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Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation

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Abstract

We evaluated the efficiency of callus induction and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were ‘Marathon’, ‘Greenbelt’, and ‘Shogun’. Transformation success was not affected by the presence of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls than from 14-d-old hypocotyls. Both ‘Marathon’ and ‘Greenbelt’ had higher potentials for tissue regeneration than did ‘Shogun’. We found that for transformation selection, the optimum concentration was either 50 mg/L kanamycin or 100 mg/L genetkin.

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Authors and Affiliations

  1. Department of Biological Sciences, Kyungnam University, 630-701, Masan, Korea

    Jong Hee Kim

  2. Department of Botany, University of Queensland, Brisbane, Australia

    J. R. Botella

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  1. Jong Hee Kim
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  2. J. R. Botella
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Correspondence to Jong Hee Kim.

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Kim, J.H., Botella, J.R. Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation. J. Plant Biol. 45, 177–181 (2002). https://doi.org/10.1007/BF03030311

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  • DOI: https://doi.org/10.1007/BF03030311

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