Cult72: 79–82
Merkle SA, Bailey RL, Pauley BA, Neu KA, Kim MK, Rugu CL, Montello PM (1997) Somatic embryogenesis from tissues of mature sweetgum trees. Can J For Res27: 959–964
Merkle SA, Battle PJ (2000) Enhancement of embryogenic culture initiation from tissues of mature sweetgum trees. Plant Cell Rep19: 268–273
Merkle SA, Battle PJ, Ware GO (2003) Factors influencing production of inflorescence-derived somatic seedlings of sweetgum. Plant Cell Tiss Org Cult73: 95–99
Merkle SA, Neu KA, Battle PJ, Bailey RL (1998) Somatic embryogenesis and plantlet regeneration from immature and mature tissues of sweetgum (Liquidambar styraciflua). Plant Sci132: 169–178
Moon HK, Kim YW, Lee JS, Choi YE (2005) Micropropagation ofKalopanax pictus tree via somatic embryogenesis.In Vitro Cell Dev Biol Plant41: 303–306
Moon HK, Youn Y (1999) Somatic embryogenesis from winter buds of 10-year-oldAralia elata, In SM Jain, PK Gupta, RJ Newton, eds, Somatic Embryogenesis in Woody Plants. Vol 5, Kluwer, Dordrecht, pp 129–134
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol Plant15: 473–479
Nanda RM, Rout GR (2003)In vitro somatic embryogenesis and plant regeneration inAcacia arabica. Plant Cell Tiss Org Cult73: 131–135
Negash L (2002) Successful vegetative propagation techniques for the threatened African pencil cedar (juniperus procera Hoechst. Ex Endl.). For Ecol Manag161: 53–64
Raemakers K, Jacobsen E, Visser R (1999) Proliferative somatic embryogenesis in woody species,In SM Jain, PK Gupta, RJ Newton, eds, Somatic Embryogenesis in Woody Plants. Vol 4, Kluwer, Dordrecht, pp 29–59
Rai VR (2002) Rapid clonal propagation ofNothapodytes foetida (Wight) Sleumer-a threatened medicinal tree.In Vitro Cell Dev Biol Plant38: 347–351
Sharp WR, Sondahl MR, Caldas LS, Maraffa SB (1980) The physiology ofin vitro asexual embryogenesis. Hortic Rev2: 268–310
Sutton BSC, Polonenko DR (1999) Commercialization of plant somatic embryogenesis,In SM Jain, PK Gupta, RJ Newton, eds, Somatic Embryogenesis in Woody Plants. Vol 4, Kluwer, Dordrecht, pp 263–291
Tonon G, Berardi G, Rossi C, Bagnaresi U (2001) Synchronized somatic embryo development in embryogenic suspensions ofFraxinus angustifolia. In Vitro Cell Dev Biol Plant37: 462–465
Vendrame WA, Holliday CR Merkle SA (2001) Clonal propagation of hybrid sweetgum (Liquidambar styraciflua ×L. formosana) by somatic embryogenesis. Plant Cell Rep20: 691–695
Vookova B, Matusova R, Kormutak A (2003) Secondary somatic embryogenesis inAbies numidica. Biol Plant46: 513–517
ZhuravlevYu N, Kolyada AS (1996) Araliaceae: Ginseng and Other Aralia of the Russian Far East. Dalnauka Press, Vladivostok
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Moon, H.K., Kim, J.A., Park, S.Y. et al. Somatic embryogenesis and plantlet formation from a rare and endangered tree species,Oplopanax elatus . J. Plant Biol. 49, 320–325 (2006). https://doi.org/10.1007/BF03031163
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DOI: https://doi.org/10.1007/BF03031163